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Rabbit Anti-EIF3J Recombinant Antibody (CBFYE-0686) (CBMAB-E1107-FY)

This product is rabbit antibody that recognizes EIF3J. The antibody CBFYE-0686 can be used for immunoassay techniques such as: WB, IP, IF.
See all EIF3J antibodies

Summary

Host Animal
Rabbit
Specificity
Human, Mouse, Rat, Monkey
Clone
CBFYE-0686
Antibody Isotype
IgG
Application
WB, IP, IF

Basic Information

Specificity
Human, Mouse, Rat, Monkey
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Eukaryotic Translation Initiation Factor 3 Subunit J
Introduction
This gene encodes a core subunit of the eukaryotic initiation factor 3 complex, which participates in the initiation of translation by aiding in the recruitment of protein and mRNA components to the 40S ribosome. There are pseudogenes for this gene on chromosomes 1, 3, and 9. Alternative splicing results in multiple transcript variants encoding different isoforms.
Entrez Gene ID
Human8669
Mouse78655
Rat691947
Monkey712295
UniProt ID
HumanO75822
MouseQ3UGC7
RatA0JPM9
MonkeyI2CW27
Alternative Names
Eukaryotic Translation Initiation Factor 3 Subunit J; EIF3S1; Eukaryotic Translation Initiation Factor 3, Subunit 1 (Alpha, 35kD); Eukaryotic Translation Initiation Factor 3, Subunit 1 Alpha, 35kDa; Eukaryotic Translation Initiation Factor 3, Subunit J; Eukaryotic Translation Initiation Factor 3 Subunit 1
Research Area
Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:25849773, PubMed:27462815).

The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).
Biological Process
Formation of cytoplasmic translation initiation complex Source: UniProtKB-UniRule
Translational initiation Source: UniProtKB
Cellular Location
Cytoplasm
PTM
Phosphorylated. Phosphorylation is enhanced upon serum stimulation.

Ye, C., Qin, S., Guo, F., Yang, Y., Wang, H., Zhang, C., & Yang, B. (2022). LncRNA EIF3J-AS1 functions as an oncogene by regulating MAFG to promote prostate cancer progression. Journal of Cancer, 13(1), 146.

Egorova, T., Biziaev, N., Shuvalov, A., Sokolova, E., Mukba, S., Evmenov, K., ... & Alkalaeva, E. (2021). eIF3j facilitates loading of release factors into the ribosome. Nucleic acids research, 49(19), 11181-11196.

Luo, Y., Zheng, S., Wu, Q., Wu, J., Zhou, R., Wang, C., ... & Liao, W. (2021). Long noncoding RNA (lncRNA) EIF3J-DT induces chemoresistance of gastric cancer via autophagy activation. Autophagy, 17(12), 4083-4101.

Liu, D., Zhang, H., Cong, J., Cui, M., Ma, M., Zhang, F., ... & Chen, C. (2020). H3K27 acetylation‐induced lncRNA EIF3J‐AS1 improved proliferation and impeded apoptosis of colorectal cancer through miR‐3163/YAP1 axis. Journal of cellular biochemistry, 121(2), 1923-1933.

Wei, W. T., Wang, L., Liang, J. X., Wang, J. F., Li, Q., & Zeng, J. (2020). LncRNA EIF3J-AS1 enhanced esophageal cancer invasion via regulating AKT1 expression through sponging miR-373-3p. Scientific Reports, 10(1), 1-9.

Qi, J., Wang, Z., Zhao, Z., & Liu, L. (2020). EIF3J-AS1 promotes glioma cell growth via up-regulating ANXA11 through sponging miR-1343-3p. Cancer Cell International, 20(1), 1-13.

Yang, X., Yao, B., Niu, Y., Chen, T., Mo, H., Wang, L., ... & Yao, D. (2019). Hypoxia-induced lncRNA EIF3J-AS1 accelerates hepatocellular carcinoma progression via targeting miR-122–5p/CTNND2 axis. Biochemical and Biophysical Research Communications, 518(2), 239-245.

Young, D. J., & Guydosh, N. R. (2019). Hcr1/eIF3j is a 60S ribosomal subunit recycling accessory factor in vivo. Cell reports, 28(1), 39-50.

Chen, X., Yang, Z., Yang, C., Xie, K., Sun, W., & Xie, S. (2018). Sparse gene Coexpression network analysis reveals EIF3J-AS1 as a prognostic marker for breast Cancer. Complexity, 2018.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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