Mouse Anti-EIF4G2 Recombinant Antibody (CBFYE-0740) (V2LY-0425-LY1775)

Name: Mouse Anti-EIF4G2 Recombinant Antibody (CBFYE-0740)
SKU: V2LY-0425-LY1775
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-EIF4G2 Recombinant Antibody (CBFYE-0740) (V2LY-0425-LY1775-1) in WB

Western-blotting of Wnt2 in the contralateral and ipsilateral cortex infected by lentivirus expressing DAP5 shRNA or control shRNA. *P=0.039. One-way ANOVA followed by Bonferroni's post hoc comparisons tests. N=3 mice per group. ...View More

Fan, H., Yang, J., Zhang, K., Xing, J., Guo, B., Mao, H., ... & Wang, Y. (2022). IRES-mediated Wnt2 translation in apoptotic neurons triggers astrocyte dedifferentiation. NPJ Regenerative Medicine, 7(1), 42.

Mouse Anti-EIF4G2 Recombinant Antibody (CBFYE-0740) (V2LY-0425-LY1775-2) in IF

Double-immunostaining and quantification of GFAP/β-gal in ischemic cortex treated by control shRNA and DAP5 shRNA. Notice the attenuated expression of β-gal in DAP5 shRNA treated cortex. **P=0.0081. Two-tailed Student's t test. N=3 mice per group. Double-immunostaining and quantification of GFAP/Nestin in ischemic cortex treated by control shRNA and DAP5 shRNA. Ischemia-induced Nestin was reduced in DAP5 shRNA treated cortex. **P=0.0097. Two-tailed Student's t test. N=3 mice per group. Double-immunostaining of DCX/BrdU in ischemic cortex treated by control shRNA and DAP5 shRNA. Notice the decreased number of DCX/BrdU-positive cells in DAP5 shRNA treated cortex. ...View More

Mouse Anti-EIF4G2 Recombinant Antibody (CBFYE-0740) (V2LY-0425-LY1775-3) in WB

Western blotting of eIF4GI- and DAP5-depleted RRL probed using specific eIF4GI and DAP5 antibodies. Purified recombinant eIF4GI682-1599 or DAP5 protein used for the study were included as positive controls (lane 1). ...View More

Haizel, S. A., Bhardwaj, U., Gonzalez, R. L., Mitra, S., & Goss, D. J. (2020). 5'-UTR recruitment of the translation initiation factor eIF4GI or DAP5 drives cap-independent translation of a subset of human mRNAs. Journal of Biological Chemistry, 295(33), 11693-11706.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0740

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 608-907 mapping at the C-terminus of human DAP-5.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium Azide

Concentration

0.2 mg/ml

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Eukaryotic Translation Initiation Factor 4 Gamma 2

Entrez Gene ID

Human	1982
Mouse	13690
Rat	361628

UniProt ID

Human	P78344
Mouse	Q62448
Rat	F1LN59

Research Area

Appears to play a role in the switch from cap-dependent to IRES-mediated translation during mitosis, apoptosis and viral infection. Cleaved by some caspases and viral proteases.

Biological Process

Cell death Source: ProtInc
Cellular macromolecule biosynthetic process Source: ParkinsonsUK-UCL
Heart development Source: Ensembl
Negative regulation of autophagy Source: ParkinsonsUK-UCL
Positive regulation of axon extension Source: Ensembl
Positive regulation of cell growth Source: ParkinsonsUK-UCL
Positive regulation of dendritic spine development Source: Ensembl
Positive regulation of translation Source: Ensembl
Regulation of cell cycle Source: ProtInc
Regulation of translational initiation Source: UniProtKB

Cellular Location

Cytosol; Adherens junction; Axon; Eukaryotic translation initiation factor 4F complex; Membrane

PTM

Phosphorylation; hyperphosphorylated during mitosis.

Smirnova, V. V., Shestakova, E. D., Nogina, D. S., Mishchenko, P. A., Prikazchikova, T. A., Zatsepin, T. S., ... & Terenin, I. M. (2022). Ribosomal leaky scanning through a translated uORF requires eIF4G2. Nucleic acids research, 50(2), 1111-1127.

Chen, K., Xi, M., Huang, Q., Wu, H., Lu, G., Song, S., & Shi, W. (2022). Long non-coding RNA MCM3AP antisense RNA 1 silencing upregulates microRNA-24-3p to accelerate proliferation and migration of vascular endothelial cells in myocardial infarction rats by reducing EIF4G2. Cell Cycle, 21(7), 674-684.

Li, S., Shao, J., Lou, G., Wu, C., Liu, Y., & Zheng, M. (2021). MiR-144-3p-mediated dysregulation of EIF4G2 contributes to the development of hepatocellular carcinoma through the ERK pathway. Journal of Experimental & Clinical Cancer Research, 40(1), 1-14.

Zhang, J., Zhang, R., & Ye, Y. (2021). Long non-coding RNA (LncRNA) SNHG7/Eukaryotic translation initiation factor 4 gamma 2 (EIF4G2) involves in the malignant events of ovarian cancer cells with paclitaxel resistant. Bioengineered, 12(2), 10541-10552.

Zhao, H., Wang, A., & Zhang, Z. (2020). LncRNA SDHAP1 confers paclitaxel resistance of ovarian cancer by regulating EIF4G2 expression via miR-4465. The Journal of Biochemistry, 168(2), 171-181.

Gao, S., Liu, L., Zhu, S., Wang, D., Wu, Q., Ning, G., & Feng, S. (2020). MicroRNA-197 regulates chondrocyte proliferation, migration, and inflammation in pathogenesis of osteoarthritis by targeting EIF4G2. Bioscience Reports, 40(9).

Wang, J., Lin, Z., Yang, Z., & Liu, X. (2020). lncRNA Eif4g2 improves palmitate-induced dysfunction of mouse β-cells via modulation of Nrf2 activation. Experimental cell research, 396(2), 112291.

Zhang, X., Sun, Z., & Zou, Y. (2020). LncRNA NEAT1 exacerbates non-small cell lung cancer by upregulating EIF4G2 via miR-582-5p sponging. Archives of Biological Sciences, 72(2), 243-252.

Chai, Y., & Xie, M. (2019). LINC01579 promotes cell proliferation by acting as a ceRNA of miR‐139‐5p to upregulate EIF4G2 expression in glioblastoma. Journal of cellular physiology, 234(12), 23658-23666.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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