Mouse Anti-ELF1 Recombinant Antibody (3B7) (CBMAB-A2565-LY)

Name: Mouse Anti-ELF1 Recombinant Antibody (3B7)
SKU: CBMAB-A2565-LY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

3B7

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 595-619 mapping at the C-terminus of human Elf-1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

E74 Like ETS Transcription Factor 1

Introduction

This gene encodes an E26 transformation-specific related transcription factor. The encoded protein is primarily expressed in lymphoid cells and acts as both an enhancer and a repressor to regulate transcription of various genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

Entrez Gene ID

Human	1997
Mouse	13709
Rat	85424

UniProt ID

Human	P32519
Mouse	Q60775
Rat	Q9EQY3

Research Area

Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer.

Biological Process

Cell differentiation Source: GO_Central
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central

Cellular Location

Nucleus

Qiao, C., Qiao, T., Yang, S., Liu, L., & Zheng, M. (2022). SNHG17/miR-384/ELF1 axis promotes cell growth by transcriptional regulation of CTNNB1 to activate Wnt/β-catenin pathway in oral squamous cell carcinoma. Cancer Gene Therapy, 29(1), 122-132.

Liu, H., Liu, L., Liu, Q., He, F., & Zhu, H. (2022). LncRNA HOXD-AS1 affects proliferation and apoptosis of cervical cancer cells by promoting FRRS1 expression via transcription factor ELF1. Cell Cycle, 21(4), 416-426.

Hu, M., Li, H., Xie, H., Fan, M., Wang, J., Zhang, N., ... & Che, S. (2021). ELF1 transcription factor enhances the Progression of glioma via ATF5 promoter. ACS Chemical Neuroscience, 12(7), 1252-1261.

Xu, J., Chong, J., & Wang, D. (2021). Opposite roles of transcription elongation factors Spt4/5 and Elf1 in RNA polymerase II transcription through B-form versus non-B DNA structures. Nucleic acids research, 49(9), 4944-4953.

Cheng, M., Zeng, Y., Zhang, T., Xu, M., Li, Z., & Wu, Y. (2021). Transcription factor ELF1 activates MEIS1 transcription and then regulates the GFI1/FBW7 axis to promote the development of glioma. Molecular Therapy-Nucleic Acids, 23, 418-430.

Wang, L. (2021). ELF1-activated FOXD3-AS1 promotes the migration, invasion and EMT of osteosarcoma cells via sponging miR-296-5p to upregulate ZCCHC3. Journal of bone oncology, 26, 100335.

Wang, M., Yang, C., Liu, X., Zheng, J., Xue, Y., Ruan, X., ... & Liu, Y. (2020). An upstream open reading frame regulates vasculogenic mimicry of glioma via ZNRD1‐AS1/miR‐499a‐5p/ELF1/EMI1 pathway. Journal of cellular and molecular medicine, 24(11), 6120-6136.

Wang, L., Tang, D., Wu, T., & Sun, F. (2020). ELF1‐mediated LUCAT1 promotes choroidal melanoma by modulating RBX1 expression. Cancer Medicine, 9(6), 2160-2170.

Ehara, H., & Sekine, S. I. (2018). Architecture of the RNA polymerase II elongation complex: new insights into Spt4/5 and Elf1. Transcription, 9(5), 286-291.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Antibody Pairs

ELF1 Matched Antibody Pair (373) (CAT#: APMAB-373LY)

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ELF1 Matched Antibody Pair (373) (CAT#: APMAB-373LY)

Mouse Anti-ELF1 Recombinant Antibody (CBFYE-0765) (CAT#: CBMAB-E1209-FY)

Mouse Anti-ELF1 Recombinant Antibody (CBFYE-0199) (CAT#: CBMAB-E0444-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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