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Mouse Anti-ELL Recombinant Antibody (23) (CBMAB-E1236-FY)

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Datasheet
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Basic Information

Host Animal
Mouse
Clone
23
Application
WB, IP
Immunogen
Recombinant protein corresponding to an internal region of human ELL.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG1
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, gelatin
Preservative
Sodium azide
Concentration
0.1 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Elongation Factor For RNA Polymerase II
Introduction
ELL (Elongation Factor For RNA Polymerase II) is a Protein Coding gene. Diseases associated with ELL include Leukemia, Acute Myeloid. Among its related pathways are Formation of HIV elongation complex in the absence of HIV Tat and Gene Expression. Gene Ontology (GO) annotations related to this gene include phosphatase binding. An important paralog of this gene is ELL2.
Entrez Gene ID
8178
UniProt ID
P55199
Alternative Names
Elongation Factor For RNA Polymerase II; Protein Phosphatase 1, Regulatory Subunit 68; Eleven-Nineteen Lysine-Rich Leukemia Protein; Elongation Factor RNA Polymerase II; C19orf17; ELL Gene (11-19 Lysine-Rich Leukemia Gene); Eleven-Nineteen Lysine-Rich Leukemia Gene
Research Area
Elongation factor component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. Elongation factor component of the little elongation complex (LEC), a complex required to regulate small nuclear RNA (snRNA) gene transcription by RNA polymerase II and III (PubMed:22195968, PubMed:23932780).

Specifically required for stimulating the elongation step of RNA polymerase II- and III-dependent snRNA gene transcription (PubMed:23932780).

ELL also plays an early role before its assembly into in the SEC complex by stabilizing RNA polymerase II recruitment/initiation and entry into the pause site. Required to stabilize the pre-initiation complex and early elongation.
Biological Process
In utero embryonic development Source: Ensembl
Positive regulation of DNA-templated transcription, elongation Source: ProtInc
Positive regulation of transcription by RNA polymerase III Source: UniProtKB
Positive regulation of transcription elongation from RNA polymerase II promoter Source: UniProtKB
snRNA transcription by RNA polymerase II Source: UniProtKB
snRNA transcription by RNA polymerase III Source: UniProtKB
Transcription elongation from RNA polymerase II promoter Source: ProtInc
Cellular Location
Nucleus; Nucleus speckle; Cajal body. Colocalizes with EAF2 to nuclear speckles (PubMed:12446457). Colocalizes with coilin in subnuclear cajal and histone locus bodies (PubMed:12686606). Translocates in the LEC complex to cajal and histone locus bodies at snRNA genes in a ICE1-dependent manner. Associates to transcriptionally active chromatin at snRNA genes(PubMed:23932780).
Involvement in disease
A chromosomal aberration involving ELL is found in acute leukemias. Translocation t(11;19)(q23;p13.1) with KMT2A/MLL1. The result is a rogue activator protein.

Sweta, K., Dabas, P., & Sharma, N. (2022). Sequence, structural and functional conservation among the human and fission yeast ELL and EAF transcription elongation factors. Molecular Biology Reports, 49(2), 1303-1320.

Fei, J., Xu, J., Li, Z., Xu, K., Wang, D., Kassavetis, G. A., & Kadonaga, J. T. (2022). NDF is a transcription factor that stimulates elongation by RNA polymerase II. Genes & Development, 36(5-6), 294-299.

Li, J., Bansal, V., Tiwari, M., Chen, Y., & Sen, G. L. (2021). ELL facilitates RNA polymerase II-mediated transcription of human epidermal proliferation genes. The Journal of investigative dermatology, 141(5), 1352-1356.

Sweta, K., & Sharma, N. (2021). Functional interaction between ELL transcription elongation factor and Epe1 reveals the role of Epe1 in the regulation of transcription outside heterochromatin. Molecular Microbiology, 116(1), 80-96.

Basu, S., Nandy, A., & Biswas, D. (2020). Keeping RNA polymerase II on the run: Functions of MLL fusion partners in transcriptional regulation. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 1863(8), 194563.

Chen, F. X., Smith, E. R., & Shilatifard, A. (2018). Born to run: control of transcription elongation by RNA polymerase II. Nature reviews Molecular cell biology, 19(7), 464-478.

Gopalan, S., Gibbon, D. M., Banks, C. A., Zhang, Y., Florens, L. A., Washburn, M. P., ... & Conaway, J. W. (2018). Schizosaccharomyces pombe Pol II transcription elongation factor ELL functions as part of a rudimentary super elongation complex. Nucleic acids research, 46(19), 10095-10105.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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