Mouse Anti-ELN Recombinant Antibody (B4) (CBMAB-A4383-YC)

Name: Mouse Anti-ELN Recombinant Antibody (B4)
SKU: CBMAB-A4383-YC
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 431-730 of human elastin.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Elastin

Introduction

ELN is a protein that is one of the two components of elastic fibers. Elastic fibers comprise part of the extracellular matrix and confer elasticity to organs and tissues including the heart, skin, lungs, ligaments, and blood vessels. The encoded protein

Entrez Gene ID

2006

UniProt ID

P15502

Alternative Names

AI385707; AI480567; E030024M20Rik

Research Area

Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle (By similarity).

Biological Process

Animal organ morphogenesis Source: ProtInc
Aortic valve morphogenesis Source: BHF-UCL
Blood circulation Source: ProtInc
Extracellular matrix organization Source: Ensembl
Outflow tract morphogenesis Source: BHF-UCL
Regulation of actin filament polymerization Source: Ensembl
Regulation of smooth muscle cell proliferation Source: GO_Central
Respiratory gaseous exchange by respiratory system Source: ProtInc
Skeletal muscle tissue development Source: Ensembl
Stress fiber assembly Source: Ensembl

Cellular Location

Extracellular matrix. Extracellular matrix of elastic fibers.

Involvement in disease

Cutis laxa, autosomal dominant, 1 (ADCL1):
A connective tissue disorder characterized by loose, hyperextensible skin with decreased resilience and elasticity leading to a premature aged appearance. Face, hands, feet, joints, and torso may be differentially affected. Additional variable clinical features are gastrointestinal diverticula, hernia, and genital prolapse. Rare manifestations are pulmonary artery stenosis, aortic aneurysm, bronchiectasis, and emphysema.
Supravalvular aortic stenosis (SVAS):
Congenital narrowing of the ascending aorta which can occur sporadically, as an autosomal dominant condition, or as one component of Williams-Beuren syndrome.
ELN is located in the Williams-Beuren syndrome (WBS) critical region. WBS results from a hemizygous deletion of several genes on chromosome 7q11.23, thought to arise as a consequence of unequal crossing over between highly homologous low-copy repeat sequences flanking the deleted region. Haploinsufficiency of ELN may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in the disease (PubMed:8812460).

PTM

Elastin is formed through the cross-linking of its soluble precursor tropoelastin. Cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. Subsequent spontaneous condensation reactions with other allysine or unmodified lysine residues result in various bi-, tri-, and tetrafunctional cross-links. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine.
Hydroxylation on proline residues within the sequence motif, GXPG, is most likely 4-hydroxy as this fits the requirement for 4-hydroxylation in vertebrates.

Lin, C. J., Cocciolone, A. J., & Wagenseil, J. E. (2022). Elastin, arterial mechanics, and stenosis. American Journal of Physiology-Cell Physiology, 322(5), C875-C886.

Procknow, S. S., & Kozel, B. A. (2022). Emerging mechanisms of elastin transcriptional regulation. American Journal of Physiology-Cell Physiology, 323(3), C666-C677.

Kim, J., Cocciolone, A. J., Staiculescu, M. C., Mecham, R. P., & Wagenseil, J. E. (2022). Passive biaxial mechanical behavior of newborn mouse aorta with and without elastin. Journal of the Mechanical Behavior of Biomedical Materials, 126, 105021.

Peng, X., Guo, Z., Zhang, Y., Sun, B., & Zhang, Q. (2022). EFEMP1 in direct inguinal hernia: correlation with TIMP3 and regulation toward elastin homoeostasis as well as fibroblast mobility. Journal of Investigative Surgery, 35(1), 203-211.

Dave, J. M., Chakraborty, R., Ntokou, A., Saito, J., Saddouk, F. Z., Feng, Z., ... & Greif, D. M. (2022). JAGGED1/NOTCH3 activation promotes aortic hypermuscularization and stenosis in elastin deficiency. The Journal of clinical investigation, 132(5).

Lin, C. J., Hunkins, B. M., Roth, R. A., Lin, C. Y., Wagenseil, J. E., & Mecham, R. P. (2021). Vascular smooth muscle cell subpopulations and neointimal formation in mouse models of elastin insufficiency. Arteriosclerosis, thrombosis, and vascular biology, 41(12), 2890-2905.

Li, J., Xu, X., Jiang, Y., Hansbro, N. G., Hansbro, P. M., Xu, J., & Liu, G. (2020). Elastin is a key factor of tumor development in colorectal cancer. BMC cancer, 20(1), 1-12.

Kim, J., Cocciolone, A. J., Staiculescu, M. C., Mecham, R. P., & Wagenseil, J. E. (2020). Captopril treatment during development alleviates mechanically induced aortic remodeling in newborn elastin knockout mice. Biomechanics and modeling in mechanobiology, 19(1), 99-112.

Hayano, S., Okuno, Y., Tsutsumi, M., Inagaki, H., Fukasawa, Y., Kurahashi, H., ... & Kato, T. (2019). Frequent intragenic microdeletions of elastin in familial supravalvular aortic stenosis. International Journal of Cardiology, 274, 290-295.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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