Mouse Anti-ELOB Recombinant Antibody (CBYJT-1217) (CBMAB-T0232-YJ)

Name: Mouse Anti-ELOB Recombinant Antibody (CBYJT-1217)
SKU: CBMAB-T0232-YJ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBYJT-1217

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Full length of human Elongin B.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Elongin B

Introduction

ELOB is the protein elongin B, which is a subunit of the transcription factor B (SIII) complex. The SIII complex is composed of elongins A/A2, B and C. ELOB activates elongation by RNA polymerase II by suppressing transient pausing of the polymerase at many sites within transcription units. Elongin A plays a role as the transcriptionally active component of the SIII complex, whereas elongins B and C are regulatory subunits. Elongin A2 is specifically expressed in the testis, and capable of forming a stable complex with elongins B and C. The von Hippel-Lindau tumor suppressor protein binds to elongins B and C, and thereby inhibits transcription elongation. Two alternatively spliced transcript variants encoding different isoforms have been described for ELOB.

Entrez Gene ID

Human	6923
Rat	81807

UniProt ID

Human	Q15370
Rat	P62870

Alternative Names

Elongin B; Transcription Elongation Factor B (SIII), Polypeptide 2 (18kDa, Elongin B); RNA Polymerase II Transcription Factor SIII Subunit B; Transcription Elongation Factor B Polypeptide 2; Transcription Elongation Factor B Subunit 2; Elongin 18 KDa Subunit; SIII P18

Biological Process

Positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: FlyBase
Protein-containing complex assembly Source: ProtInc
Protein ubiquitination Source: UniProtKB-UniPathway
Transcription elongation from RNA polymerase II promoter Source: ProtInc

Cellular Location

Nucleus

Gossing, W., Radke, L., Biering, H., Diederich, S., Mai, K., & Frohme, M. (2021). The ElonginB/C-Cullin5-SOCS-Box-Complex Is a Potential Biomarker for Growth Hormone Disorders. Biomedicines 2021, 9, 201.

Zhao, Z., Li, Z., Huan, C., Wang, H., Su, X., & Zhang, W. (2019). CAEV Vif hijacks ElonginB/C, CYPA and Cullin5 to assemble the E3 ubiquitin ligase complex stepwise to degrade oaA3Z2-Z3. Frontiers in Microbiology, 10, 565.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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