Mouse Anti-ELOVL5 Recombinant Antibody (CBFYE-0804) (CBMAB-E1252-FY)

Name: Mouse Anti-ELOVL5 Recombinant Antibody (CBFYE-0804)
SKU: CBMAB-E1252-FY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-ELOVL5 Recombinant Antibody (CBFYE-0804) (CBMAB-E1252-FY) in WB

Western blots showing the expression levels of well-known ferroptosis regulators upon 0.2μM RSL3 and 2μM darapladib treatment as indicated. Three blots were cut according to protein size and directed to western blotting, and reprobed to detect proteins of similar size. Each protein was normalised to α-tubulin on the same blot. Experiments were repeated three times and the data are also presented as the means±SDs (n=3 independent experiments). ...View More

Oh, M., Jang, S. Y., Lee, J. Y., Kim, J. W., Jung, Y., Kim, J., ... & Lee, E. W. (2023). The lipoprotein-associated phospholipase A2 inhibitor Darapladib sensitises cancer cells to ferroptosis by remodelling lipid metabolism. Nature communications, 14(1), 5728.

Datasheet

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0804

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 244-277 near the C-terminus of rat ELOVL5.

Host Species

Mouse

Specificity

Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

ELOVL Fatty Acid Elongase 5

Introduction

This gene belongs to the ELO family. It is highly expressed in the adrenal gland and testis, and encodes a multi-pass membrane protein that is localized in the endoplasmic reticulum. This protein is involved in the elongation of long-chain polyunsaturated fatty acids. Mutations in this gene have been associated with spinocerebellar ataxia-38 (SCA38). Alternatively spliced transcript variants have been found for this gene.

Entrez Gene ID

Rat	171400
Mouse	68801

UniProt ID

Rat	D4A9V6
Mouse	Q920L7

Alternative Names

HELO1; AI747313; AU043003; 1110059L23Rik

Research Area

Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that acts specifically toward polyunsaturated acyl-CoA with the higher activity toward C18:3(n-6) acyl-CoA. May participate in the production of monounsaturated and of polyunsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators (By similarity) (PubMed:10970790, PubMed:20937905).

In conditions where the essential linoleic and alpha linoleic fatty acids are lacking it is also involved in the synthesis of Mead acid from oleic acid (By similarity).

Biological Process

Alpha-linolenic acid metabolic process Source: Reactome
Fatty acid elongation, monounsaturated fatty acid Source: UniProtKB
Fatty acid elongation, polyunsaturated fatty acid Source: UniProtKB
Fatty acid elongation, saturated fatty acid Source: GO_Central
Linoleic acid metabolic process Source: Reactome
Long-chain fatty-acyl-CoA biosynthetic process Source: Reactome
Positive regulation of fatty acid biosynthetic process Source: BHF-UCL
Sphingolipid biosynthetic process Source: GO_Central
Unsaturated fatty acid biosynthetic process Source: UniProtKB-UniRule
Very long-chain fatty acid biosynthetic process Source: UniProtKB

Cellular Location

Endoplasmic reticulum membrane; Dendrite. In Purkinje cells, the protein localizes to the soma and proximal portion of the dendritic tree.

Involvement in disease

Spinocerebellar ataxia 38 (SCA38):
A form of spinocerebellar ataxia, a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCA38 is an autosomal dominant form characterized by adult-onset of slowly progressive gait ataxia accompanied by nystagmus. Brain MRI shows cerebellar atrophy.

Kieu, T. L. V., Pierre, L., Derangère, V., Perrey, S., Truntzer, C., Jalil, A., ... & Rialland, M. (2022). Downregulation of Elovl5 promotes breast cancer metastasis through a lipid-droplet accumulation-mediated induction of TGF-β receptors. Cell death & disease, 13(9), 1-18.

Li, Y., Tocher, D. R., Pang, Y., Du, J., Xiang, X., Mai, K., & Ai, Q. (2022). Environmental adaptation in fish induced changes in the regulatory region of fatty acid elongase gene, elovl5, involved in long-chain polyunsaturated fatty acid biosynthesis. International Journal of Biological Macromolecules, 204, 144-153.

Nitta, S., Kandori, S., Tanaka, K., Sakka, S., Siga, M., Nagumo, Y., ... & Nishiyama, H. (2022). ELOVL5‐mediated fatty acid elongation promotes cellular proliferation and invasion in renal cell carcinoma. Cancer science, 113(8), 2738.

Balbo, I., Montarolo, F., Genovese, F., Tempia, F., & Hoxha, E. (2022). Effects of the administration of Elovl5-dependent fatty acids on a spino-cerebellar ataxia 38 mouse model. Behavioral and Brain Functions, 18(1), 1-10.

Zhao, L., Li, F., Liu, T., Yuan, L., Zhang, X., Zhang, D., ... & Wang, W. (2022). Ovine ELOVL5 and FASN genes polymorphisms and their correlations with sheep tail fat deposition. Gene, 807, 145954.

Centenera, M. M., Scott, J. S., Machiels, J., Nassar, Z. D., Miller, D. C., Zinonos, I., ... & Butler, L. M. (2021). ELOVL5 Is a Critical and Targetable Fatty Acid Elongase in Prostate CancerTargeting ELOVL5 in Prostate Cancer. Cancer research, 81(7), 1704-1718.

Hoxha, E., Balbo, I., Parolisi, R., Audano, M., Montarolo, F., Ravera, F., ... & Tempia, F. (2021). Elovl5 is required for proper action potential conduction along peripheral myelinated fibers. Glia, 69(10), 2419-2428.

Sun, S., Ren, T., Li, X., Cao, X., & Gao, J. (2020). Polyunsaturated fatty acids synthesized by freshwater fish: A new insight to the roles of elovl2 and elovl5 in vivo. Biochemical and Biophysical Research Communications, 532(3), 414-419.

Losol, P., Rezwan, F. I., Patil, V. K., Venter, C., Ewart, S., Zhang, H., ... & Holloway, J. W. (2019). Effect of gestational oily fish intake on the risk of allergy in children may be influenced by FADS1/2, ELOVL5 expression and DNA methylation. Genes & Nutrition, 14(1), 1-7.

Shi, H. B., Du, Y., Zhang, C. H., Sun, C., He, Y. L., Wu, Y. H., ... & Loor, J. J. (2018). Fatty acid elongase 5 (ELOVL5) alters the synthesis of long-chain unsaturated fatty acids in goat mammary epithelial cells. Journal of dairy science, 101(5), 4586-4594.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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