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Rabbit Anti-EMC4 Recombinant Antibody (CBYJT-1221) (CBMAB-T0236-YJ)

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Rabbit
Clone
CBYJT-1221
Application
WB, IHC-P
Immunogen
Synthetic peptide within human grsf1 aa 21-70.
Host Species
Rabbit
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
WB1:1,000-1:5,000
IHC-P1:200-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
TBS, glycerol, BSA
Preservative
Sodium azide
Concentration
1 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
Epitope
aa 1-100
More Infomation

Target

Full Name
ER Membrane Protein Complex Subunit 4
Introduction
EMC4 (ER Membrane Protein Complex Subunit 4) functions in apoptotic process, protein folding in endoplasmic reticulum
Entrez Gene ID
Human51234
Mouse68032
Rat296049
UniProt ID
HumanQ5J8M3
MouseQ9CZX9
RatD4A0W1
Alternative Names
ER Membrane Protein Complex Subunit 4; Transmembrane Protein 85; Cell Proliferation-Inducing Gene 17 Protein; TMEM85; PIG17
Research Area
Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins (PubMed:30415835, PubMed:29809151, PubMed:29242231, PubMed:32459176, PubMed:32439656).

Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues (PubMed:30415835, PubMed:29809151, PubMed:29242231).

Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices (PubMed:30415835, PubMed:29809151).

It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes (PubMed:29809151, PubMed:29242231).

By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors (PubMed:30415835).

By regulating the insertion of various proteins in membranes, it is indirectly involved in many cellular processes (Probable).
Biological Process
Apoptotic process Source: UniProtKB-KW
Protein insertion into ER membrane by stop-transfer membrane-anchor sequence Source: UniProtKB
Tail-anchored membrane protein insertion into ER membrane Source: UniProtKB
Cellular Location
Endoplasmic reticulum membrane. Could also be a single-pass transmembrane protein with cytosolic N-terminus and lumenal C-terminus.
Topology
Cytoplasmic: 2-66
Helical: 67-87
Lumenal: 88-98
Helical: 99-120
Cytoplasmic: 121-127
Helical: 128-148
Lumenal: 149-183

Iyer, A., Niemann, M., Serricchio, M., Dewar, C. E., Oeljeklaus, S., Farine, L., ... & Bütikofer, P. (2022). The endoplasmic reticulum membrane protein complex localizes to the mitochondrial-endoplasmic reticulum interface and its subunits modulate phospholipid biosynthesis in Trypanosoma brucei. PLoS pathogens, 18(5), e1009717.

Sharma, S., Sourirajan, A., Baumler, D. J., & Dev, K. (2020). Saccharomyces cerevisiae ER membrane protein complex subunit 4 (EMC4) plays a crucial role in eIF2B-mediated translation regulation and survival under stress conditions. Journal of Genetic Engineering and Biotechnology, 18(1), 1-10.

Bai, L., You, Q., Feng, X., Kovach, A., & Li, H. (2020). Structure of the ER membrane complex, a transmembrane-domain insertase. Nature, 584(7821), 475-478.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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