Mouse Anti-EML1 Recombinant Antibody (CBFYE-0827) (V2LY-0825-LY170)

Name: Mouse Anti-EML1 Recombinant Antibody (CBFYE-0827)
SKU: V2LY-0825-LY170
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-EML1 Recombinant Antibody (CBFYE-0827) (V2LY-0825-LY170) in IF

Immunocytochemical detection of EML1 in skin fibroblasts derived from the RSH-affected patient (P) and controls (C1; C2 similar results, data not shown) was performed with antibodies against EML1 (green, PA5-21294,), α-tubulin (magenta, T9026), and DAPI (blue) in four independent replicates. The scale bar corresponds to 20µm. ...View More

Markus, F., Kannengieβer, A., Näder, P., Atigbire, P., Scholten, A., Vössing, C., ... & Neidhardt, J. (2021). A novel missense variant in the EML1 gene associated with bilateral ribbon-like subcortical heterotopia leads to ciliary defects. Journal of Human Genetics, 66(12), 1159-1167.

Mouse Anti-EML1 Recombinant Antibody (CBFYE-0827) (V2LY-0825-LY170) in WB

A WB analyses of EML1 protein isolated from fibroblasts derived from the RSH-affected patient (P) and unrelated controls (C1, C2; independent replicates: 3). GAPDH served as a loading control. B Overexpression of wild-type EML1231Gly and mutated EML1231Asp in HEK-293T. A mock control (empty vector), a control for transfection reagent (PEI), and wild-type protein form HEK-293T cells were used as controls. Using whole-cell lysate EML1 protein was precipitated by EML1 antibody and WB analyses were performed with EML1 and α-tubulin antibodies. ...View More

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0827

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 60-120 mapping within an internal region of human EML1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Echinoderm Microtubule Associated Protein Like 1

Entrez Gene ID

2009

UniProt ID

O00423

Research Area

Modulates the assembly and organization of the microtubule cytoskeleton, and probably plays a role in regulating the orientation of the mitotic spindle and the orientation of the plane of cell division. Required for normal proliferation of neuronal progenitor cells in the developing brain and for normal brain development. Does not affect neuron migration per se.

Biological Process

Brain development Source: UniProtKB
Hematopoietic progenitor cell differentiation Source: Ensembl
Microtubule cytoskeleton organization Source: UniProtKB
Mitotic spindle organization Source: UniProtKB
Neuroblast proliferation Source: UniProtKB

Cellular Location

Cytoskeleton; Cytoplasm; Perinuclear region. Detected in cytoplasmic punctae. Co-localizes with microtubules (PubMed:24859200). Enriched in perinuclear regions during interphase and in the region of spindle microtubules during metaphase. Enriched at the midzone during telophase and cytokinesis. Detected at growth cones in neurons (By similarity).

Involvement in disease

Band heterotopia (BH):
A brain malformation of the lissencephaly spectrum, resulting from disordered neuronal migration and characterized by bands of gray matter interposed in the central white matter. Disease features include severe developmental delay with intellectual disability, enlarged head circumference, periventricular and ribbon-like subcortical heterotopia, polymicrogyria and agenesis of the corpus callosum.

Markus, F., Kannengießer, A., Näder, P., Atigbire, P., Scholten, A., Vössing, C., ... & Neidhardt, J. (2021). A novel missense variant in the EML1 gene associated with bilateral ribbon-like subcortical heterotopia leads to ciliary defects. Journal of human genetics, 66(12), 1159-1167.

Collin, G. B., Won, J., Krebs, M. P., Hicks, W. J., Charette, J. R., Naggert, J. K., & Nishina, P. M. (2020). Disruption in murine Eml1 perturbs retinal lamination during early development. Scientific reports, 10(1), 1-13.

Oegema, R., McGillivray, G., Leventer, R., Le Moing, A. G., Bahi‐Buisson, N., Barnicoat, A., ... & Lequin, M. H. (2019, December). EML1‐associated brain overgrowth syndrome with ribbon‐like heterotopia. In American Journal of Medical Genetics Part C: Seminars in Medical Genetics (Vol. 181, No. 4, pp. 627-637). Hoboken, USA: John Wiley & Sons, Inc..

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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