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Mouse Anti-EMX1 Recombinant Antibody (CBFYE-0836) (CBMAB-E1291-FY)

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Published Data
CBMAB-E1291-FY-1
Mouse Anti-EMX1 Recombinant Antibody (CBFYE-0836) (CBMAB-E1291-FY) in WB
Expression of EMX1 protein in different grades of SCG. ...View More
Han, Z., Mou, Z., Jing, Y., Jiang, R., & Sun, T. (2022). EMX1 functions as a tumor inhibitor in spinal cord glioma through transcriptional suppression of WASF2 and inactivation of the Wnt/β-catenin axis. Brain and Behavior, 12(8), e2684.
CBMAB-E1291-FY-2
Mouse Anti-EMX1 Recombinant Antibody (CBFYE-0836) (CBMAB-E1291-FY) in IF
DNA replication ability of the SCG cells determined by the EdU labeling assay. ...View More
Han, Z., Mou, Z., Jing, Y., Jiang, R., & Sun, T. (2022). EMX1 functions as a tumor inhibitor in spinal cord glioma through transcriptional suppression of WASF2 and inactivation of the Wnt/β-catenin axis. Brain and Behavior, 12(8), e2684.
Datasheet
SDS
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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal
Mouse
Clone
CBFYE-0836
Application
WB, IP, IF, ELISA
Immunogen
Amino acids 1-50 mapping at the N-terminus of human Emx1.
Host Species
Mouse
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1, κ
Clonality
Monoclonal Antibody
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.
ApplicationNote
ELISA1:100-1:1,000
WB1:100-1:1,000
IP1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, gelatin
Preservative
Sodium azide
Concentration
0.2 mg/ml
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.
More Infomation

Target

Full Name
Empty Spiracles Homeobox 1
Introduction
EMX1 (Empty Spiracles Homeobox 1) is a Protein Coding gene. Diseases associated with EMX1 include Kallmann Syndrome. Gene Ontology (GO) annotations related to this gene include sequence-specific DNA binding. An important paralog of this gene is EMX2.
Entrez Gene ID
Human2016
Mouse13796
Rat500235
UniProt ID
HumanQ04741
MouseQ04742
RatD3ZV90
Alternative Names
Empty Spiracles Homeobox 1; Empty Spiracles Homolog 1 (Drosophila); Empty Spiracles-Like Protein 1; Empty Spiracles Homolog 1
Research Area
Transcription factor, which in cooperation with EMX2, acts to generate the boundary between the roof and archipallium in the developing brain. May function in combinations with OTX1/2 to specify cell fates in the developing central nervous system.
Biological Process
Brain development Source: GO_Central
Brain morphogenesis Source: Ensembl
Central nervous system development Source: GO_Central
Cerebral cortex neuron differentiation Source: Ensembl
Cerebral cortex regionalization Source: Ensembl
Homeostasis of number of cells Source: Ensembl
In utero embryonic development Source: Ensembl
Neuroepithelial cell differentiation Source: Ensembl
Neuron differentiation Source: GO_Central
Neuron projection extension Source: Ensembl
Post-embryonic development Source: Ensembl
Radial glial cell differentiation Source: Ensembl
Regulation of oligodendrocyte progenitor proliferation Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Response to drug Source: Ensembl
Cellular Location
Nucleus; Cytoplasm. Might be shuttling between the nucleus and the cytoplasm.

Han, Z., Mou, Z., Jing, Y., Jiang, R., & Sun, T. (2022). EMX1 functions as a tumor inhibitor in spinal cord glioma through transcriptional suppression of WASF2 and inactivation of the Wnt/β‐catenin axis. Brain and Behavior, 12(8), e2684.

Jimenez-García, M. P., Lucena-Cacace, A., Otero-Albiol, D., & Carnero, A. (2021). Empty spiracles homeobox genes EMX1 and EMX2 regulate WNT pathway activation in sarcomagenesis. Journal of Experimental & Clinical Cancer Research, 40(1), 1-18.

Jimenez-García, M. P., Lucena-Cacace, A., Otero-Albiol, D., & Carnero, A. (2021). Regulation of sarcomagenesis by the empty spiracles homeobox genes EMX1 and EMX2. Cell death & disease, 12(6), 1-17.

Morales, E. E., Handa, N., Drummond, B. E., Chambers, J. M., Marra, A. N., Addiego, A., & Wingert, R. A. (2018). Homeogene emx1 is required for nephron distal segment development in zebrafish. Scientific reports, 8(1), 1-17.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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