Mouse Anti-ENO2 Recombinant Antibody (CBT65) (V2LY-0625-LY3829)

Name: Mouse Anti-ENO2 Recombinant Antibody (CBT65)
SKU: V2LY-0625-LY3829
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-ENO2 Recombinant Antibody (CBT65) (V2LY-0625-LY3829) in IF

Mouse Anti-ENO2 Recombinant Antibody (CBT65) (V2LY-0625-LY3829) in IHC

Mouse Anti-ENO2 Recombinant Antibody (CBT65) (V2LY-0625-LY3829) in WB

Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBT65

Application

WB, IF, ICC, IHC-P

Immunogen

Synthetic Peptide of NSE

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IF	1:200
IHC	1:200
WB	1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, Glycerol, BSA

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Enolase 2

Entrez Gene ID

2026

UniProt ID

P09104

Alternative Names

Enolase 2; 2-Phospho-D-Glycerate Hydro-Lyase; Enolase 2 (Gamma, Neuronal); Neuronal Enriched Enolase; Neuron-Specific Enolase; Neural Enolase; Gamma-Enolase; EC 4.2.1.11;

Research Area

Has neurotrophic and neuroprotective properties on a broad spectrum of central nervous system (CNS) neurons. Binds, in a calcium-dependent manner, to cultured neocortical neurons and promotes cell survival (By similarity).

Biological Process

Glycolytic process Source: CAFA
Regulation of vacuole fusion, non-autophagic Source: GO_Central

Cellular Location

Cell membrane; Cytoplasm. Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form.

Yukimoto, R., Nishida, N., Hata, T., Fujino, S., Ogino, T., Miyoshi, N., ... & Eguchi, H. (2021). Specific activation of glycolytic enzyme enolase 2 in BRAF V600E‐mutated colorectal cancer. Cancer Science, 112(7), 2884-2894.

Sun, C., Liu, M., Zhang, W., Wang, S., Qian, G., Wang, M., & Zhang, G. (2021). Overexpression of enolase 2 is associated with worsened prognosis and increased glycikolysis in papillary renal cell carcinoma. Journal of Cellular Physiology, 236(5), 3821-3831.

Zheng, Y., Wu, C., Yang, J., Zhao, Y., Jia, H., Xue, M., ... & Dong, Q. (2020). Insulin-like growth factor 1-induced enolase 2 deacetylation by HDAC3 promotes metastasis of pancreatic cancer. Signal transduction and targeted therapy, 5(1), 1-14.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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