Mouse Anti-ENOPH1 Recombinant Antibody (CBFYE-0218) (CBMAB-E0467-FY)

Name: Mouse Anti-ENOPH1 Recombinant Antibody (CBFYE-0218)
SKU: CBMAB-E0467-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-0218

Application

ELISA, IF, IHC-P, WB, IP

Immunogen

Full length of human ENOPH1.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
ELISA	1:100-1:1,000
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Epitope

AA 1-116

More Infomation

Target

Full Name

ENOLASE-PHOSPHATASE 1

Introduction

ENOPH1 (Enolase-Phosphatase 1) is a Protein Coding gene. Diseases associated with ENOPH1 include Chromosome 4Q21 Deletion Syndrome. Among its related pathways are CDK-mediated phosphorylation and removal of Cdc6 and Sulfur amino acid metabolism. Gene Ontology (GO) annotations related to this gene include hydrolase activity and acireductone synthase activity.

Entrez Gene ID

58478

UniProt ID

Q9UHY7

Alternative Names

Enolase-Phosphatase 1; 2,3-Diketo-5-Methylthio-1-Phosphopentane Phosphatase; Enolase-Phosphatase E1; Acireductone Synthase; EC 3.1.3.77; MASA

Research Area

Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene).

Biological Process

L-methionine salvage from methylthioadenosine Source: UniProtKB
L-methionine salvage from S-adenosylmethionine Source: UniProtKB-UniRule

Cellular Location

Nucleus; Cytoplasm

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-ENOPH1 Recombinant Antibody (3C1-1D5) (CAT#: V2LY-0825-LY273)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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