Mouse Anti-EphB3 Recombinant Antibody (CBT3563) (V2LY-0625-LY2149)

Name: Mouse Anti-EphB3 Recombinant Antibody (CBT3563)
SKU: V2LY-0625-LY2149
Rating: 5 (1 reviews)

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Tested Data

Mouse Anti-EphB3 Recombinant Antibody (CBT3563) (V2LY-0625-LY2149) in WB

Mouse Anti-EphB3 Recombinant Antibody (CBT3563) (V2LY-0625-LY2149) in IHC

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBT3563

Application

IHC

Immunogen

Purified recombinant fragment of EphB3 (aa39-212) expressed in E. Coli.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:2,000
IHC-P	1:200-1:1,000
ELISA	1:10,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Supernatant

Preservative

Sodium azide

Concentration

Batch dependent

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

EPH Receptor B3

Entrez Gene ID

2049

UniProt ID

P54753

Research Area

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt.

Biological Process

Angiogenesis Source: UniProtKB
Axonal fasciculation Source: UniProtKB
Axon guidance Source: UniProtKB
Cell migration Source: UniProtKB
Central nervous system projection neuron axonogenesis Source: Ensembl
Corpus callosum development Source: UniProtKB
Dendritic spine development Source: UniProtKB
Dendritic spine morphogenesis Source: UniProtKB
Digestive tract morphogenesis Source: UniProtKB
Ephrin receptor signaling pathway Source: UniProtKB
Positive regulation of kinase activity Source: GO_Central
Positive regulation of synapse assembly Source: UniProtKB
Protein autophosphorylation Source: UniProtKB
Regulation of axonogenesis Source: UniProtKB
Regulation of cell-cell adhesion Source: UniProtKB
Regulation of GTPase activity Source: UniProtKB
Retinal ganglion cell axon guidance Source: Ensembl
Roof of mouth development Source: UniProtKB
Substrate adhesion-dependent cell spreading Source: UniProtKB
Thymus development Source: UniProtKB
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central
Urogenital system development Source: UniProtKB

Cellular Location

Cell membrane; Dendrite

Topology

Extracellular: 34-559
Helical: 560-580
Cytoplasmic: 581-998

PTM

Phosphorylated. Autophosphorylates upon ligand-binding. Autophosphorylation on Tyr-614 is required for interaction with SH2 domain-containing proteins.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Antibody Pairs

EPHB3 Matched Antibody Pair (385) (CAT#: APMAB-385LY)

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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