Rabbit Anti-EPS15 Recombinant Antibody (DR) (CBMAB-E1609-FY)

Name: Rabbit Anti-EPS15 Recombinant Antibody (DR)
SKU: CBMAB-E1609-FY
Rating: 5 (1 reviews)

See all EPS15 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

Application

WB, FC, IF

Immunogen

E.coli-derived recombinant human Eps15 (Ala448-Thr579).

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1 µg/ml
FC	0.25 µg/10^6 cells
IF(ICC)	1-25 µg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS, trehalose

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Epidermal Growth Factor Receptor Pathway Substrate 15

Introduction

This gene encodes a protein that is part of the EGFR pathway. The protein is present at clatherin-coated pits and is involved in receptor-mediated endocytosis of EGF. Notably, this gene is rearranged with the HRX/ALL/MLL gene in acute myelogeneous leukemias. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Entrez Gene ID

37961

UniProt ID

Q9W111

Alternative Names

Epidermal Growth Factor Receptor Pathway Substrate 15; Protein AF-1p; AF1P; Epidermal Growth Factor Receptor Substrate 15; ALL1 Fused Gene From Chromosome 1; Protein Eps15; AF-1P; MLLT5

Research Area

Involved in cell growth regulation. May be involved in the regulation of mitogenic signals and control of cell proliferation. Involved in the internalization of ligand-inducible receptors of the receptor tyrosine kinase (RTK) type, in particular EGFR. Plays a role in the assembly of clathrin-coated pits (CCPs). Acts as a clathrin adapter required for post-Golgi trafficking. Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR); internalization of ITGB1 as DAB2-dependent cargo but not TFR seems to require association with DAB2.

Biological Process

Clathrin coat assembly Source: BHF-UCL
Endocytic recycling Source: BHF-UCL
Endocytosis Source: GO_Central
Endosomal transport Source: GO_Central
Golgi to endosome transport Source: UniProtKB
Positive regulation of receptor recycling Source: Ensembl
Postsynaptic neurotransmitter receptor internalization Source: Ensembl
Protein transport Source: UniProtKB-KW
Receptor-mediated endocytosis of virus by host cell Source: CACAO
Regulation of cell population proliferation Source: Ensembl
Vesicle organization Source: UniProtKB
Viral entry into host cell Source: CACAO

Cellular Location

Cytoplasm; Cell membrane; Clathrin-coated pit. Recruited to the plasma membrane upon EGFR activation and localizes to coated pits. Colocalizes with UBQLN1 in ubiquitin-rich cytoplasmic aggregates that are not endocytic compartments and in cytoplasmic juxtanuclear structures called aggresomes.
Isoform 2: Early endosome membrane. Colocalizes with HGS on bilayered clathrin coats on endosomes.

Involvement in disease

A chromosomal aberration involving EPS15 is found in acute leukemias. Translocation t(1;11)(p32;q23) with KMT2A/MLL1. The result is a rogue activator protein.

PTM

Phosphorylation on Tyr-849 is involved in the internalization of EGFR. Not required for membrane translocation after EGF treatment or for targeting to coated pits, but essential for a subsequent step in EGFR endocytosis (By similarity). Phosphorylated on serine upon DNA damage, probably by ATM or ATR.By similarity
Ubiquitinated.By similarity

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Rabbit Anti-EPS15 Recombinant Antibody (DR)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-Eps15 Recombinant Antibody (2) (CAT#: CBMAB-1209CQ)

Mouse Anti-EPS15 Recombinant Antibody (15) (CAT#: CBMAB-XB0404-YC)

Mouse Anti-EPS15 Recombinant Antibody (CBFYE-1078) (CAT#: V2LY-0825-LY557)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry