Mouse Anti-ERBIN Recombinant Antibody (CBFYE-1142) (CBMAB-E1701-FY)

Name: Mouse Anti-ERBIN Recombinant Antibody (CBFYE-1142)
SKU: CBMAB-E1701-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYE-1142

Application

ELISA, WB, IP

Immunogen

Amino acids 1272-1371 representing partial length human ERBIN.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Epitope

AA 1272-1371

More Infomation

Target

Full Name

Erbb2 Interacting Protein

Introduction

This gene is a member of the leucine-rich repeat and PDZ domain (LAP) family. The encoded protein contains 17 leucine-rich repeats and one PDZ domain. It binds to the unphosphorylated form of the ERBB2 protein and regulates ERBB2 function and localization. It has also been shown to affect the Ras signaling pathway by disrupting Ras-Raf interaction. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Entrez Gene ID

55914

UniProt ID

Q96RT1

Alternative Names

Erbb2 Interacting Protein; Densin-180-Like Protein; ERBB2-Interacting Protein; Protein LAP2; ERBB2IP; LAP2

Research Area

Acts as an adapter for the receptor ERBB2, in epithelia. By binding the unphosphorylated 'Tyr-1248' of receptor ERBB2, it may contribute to stabilize this unphosphorylated state (PubMed:16203728).

Inhibits NOD2-dependent NF-kappa-B signaling and proinflammatory cytokine secretion (PubMed:16203728).

Biological Process

Basal protein localization Source: UniProtKB
Cell adhesion Source: UniProtKB
Cellular response to tumor necrosis factor Source: UniProtKB
Epidermal growth factor receptor signaling pathway Source: UniProtKB
Establishment or maintenance of epithelial cell apical/basal polarity Source: UniProtKB
Integrin-mediated signaling pathway Source: UniProtKB
Intermediate filament cytoskeleton organization Source: UniProtKB
Negative regulation of monocyte chemotactic protein-1 production Source: Ensembl
Negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
Negative regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway Source: UniProtKB
Positive regulation of Ras protein signal transduction Source: GO_Central
Protein targeting Source: Ensembl
Regulation of postsynaptic membrane neurotransmitter receptor levels Source: Ensembl
Response to lipopolysaccharide Source: UniProtKB
Response to muramyl dipeptide Source: UniProtKB
Signal transduction Source: GO_Central

Cellular Location

Nucleus membrane; Basolateral cell membrane; Hemidesmosome. Found in hemidesmosomes, which are cell-substrate adhesion complexes in stratified epithelia. In transfected cells, either diffusely distributed over the cytoplasm or concentrated at the basolateral membrane. Colocalizes with the adrenergic receptors, ADREN1A and ADREN1B, at the nuclear membrane of cardiac myocytes (By similarity).

Şahin, F. (2022). Analysis of the effect of Erbin on the epithelial to mesenchymal transition related genes in breast cancer (Doctoral dissertation, Bilkent University).

Jang, H., Stevens, P., Gao, T., & Galperin, E. (2021). The leucine‐rich repeat signaling scaffolds Shoc2 and Erbin: cellular mechanism and role in disease. The FEBS journal, 288(3), 721-739.

Tan, Y., Lin, X. T., Luo, Y. D., Zhang, J., Fang, L., Zhu, Y. Y., ... & Xie, C. M. (2020). Reduced IκBα promotes hepatocellular carcinoma cell proliferation and migration via regulation of NF‑κB/Erbin axis. Oncology Letters, 20(5), 1-1.

Luo, Z. Y., Huang, L., Lin, S., Yin, Y. N., Jie, W., Hu, N. Y., ... & Gao, T. M. (2020). Erbin in amygdala parvalbumin-positive neurons modulates anxiety-like behaviors. Biological psychiatry, 87(10), 926-936.

Jin, X., Li, B., Zhao, Y., Liu, X., Li, Y., Song, L., ... & Guo, Y. (2019). Erbin plays a critical role in human umbilical vein endothelial cell migration and tubular structure formation via the Smad1/5 pathway. Journal of Cellular Biochemistry, 120(3), 4654-4664.

Valenzuela-Iglesias, A., Burks, H. E., Arnette, C. R., Yalamanchili, A., Nekrasova, O., Godsel, L. M., & Green, K. J. (2019). Desmoglein 1 Regulates Invadopodia by Suppressing EGFR/Erk Signaling in an Erbin-Dependent MannerDesmoglein 1 Suppresses Invadopodia and Matrix Degradation. Molecular Cancer Research, 17(5), 1195-1206.

Zhao, Y., Zhang, X., Wang, R., Bing, J., Wu, F., Zhang, Y., ... & Zeng, S. (2018). Erbin and ErbB2 play roles in the sexual differentiation of the song system nucleus HVC in bengalese finches (Lonchura Striata var. domestica). Developmental Neurobiology, 78(1), 15-38.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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For research use only. Not intended for any clinical use.

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