Mouse Anti-ERGIC3 Recombinant Antibody (EG1137) (V2LY-0825-LY708)

Name: Mouse Anti-ERGIC3 Recombinant Antibody (EG1137)
SKU: V2LY-0825-LY708
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

EG1137

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 171-194 within an internal region of human ERGIC-3.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2b, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Ergic And Golgi 3

Entrez Gene ID

Human	51614
Mouse	66366
Rat	296306

UniProt ID

Human	Q9Y282
Mouse	Q9CQE7
Rat	D3ZU83

Research Area

Possible role in transport between endoplasmic reticulum and Golgi.

Biological Process

Endoplasmic reticulum to Golgi vesicle-mediated transport Source: GO_Central
Retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum Source: GO_Central

Cellular Location

Cis-Golgi network membrane; Endoplasmic reticulum membrane; Endoplasmic reticulum-Golgi intermediate compartment membrane. Cycles between the endoplasmic reticulum and the Golgi.

Topology

Cytoplasmic: 1-25
Helical: 26-46
Lumenal: 47-341
Helical: 342-362
Cytoplasmic: 363-383

Guan, L., Yang, Y., Liang, J., Miao, Y., Shang, A., Wang, B., ... & Ding, M. (2022). ERGIC2 and ERGIC3 regulate the ER‐to‐Golgi transport of gap junction proteins in metazoans. Traffic, 23(3), 140-157.

Liu, M., Zhao, Q., Zheng, X., Yang, L., Zhao, Y., Li, X., & Wu, M. (2022). Transcriptome changes in ERGIC3-knockdown hepatocellular carcinoma cells: ERGIC3 is a novel immune function related gene. PeerJ, 10, e13369.

Zhao, Q., Wu, M., Zheng, X., Yang, L., Zhang, Z., Li, X., & Chen, J. (2020). ERGIC3 silencing additively enhances the growth inhibition of BFA on lung adenocarcinoma cells. Current Cancer Drug Targets, 20(1), 67-75.

Yoo, W., Cho, E. B., Kim, S., & Yoon, J. B. (2019). The E3 ubiquitin ligase MARCH2 regulates ERGIC3-dependent trafficking of secretory proteins. Journal of Biological Chemistry, 294(28), 10900-10912.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-ERGIC3 Recombinant Antibody (CBFYE-1191) (CAT#: V2LY-0825-LY707)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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