Mouse Anti-ESPL1 Recombinant Antibody (6H6) (V2LY-0825-LY769)

Name: Mouse Anti-ESPL1 Recombinant Antibody (6H6)
SKU: V2LY-0825-LY769
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

6H6

Application

WB, ELISA, IHC

Immunogen

ESPL1 (aa 586-701) partial recombinant protein.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC	3 μg/ml

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

extra spindle pole bodies homolog 1 (S. cerevisiae)

Entrez Gene ID

9700

UniProt ID

Q14674

Research Area

Caspase-like protease, which plays a central role in the chromosome segregation by cleaving the SCC1/RAD21 subunit of the cohesin complex at the onset of anaphase. During most of the cell cycle, it is inactivated by different mechanisms.

Biological Process

Apoptotic process Source: UniProtKB
Establishment of mitotic spindle localization Source: UniProtKB
Homologous chromosome segregation Source: Ensembl
Meiotic chromosome separation Source: GO_Central
Meiotic spindle organization Source: Ensembl
Mitotic cytokinesis Source: UniProtKB
Mitotic sister chromatid segregation Source: UniProtKB
Negative regulation of sister chromatid cohesion Source: UniProtKB
Positive regulation of mitotic metaphase/anaphase transition Source: UniProtKB

Cellular Location

Nucleus; Cytoplasm

PTM

Autocleaves. This function, which is not essential for its protease activity, is unknown.
Phosphorylated by CDK1. There are 8 Ser/Thr phosphorylation sites. Among them, Ser-1126 phosphorylation is the major site, which conducts to the enzyme inactivation.

Liu, X., Zeng, W., Zheng, D., Tang, M., & Zhou, W. (2022). Let-7c-5p Restrains Cell Growth and Induces Apoptosis of Lung Adenocarcinoma Cells via Targeting ESPL1. Molecular Biotechnology, 1-9.

Rongming Wang, M. D., Weiwei Zang, M. D., Bobin Hu, M. D., Deli Deng, M. D., Xiaozhang Ling, M. D., & Huikun Zhou, M. D. (2020). Serum ESPL1 Can Be Used as a Biomarker for Patients With Hepatitis B Virus-Related Liver Cancer: A Chinese Case-Control Study.

Mamoor, S. (2020). ESPL1 (Separase) is differentially expressed in high-grade serous ovarian cancers.

Hu, B., Huang, W., Wang, R., Zang, W., Su, M., Li, H., ... & Jiang, J. (2020). High Rate of Detection of Human ESPL1-HBV S Fusion Gene in Patients With HBV-related Liver Cancer: A Chinese Case–Control Study. Anticancer research, 40(1), 245-252.

Yang, Q., Yu, B., & Sun, J. (2020). TTK, CDC25A, and ESPL1 as prognostic biomarkers for endometrial cancer. BioMed Research International, 2020.

Wang, R., Zang, W., Hu, B., Deng, D., Ling, X., Zhou, H., ... & Jiang, J. (2020). Serum ESPL1 Can Be Used as a Biomarker for Patients With Hepatitis B Virus-Related Liver Cancer: A Chinese Case-Control Study. Technology in Cancer Research & Treatment, 19, 1533033820980785.

Deng, J., Cheng, X., & Zhou, Y. (2019, August). AGRP and ESPL1 as Biomarkers of Brain-Metastasis in Lung Adenocarcinoma. In Journal of Physics: Conference Series (Vol. 1302, No. 4, p. 042040). IOP Publishing.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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