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Mouse Anti-ESRRB Recombinant Antibody (H6707) (CBMAB-E1909-FY)

This product is mouse antibody that recognizes ESRRB. The antibody H6707 can be used for immunoassay techniques such as: WB, IP, ELISA.
See all ESRRB antibodies
Published Data
Fig.1 Western blotting showing Ncoa3 down-regulation along with Esrrb, Nanog, and Oct4 upon LIF withdrawal.
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Fig.2 Western blotting showing specific Esrrb protein depletion 48 h after transfection with shEsrrb.
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Fig.3 Ncoa3 protein is up-regulated in fully reprogrammed iPSC lines.
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Fig.4 Co-IP experiments using antibodies against Oct4, Esrrb, or control immunoglobulin G (IgG) confirm the Oct4-Esrrb interaction.
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Fig.5 Immunofluorescence of ERRb in LNCaP-ERRb clones.
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Summary

Host Animal
Mouse
Specificity
Human
Clone
H6707
Antibody Isotype
IgG2a
Application
WB, IP, ELISA

Basic Information

Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
ESRRB
Introduction
This gene encodes a protein with similarity to the estrogen receptor. Its function is unknown; however, a similar protein in mouse plays an essential role in placental development.
Entrez Gene ID
2103
UniProt ID
O95718
Alternative Names
Estrogen Related Receptor Beta; Nuclear Receptor Subfamily 3 Group B Member 2; Estrogen Receptor-Like 2; ERR Beta-2; ERR-Beta; ESRL2; NR3B2; Estrogen-Related Nuclear Receptor Beta; Deafness, Autosomal Recessive 35; Estrogen-Related Receptor Beta
Research Area
Isoform 3:
Transcription factor that binds a canonical ESRRB recognition (ERRE) sequence 5'TCAAGGTCA-3' localized on promoter and enhancer of targets genes regulating their expression or their transcription activity (PubMed:17920186, PubMed:19755138).

Plays a role, in a LIF-independent manner, in maintainance of self-renewal and pluripotency of embryonic and trophoblast stem cells through different signaling pathways including FGF signaling pathway and Wnt signaling pathways. Upon FGF signaling pathway activation, interacts with KDM1A by directly binding to enhancer site of ELF5 and EOMES and activating their transcription leading to self-renewal of trophoblast stem cells. Also regulates expression of multiple rod-specific genes and is required for survival of this cell type (By similarity).

Plays a role as transcription factor activator of GATA6, NR0B1, POU5F1 and PERM1 (PubMed:23836911).

Plays a role as transcription factor repressor of NFE2L2 transcriptional activity and ESR1 transcriptional activity (PubMed:17920186, PubMed:19755138).

During mitosis remains bound to a subset of interphase target genes, including pluripotency regulators, through the canonical ESRRB recognition (ERRE) sequence, leading to their transcriptional activation in early G1 phase. Can coassemble on structured DNA elements with other transcription factors like SOX2, POU5F1, KDM1A and NCOA3 to trigger ESRRB-dependent gene activation. This mechanism, in the case of SOX2 corecruitment prevents the embryonic stem cells (ESCs) to epiblast stem cells (EpiSC) transition through positive regulation of NR0B1 that inhibits the EpiSC transcriptional program. Also plays a role inner ear development by controlling expression of ion channels and transporters and in early placentation (By similarity).

Isoform 1:
Transcription factor that binds a canonical ESRRB recognition (ERRE) sequence 5'TCAAGGTCA-3' localized on promoter and enhancer of targets genes regulating their expression or their transcription activity. Positively regulates ESR1 transcriptional activity upon E2 stimulation.
Biological Process
Cell dedifferentiation Source: UniProtKB
Cell population proliferation Source: UniProtKB
Inner ear development Source: UniProtKB
Negative regulation of stem cell differentiation Source: UniProtKB
Photoreceptor cell maintenance Source: UniProtKB
Positive regulation of stem cell population maintenance Source: UniProtKB
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Positive regulation of transcription involved in G1/S transition of mitotic cell cycle Source: UniProtKB
Positive regulation of transcription involved in G2/M transition of mitotic cell cycle Source: UniProtKB
Regulation of stem cell division Source: UniProtKB
Regulation of transcription, DNA-templated Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Stem cell division Source: UniProtKB
Stem cell population maintenance Source: UniProtKB
Cellular Location
Nucleus; Cytoplasm; Chromosome
Involvement in disease
Deafness, autosomal recessive, 35 (DFNB35):
A form of non-syndromic deafness characterized by non-progressive, prelingual hearing loss.
PTM
Acetylated by PCAF/KAT2 (in vitro).

Levy, S. H., Cohen, S. F., Arnon, L., Lahav, S., Awawdy, M., Alajem, A., ... & Ram, O. (2022). Esrrb is a cell-cycle-dependent associated factor balancing pluripotency and XEN differentiation. Stem Cell Reports.

Elbagoury, N. M., Ashaat, E. A., Sharaf-Eldin, W. E., Hassib, N., Ashaat, N. A., Youssef, R., ... & Esswai, M. L. (2022). Expanding the phenotypic spectrum in ESRRB related sensorineural hearing loss: Evidence provided via detecting novel nonsense mutation in an Egyptian family. Human Gene, 34, 201105.

Festuccia, N., Owens, N., Chervova, A., Dubois, A., & Navarro, P. (2021). The combined action of Esrrb and Nr5a2 is essential for murine naïve pluripotency. Development, 148(17), dev199604.

Yu, S., Zhang, R., Shen, Q., Zhu, Z., Zhang, J., Wu, X., ... & Hua, J. (2021). ESRRB Facilitates the Conversion of Trophoblast-Like Stem Cells From Induced Pluripotent Stem Cells by Directly Regulating CDX2. Frontiers in Cell and Developmental Biology, 9.

Fan, R., Kim, Y. S., Wu, J., Chen, R., Zeuschner, D., Mildner, K., ... & Bedzhov, I. (2020). Wnt/Beta-catenin/Esrrb signalling controls the tissue-scale reorganization and maintenance of the pluripotent lineage during murine embryonic diapause. Nature communications, 11(1), 1-17.

Okamura, E., Tam, O. H., Posfai, E., Li, L., Cockburn, K., Lee, C. Q., ... & Rossant, J. (2019). Esrrb function is required for proper primordial germ cell development in presomite stage mouse embryos. Developmental biology, 455(2), 382-392.

Hao, Y., Fan, X., Shi, Y., Zhang, C., Sun, D. E., Qin, K., ... & Chen, X. (2019). Next-generation unnatural monosaccharides reveal that ESRRB O-GlcNAcylation regulates pluripotency of mouse embryonic stem cells. Nature communications, 10(1), 1-13.

Festuccia, N., Owens, N., & Navarro, P. (2018). Esrrb, an estrogen‐related receptor involved in early development, pluripotency, and reprogramming. FEBS letters, 592(6), 852-877.

Adachi, K., Kopp, W., Wu, G., Heising, S., Greber, B., Stehling, M., ... & Schöler, H. R. (2018). Esrrb unlocks silenced enhancers for reprogramming to naive pluripotency. Cell stem cell, 23(2), 266-275.

Festuccia, N., Halbritter, F., Corsinotti, A., Gagliardi, A., Colby, D., Tomlinson, S. R., & Chambers, I. (2018). Esrrb extinction triggers dismantling of naïve pluripotency and marks commitment to differentiation. The EMBO journal, 37(21), e95476.

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For research use only. Not intended for any clinical use.

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