Mouse Anti-F12 Recombinant Antibody (CBXF-2379) (CBMAB-F0699-CQ)

Name: Mouse Anti-F12 Recombinant Antibody (CBXF-2379)
SKU: CBMAB-F0699-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXF-2379

Application

WB, ELISA

Immunogen

Human Factor XII.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS

Preservative

None

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Coagulation Factor XII

Introduction

This gene encodes coagulation factor XII which circulates in blood as a zymogen. This single chain zymogen is converted to a two-chain serine protease with an heavy chain (alpha-factor XIIa) and a light chain. The heavy chain contains two fibronectin-type domains, two epidermal growth factor (EGF)-like domains, a kringle domain and a proline-rich domain, whereas the light chain contains only a catalytic domain. On activation, further cleavages takes place in the heavy chain, resulting in the production of beta-factor XIIa light chain and the alpha-factor XIIa light chain becomes beta-factor XIIa heavy chain. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then to beta-factor XIIa. The active factor XIIa participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. It activates coagulation factors VII and XI. Defects in this gene do not cause any clinical symptoms and the sole effect is that whole-blood clotting time is prolonged.

Entrez Gene ID

2161

UniProt ID

P00748

Alternative Names

Coagulation Factor XII; Hageman Factor; EC 3.4.21.38; HAF; Coagulation Factor XII (Hageman Factor); Coagulation Factor XIIa Heavy Chain; Coagulation Factor XIIa Light Chain;

Research Area

Factor XII is a serum glycoprotein that participates in the initiation of blood coagulation, fibrinolysis, and the generation of bradykinin and angiotensin. Prekallikrein is cleaved by factor XII to form kallikrein, which then cleaves factor XII first to alpha-factor XIIa and then trypsin cleaves it to beta-factor XIIa. Alpha-factor XIIa activates factor XI to factor XIa.

Biological Process

Blood coagulation Source: GO_Central
Blood coagulation, intrinsic pathway Source: BHF-UCL
Factor XII activation Source: BHF-UCL
Fibrinolysis Source: UniProtKB-KW
Innate immune response Source: BHF-UCL
Plasma kallikrein-kinin cascade Source: BHF-UCL
Positive regulation of blood coagulation Source: BHF-UCL
Positive regulation of fibrinolysis Source: BHF-UCL
Positive regulation of plasminogen activation Source: BHF-UCL
Protein autoprocessing Source: BHF-UCL
Protein processing Source: BHF-UCL
Proteolysis Source: GO_Central
Response to misfolded protein Source: BHF-UCL
Zymogen activation Source: BHF-UCL

Cellular Location

Secreted

Involvement in disease

Factor XII deficiency (FA12D):
An asymptomatic anomaly of in vitro blood coagulation. Its diagnosis is based on finding a low plasma activity of the factor in coagulating assays. It is usually only accidentally discovered through pre-operative blood tests. Factor XII deficiency is divided into two categories, a cross-reacting material (CRM)-negative group (negative F12 antigen detection) and a CRM-positive group (positive F12 antigen detection).
Hereditary angioedema 3 (HAE3):
A hereditary angioedema occurring only in women. Hereditary angioedema is an autosomal dominant disorder characterized by episodic local swelling involving subcutaneous or submucous tissue of the upper respiratory and gastrointestinal tracts, face, extremities, and genitalia. Hereditary angioedema type 3 differs from types 1 and 2 in that both concentration and function of C1 esterase inhibitor are normal. Hereditary angioedema type 3 is precipitated or worsened by high estrogen levels (e.g., during pregnancy or treatment with oral contraceptives).

PTM

Factor XII is activated by kallikrein in alpha-factor XIIa, which is further converted by trypsin into beta-factor XIIa. Alpha-factor XIIa is composed of an NH2-terminal heavy chain, called coagulation factor XIIa heavy chain, and a COOH-terminal light chain, called coagulation factor XIIa light chain, connected by a disulfide bond. Beta-factor XIIa is composed of 2 chains linked by a disulfide bond, an N-terminal nonapeptide, called beta-factor XIIa part 1, and coagulation factor XIIa light chain, also known in this context as beta-factor XIIa part 2.
O- and N-glycosylated. The O-linked polysaccharides were not identified, but are probably the mucin type linked to GalNAc.

Mamoor, S. (2022). Differential expression of coagulation factor XII (F12) in cancers of the breast.

Chou, S. C., Lin, C. Y., Lin, H. Y., Pai, C. H., Yu, C. Y., Kuo, S. F., ... & Shen, M. C. (2022). Characterization of congenital factor XII deficiency in Taiwanese patients: identification of one novel and one common mutation. International Journal of Hematology, 1-6.

Mamoor, S. (2020). Factor XII (Hageman factor) is over-expressed in brain metastatic breast cancer.

Cheng, X., Yang, L., Xin, Y., Zhu, L., Su, M., & Hao, X. (2020). Analysis of a pedigree affected with hereditary coagulation factor XII deficiency due to a homozygous 252delAsn deletion of F12 gene. Zhonghua yi xue yi Chuan xue za zhi= Zhonghua Yixue Yichuanxue Zazhi= Chinese Journal of Medical Genetics, 37(7), 755-758.

Liu, J., Cooley, B. C., Akinc, A., Butler, J., & Borodovsky, A. (2020). Knockdown of liver-derived factor XII by GalNAc-siRNA ALN-F12 prevents thrombosis in mice without impacting hemostatic function. Thrombosis research, 196, 200-205.

Xie, H., Zhang, H., Xu, M., Zou, A., Jin, Y., Yang, L., ... & Wang, M. (2020). Identification of compound heterozygous variants of F12 gene in a pedigree affected with inherited coagulation factor XII deficiency. Zhonghua yi xue yi Chuan xue za zhi= Zhonghua Yixue Yichuanxue Zazhi= Chinese Journal of Medical Genetics, 37(5), 519-522.

Maruyama, H., Brooks, M. B., Stablein, A., & Frye, A. (2019). Factor XII deficiency is common in domestic cats and associated with two high frequency F12 mutations. Gene, 706, 6-12.

Liu, J., Qin, J., Borodovsky, A., Racie, T., Castoreno, A., Schlegel, M., ... & Akinc, A. (2019). An investigational RNAi therapeutic targeting Factor XII (ALN-F12) for the treatment of hereditary angioedema. Rna, 25(2), 255-263.

Heestermans, M., de Jong, A., van Tilburg, S., Reitsma, P. H., Versteeg, H. H., Spronk, H. M., & van Vlijmen, B. J. (2019). Use of “C9/11 Mismatch” Control siRNA Reveals Sequence-Related Off-Target Effect on Coagulation of an siRNA Targeting Mouse Coagulation Factor XII. nucleic acid therapeutics, 29(4), 218-223.

Stavrou, E. X., Fang, C., Bane, K. L., Long, A. T., Naudin, C., Kucukal, E., ... & Schmaier, A. H. (2018). Factor XII and uPAR upregulate neutrophil functions to influence wound healing. The Journal of clinical investigation, 128(3), 944-959.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
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For research use only. Not intended for any clinical use.

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