Mouse Anti-FA2H Recombinant Antibody (CBXF-3546) (V2LY-0825-LY1175)

Name: Mouse Anti-FA2H Recombinant Antibody (CBXF-3546)
SKU: V2LY-0825-LY1175
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXF-3546

Application

WB, IF

Immunogen

Full length human recombinant protein of human FA2H.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:1,000
IF(ICC)	1:50-1:100

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol, BSA

Preservative

Sodium azide

Concentration

1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Fatty Acid 2-Hydroxylase

Entrez Gene ID

79152

UniProt ID

Q7L5A8

Research Area

Catalyzes the hydroxylation of free fatty acids at the C-2 position to produce 2-hydroxy fatty acids, which are building blocks of sphingolipids and glycosphingolipids common in neural tissue and epidermis (PubMed:15337768, PubMed:15863841, PubMed:17355976, PubMed:22517924).

FA2H is stereospecific for the production of (R)-2-hydroxy fatty acids (PubMed:22517924).

Plays an essential role in the synthesis of galactosphingolipids of the myelin sheath (By similarity).

Responsible for the synthesis of sphingolipids and glycosphingolipids involved in the formation of epidermal lamellar bodies critical for skin permeability barrier (PubMed:17355976).

Participates in the synthesis of glycosphingolipids and a fraction of type II wax diesters in sebaceous gland, specifically regulating hair follicle homeostasis (By similarity).

Involved in the synthesis of sphingolipids of plasma membrane rafts, controlling lipid raft mobility and trafficking of raft-associated proteins (By similarity).

Biological Process

Central nervous system myelin maintenance Source: Ensembl
Ceramide biosynthetic process Source: UniProtKB
Establishment of skin barrier Source: UniProtKB
Fatty acid biosynthetic process Source: UniProtKB-KW
Fatty acid metabolic process Source: GO_Central
Galactosylceramide biosynthetic process Source: UniProtKB
Glucosylceramide biosynthetic process Source: UniProtKB
Lipid modification Source: Ensembl
Peripheral nervous system myelin maintenance Source: Ensembl
Plasma membrane raft organization Source: UniProtKB
Regulation of cell population proliferation Source: Ensembl
Regulation of hair cycle Source: Ensembl
Sebaceous gland cell differentiation Source: Ensembl
Sphingolipid biosynthetic process Source: Reactome

Cellular Location

Endoplasmic reticulum membrane; Microsome membrane

Involvement in disease

Spastic paraplegia 35, autosomal recessive (SPG35):
A form of spastic paraplegia, a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. SPG35 is a complicated form characterized by childhood onset of gait difficulties. It has a rapid progression and many patients become wheelchair-bound as young adults. Patients manifest cognitive decline associated with leukodystrophy. Other variable neurologic features, such as dystonia, optic atrophy, and seizures may also occur.

Zhou, X., Huang, F., Ma, G., Wei, W., Wu, N., & Liu, Z. (2022). Dysregulated ceramides metabolism by fatty acid 2-hydroxylase exposes a metabolic vulnerability to target cancer metastasis. Signal Transduction and Targeted Therapy, 7(1), 1-14.

Sakai, G., Hirao-Suzuki, M., Koga, T., Kobayashi, T., Kamishikiryo, J., Tanaka, M., ... & Takeda, S. (2022). Perfluorooctanoic acid (PFOA) as a stimulator of estrogen receptor-negative breast cancer MDA-MB-231 cell aggressiveness: Evidence for involvement of fatty acid 2-hydroxylase (FA2H) in the stimulated cell migration. The Journal of Toxicological Sciences, 47(4), 159-168.

Jordans, S., Hardt, R., Becker, I., Winter, D., Wang-Eckhardt, L., & Eckhardt, M. (2022). Age-Dependent Increase in Schmidt-Lanterman Incisures and a Cadm4-Associated Membrane Skeletal Complex in Fatty Acid 2-hydroxylase Deficient Mice: a Mouse Model of Spastic Paraplegia SPG35. Molecular Neurobiology, 1-11.

Kawaguchi, M., Sassa, T., Kidokoro, H., Nakata, T., Kato, K., Muramatsu, H., ... & Natsume, J. (2020). Novel biallelic FA2H mutations in a Japanese boy with fatty acid hydroxylase-associated neurodegeneration. Brain and Development, 42(2), 217-221.

Qi, T., Wu, D., Duan, Z., Chen, C., Qiu, J., & Kang, J. (2020). Overexpression of Fatty Acid 2-Hydroxylase is Associated with an Increased Sensitivity to Cisplatin by Ovarian Cancer and Better Prognoses. Genetic Testing and Molecular Biomarkers, 24(10), 632-640.

Hirao-Suzuki, M., Koga, T., Sakai, G., Kobayashi, T., Ishii, Y., Miyazawa, H., ... & Takeda, S. (2020). Fatty acid 2-hydroxylase (FA2H) as a stimulatory molecule responsible for breast cancer cell migration. Biochemical and Biophysical Research Communications, 531(2), 215-222.

Hirao-Suzuki, M., Takeda, S., Watanabe, K., Takiguchi, M., & Aramaki, H. (2019). Δ9-Tetrahydrocannabinol upregulates fatty acid 2-hydroxylase (FA2H) via PPARα induction: A possible evidence for the cancellation of PPARβ/δ-mediated inhibition of PPARα in MDA-MB-231 cells. Archives of biochemistry and biophysics, 662, 219-225.

Incecik, F., Besen, S., & Bozdogan, S. T. (2018). Hereditary spastic paraplegia type 35 with a novel mutation in fatty acid 2-hydroxylase gene and literature review of the clinical features. Annals of Indian Academy of Neurology, 21(4), 335.

Hardt, R., Winter, D., Gieselmann, V., & Eckhardt, M. (2018). Identification of progesterone receptor membrane component-1 as an interaction partner and possible regulator of fatty acid 2-hydroxylase. Biochemical Journal, 475(5), 853-871.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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