Mouse Anti-FARP1 Recombinant Antibody (CBXF-0299) (CBMAB-F0334-CQ)

Name: Mouse Anti-FARP1 Recombinant Antibody (CBXF-0299)
SKU: CBMAB-F0334-CQ
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXF-0299

Application

WB, IP, IF, ELISA, IHC-P

Immunogen

Amino acids 471-549 of human FARP1.

Host Species

Mouse

Specificity

Human

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal Antibody

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
IHC-P	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived)

Introduction

This gene encodes a protein containing a FERM (4.2, exrin, radixin, moesin) domain, a Dbl homology domain, and two pleckstrin homology domains. These domains are found in guanine nucleotide exchange factors and proteins that link the cytoskeleton to the cell membrane. The encoded protein functions in neurons to promote dendritic growth. Alternative splicing results in multiple transcript variants.

Entrez Gene ID

10160

UniProt ID

Q9Y4F1

Alternative Names

FERM, ARH/RhoGEF And Pleckstrin Domain Protein 1; FERM, RhoGEF (ARHGEF) And Pleckstrin Domain Protein 1 (Chondrocyte-Derived); Pleckstrin Homology Domain-Containing Family C Member 2; FERM, RhoGEF And Pleckstrin Domain-Containing Protein 1; Protein Phosphatase 1, Regulatory Subunit 75; Chondrocyte-Derived Ezrin-Like Protein; PH Domain-Containing Family C Member 2;

Research Area

Functions as guanine nucleotide exchange factor for RAC1. May play a role in semaphorin signaling. Plays a role in the assembly and disassembly of dendritic filopodia, the formation of dendritic spines, regulation of dendrite length and ultimately the formation of synapses (By similarity).

Biological Process

Dendrite morphogenesis Source: UniProtKB
Postsynaptic actin cytoskeleton organization Source: Ensembl
Regulation of presynapse assembly Source: Ensembl
Retrograde trans-synaptic signaling by trans-synaptic protein complex Source: Ensembl
Synapse assembly Source: UniProtKB

Cellular Location

Cytosol; Cell membrane; Synapse; Synaptosome; Filopodium; Dendrite; Dendritic spine. Recruited to the cell membrane via interaction with CADM1.

Mamoor, S. (2021). Differential expression of FARP1 in cancers of the breast.

Cooke, M., Kreider-Letterman, G., Baker, M. J., Zhang, S., Sullivan, N. T., Eruslanov, E., ... & Kazanietz, M. G. (2021). FARP1, ARHGEF39, and TIAM2 are essential receptor tyrosine kinase effectors for Rac1-dependent cell motility in human lung adenocarcinoma. Cell reports, 37(5), 109905.

Hirano, T., Shinsato, Y., Tanabe, K., Higa, N., Kamil, M., Kawahara, K., ... & Natsugoe, S. (2020). FARP1 boosts CDC42 activity from integrin αvβ5 signaling and correlates with poor prognosis of advanced gastric cancer. Oncogenesis, 9(2), 1-14.

Cucinotta, F., Ricciardello, A., Turriziani, L., Calabrese, G., Briguglio, M., Boncoddo, M., ... & Persico, A. M. (2020). FARP‐1 deletion is associated with lack of response to autism treatment by early start denver model in a multiplex family. Molecular genetics & genomic medicine, 8(9), e1373.

Chen, Z. H., & Wang, L. H. (2019). FARP1 facilitates cell proliferation through modulating MAPK signaling pathway in cutaneous melanoma. The American Journal of Dermatopathology, 41(12), 908-913.

Kuo, Y. C., He, X., Coleman, A. J., Chen, Y. J., Dasari, P., Liou, J., ... & Zhang, X. (2018). Structural analyses of FERM domain-mediated membrane localization of FARP1. Scientific reports, 8(1), 1-10.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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