Mouse Anti-FAS3 Monoclonal Antibody (7G10) (V2LY-0925-LY122)
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Mouse Anti-FAS3 Monoclonal Antibody (7G10) (V2LY-0925-LY122) in IF
(a) Normal ISNb branching pattern and specific innervation along m6/m7 cleft (solid arrows) as revealed by anti-FasII immunostaining in stage 17 wild-type w1118 embryos. (b) Weak ISNb innervations similar to those observed in miR-8Δ/Δ mutant embryos (broken arrow) and normal innervations (solid arrow) along m6/m7 cleft in the adjacent hemisegments of a loss-of-function nrg14 mutant embryo. Scale bar = 10 μm.
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Lu, C. S., Zhai, B., Mauss, A., Landgraf, M., Gygi, S., & Van Vactor, D. (2014). MicroRNA-8 promotes robust motor axon targeting by coordinate regulation of cell adhesion molecules during synapse development. Philosophical Transactions of the Royal Society B: Biological Sciences, 369(1652), 20130517.
Mouse Anti-FAS3 Monoclonal Antibody (7G10) (V2LY-0925-LY122) in IF
Immunofluorescence staining for Fasciclin 3 (red) in the ectoderm allowed visualization of the progress of dorsal closure in embryos expressing the indicated HA-tagged Slpr transgenes (green) with pnr-Gal4 compared to a control w1118 (Aa) embryo. Expression of SlprAAA (Ac) causes detachment of the dorsal ectoderm anteriorly and closure defects, whereas overexpression of SlprWT, PXAP, or PXEP, promoted closure of the epidermis at the dorsal midline (Ab,d,e). Images are dorsolateral views of stage 16/17 embryos with anterior left. (B) JNK target gene expression, monitored by the puc-lacZ reporter, was upregulated in the pnr domain of the embryonic dorsal ectoderm upon expression of all of the indicated Slpr transgenes (Bb,d,e) except the dominant negative SlprAAA (Bc) compared to a control embryo without a Slpr transgene (Ba). Images are dorsolateral views of stage 14/15 embryos, with anterior left.
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Gonda, R. L., Garlena, R. A., & Stronach, B. (2012). Drosophila heat shock response requires the JNK pathway and phosphorylation of mixed lineage kinase at a conserved serine-proline motif.
Mouse Anti-FAS3 Monoclonal Antibody (7G10) (V2LY-0925-LY122) in IF
Lack of migration in a clone of lis-1G10.14 mutant cells (marked by absence of GFP, green) in a late stage 9 egg chamber. All migratory border cells are mutant, but the polar cells, marked by FasIII (red) staining between them, are not. Genotype hsFLP/+; FRTG13,Lis-1G10.14/FRTG13,ubiquitinGFP. Severely defective migration in a clone of Dhc64C4−19 mutant cells (marked by absence of GFP, green); stage 10 egg chamber. Most border cells are mutant and are in the rear; the polar cells marked by FasIII (red) staining between them, are not mutant. Genotype hsFLP/+; Dhc64C4−19,FRT2A/FRT2A,ubiquitnGFP. Scale bar: 30 µm. Expanded view (3×) in bottom corner of E and F also shows DAPI (blue) to mark all nuclei.
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Yang, N., Inaki, M., Cliffe, A., & Rørth, P. (2012). Microtubules and Lis-1/NudE/dynein regulate invasive cell-on-cell migration in Drosophila. PLoS One, 7(7), e40632.
Mouse Anti-FAS3 Monoclonal Antibody (7G10) (V2LY-0925-LY122) in IF
Traffic Jam (TJ)-positive cells (white) were counted in ovaries stained also for Vasa (green), Lamin-C and Fas3 (both in red), and DAPI (blue). Bar, 20 μm applies to all images. (A-C) Up to 36 hr after puparium formation (APF), prior to budding of the first egg chamber, intermingled cells (ICs) (red brackets), defined as intermingled with germline cells (green) in the developing germarium, expressed TJ (white) but little or no detectable Fas3 (red). Cells posterior to the developing germaria expressed Fas3 and were previously all termed basal cells and expected to intercalate to form the basal stalk. However, a subset of Fas3-positive cells immediately posterior to the germarium also expressed TJ. Those cells formed an EGC of increasing cell numbers, indicated by green brackets. (C', C") At 36 hr APF, some somatic cells contacting the posterior of the most advanced cyst expressed Fas3 strongly (white dots) and some did not (blue dots). Fas3-positive cells were counted as EGC cells and Fas3-negative cells as ICs. (D) At 48 hr APF, the most posterior germline cyst is about to leave the germarium in most samples. Here, the cyst (green) appears to have just started to leave, invading Fas3-positive cells in EGC territory. The Fas3-positive cells surrounding the cyst are indicated by a yellow bracket, while Fas3-positive and TJ-positive cells posterior to the cyst are indicated by green brackets. Average IC (red brackets) and EGC (green and yellow brackets) cell numbers are written underneath the brackets and were derived from counting (A) 5, (B) 5, (C) 10, and (D) 5 samples. (E-G) Three developing ovarioles from the same 56 hr APF ovary, representing progressively later stages of development from top to bottom, show the most posterior cyst leaving the germarium and surrounded by Fas3-positive, TJ-positive cells. (E, F) Fas3-positive, TJ-positive cells posterior to the germline (green bracket), and around the most posterior cyst (yellow bracket) were counted for these single samples and then (G) counted as on the budded cyst (magenta bracket) or anterior to the budded cyst and forming a secondary EGC posterior to the germarium (blue bracket).
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Reilein, A., Kogan, H. V., Misner, R., Park, K. S., & Kalderon, D. (2021). Adult stem cells and niche cells segregate gradually from common precursors that build the adult Drosophila ovary during pupal development. elife, 10, e69749.
Mouse Anti-FAS3 Monoclonal Antibody (7G10) (V2LY-0925-LY122) in IF
Promotion of FC production by the JAK-STAT pathway overrides the opposing influence of increased Wnt pathway activity and synergizes with loss of Wnt pathway activity to cause dramatic loss of highly proliferative FSCs.
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Melamed, D., & Kalderon, D. (2020). Opposing JAK-STAT and Wnt signaling gradients define a stem cell domain by regulating differentiation at two borders. Elife, 9, e61204.
Mouse Anti-FAS3 Monoclonal Antibody (7G10) (V2LY-0925-LY122) in IHC
(A) A single cell in the posterior lobe labeled with Raeppli-mTFP1 (green) spans the height of the tissue labeled with lateral membrane marker Fasciclin III (Fas3; magenta). Apical side of posterior lobe identified with dotted line. Sample is 44 hr after pupal formation (APF), but was heat shocked for 1 hr at 24 hr APF causing it to develop faster and more closely resembles a 48 hr APF sample. Scale bar, 10 μm. n = 10 cells (B-E) Maximum projections of early (36 hr APF) and late (52 hr APF) genital samples labeled with Fas3 (lateral membranes, green) and E-Cadherin (Ecad; apical membranes; magenta). Location of respective cross sections indicated in yellow for lateral plate (B1-E1) and blue for posterior lobe (D. melanogaster) (B2-C2) or equivalent location in non-lobed species (D. biarmipes) (D2-E2). Scale bar, 20 μm. Relevant structures are labeled: posterior lobe (PL), lateral plate (LP), clasper (C).
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Smith, S. J., Davidson, L. A., & Rebeiz, M. (2020). Evolutionary expansion of apical extracellular matrix is required for the elongation of cells in a novel structure. Elife, 9, e55965.
Mouse Anti-FAS3 Monoclonal Antibody (7G10) (V2LY-0925-LY122) in IF
Dpp-GFP and TkvCA overexpression in mesoderm rescues Dpp signaling in Pngl-/- embryo.
(A-C) Projection views of stage 14 embryos of the indicated genotypes stained with anti-pMad (red) and anti-GFP (green) antibodies. (D-F') Close-ups of embryonic PS3 and PS7 of indicated genotypes. Notably, in the Pngl-/- embryo, Mef2>dpp GFP overexpression rescues pMad staining in PS3 and PS7 (E, compared to F). (G-H') Close-ups of embryonic PS3 and PS7 of indicated genotypes. pMad is marked in red and Fas3 in green. Arrows mark pMad staining outside of PS7. (I) Eclosion tests of the indicated genotypes. The red dashed line marks the expected Mendelian ratio. ...View More
(A-C) Projection views of stage 14 embryos of the indicated genotypes stained with anti-pMad (red) and anti-GFP (green) antibodies. (D-F') Close-ups of embryonic PS3 and PS7 of indicated genotypes. Notably, in the Pngl-/- embryo, Mef2>dpp GFP overexpression rescues pMad staining in PS3 and PS7 (E, compared to F). (G-H') Close-ups of embryonic PS3 and PS7 of indicated genotypes. pMad is marked in red and Fas3 in green. Arrows mark pMad staining outside of PS7. (I) Eclosion tests of the indicated genotypes. The red dashed line marks the expected Mendelian ratio. ...View More
Galeone, A., Han, S. Y., Huang, C., Hosomi, A., Suzuki, T., & Jafar-Nejad, H. (2017). Tissue-specific regulation of BMP signaling by Drosophila N-glycanase 1. elife, 6, e27612.
Datasheet
Target
Q & As
Review & reward
Protocols
Associated Products
Basic Information
Host Animal
Mouse
Clone
7G10
Application
IF, IHC, IP
Immunogen
Membrane preparation from nerve cord and cultures of primary neurons (10-13 hr development).
Host Species
Mouse
Specificity
Fruit Fly
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
| Application | Note |
| IHC | 2-5 µg/ml |
| IF(ICC) | 2-5 µg/ml |
Formulations & Storage [For reference only, actual COA shall prevail!]
Format
Supernatant
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
More Infomation
Target
Full Name
Fas3
Entrez Gene ID
UniProt ID
Research Area
Mediates cell adhesion in a Ca2+-independent manner. It plays a role in axon outgrowth, guidance and fasciculation of the developing nervous system.
Biological Process
Axonal fasciculation Source: FlyBase
Axon guidance Source: FlyBase
Cell-cell adhesion Source: GO_Central
Homophilic cell adhesion via plasma membrane adhesion molecules Source: FlyBase
Synaptic target recognition Source: FlyBase
Axon guidance Source: FlyBase
Cell-cell adhesion Source: GO_Central
Homophilic cell adhesion via plasma membrane adhesion molecules Source: FlyBase
Synaptic target recognition Source: FlyBase
Cellular Location
Membrane
Topology
Extracellular: 21-346
Helical: 347-370
Cytoplasmic: 371-508
Helical: 347-370
Cytoplasmic: 371-508
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Please try the standard protocols which include: protocols, troubleshooting and guide.
Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols
For research use only. Not intended for any clinical use.
Custom Antibody Labeling
We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).
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