Mouse Anti-FBXO3 Recombinant Antibody (CBXF-1729) (V2LY-0925-LY447)

Name: Mouse Anti-FBXO3 Recombinant Antibody (CBXF-1729)
SKU: V2LY-0925-LY447
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-FBXO3 Recombinant Antibody (CBXF-1729) (V2LY-0925-LY447) in WB

Western blotting (WB) analysis of FBXO3 in the peri-infarcted cortex of SD rats at MCAO 1 h/R 6, 12, 24, 48, 72 h, and sham treatment. ...View More

Gao, Y., Xiao, X., Luo, J., Wang, J., Peng, Q., Zhao, J., ... & Zhao, Y. (2022). E3 ubiquitin ligase FBXO3 drives neuroinflammation to aggravate cerebral ischemia/reperfusion injury. International Journal of Molecular Sciences, 23(21), 13648.

Mouse Anti-FBXO3 Recombinant Antibody (CBXF-1729) (V2LY-0925-LY447) in IF

Representative images (400×, scale bar = 100 μm) of FBXO3 (green)/NeuN (neuronal biomarker, red)/DAPI (blue), FBXO3/Iba-1 (microglial biomarker, red)/DAPI, and FBXO3/GFAP (astrocytic biomarker, red)/ DAPI immunostaining in the ischemic penumbra at sham and MCAO 1 h/R 24 h treatment, respectively. ...View More

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBXF-1729

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 91-390 mapping within an internal region of human FBXO3.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG2a, κ

Clonality

Monoclonal

Application Notes

Application	Note
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500
ELISA	1:100-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Gelatin & PBS

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

F-box protein 3

Entrez Gene ID

Human	26273
Mouse	57443
Rat	690634

UniProt ID

Human	Q9UK99
Mouse	Q9DC63
Rat	D4ABP9

Research Area

Substrate recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation.

Biological Process

Proteolysis Source: ProtInc

Cellular Location

Nucleus. Colocalizes with PML at the peripheries of nuclear bodies.

Wang, Q., Huang, P. Y., Wu, J. G., Zhang, T. Q., Li, L. F., Huang, L. D., ... & He, J. (2022). miR‐219a‐5p inhibits the pyroptosis in knee osteoarthritis by inactivating the NLRP3 signaling via targeting FBXO3. Environmental Toxicology, 37(11), 2673-2682.

Gao, Y., Xiao, X., Luo, J., Wang, J., Peng, Q., Zhao, J., ... & Zhao, Y. (2022). E3 Ubiquitin Ligase FBXO3 Drives Neuroinflammation to Aggravate Cerebral Ischemia/Reperfusion Injury. International Journal of Molecular Sciences, 23(21), 13648.

Niu, M., He, Y., Xu, J., Ding, L., He, T., Yi, Y., ... & Xiao, Z. X. J. (2021). Noncanonical TGF-β signaling leads to FBXO3-mediated degradation of ΔNp63α promoting breast cancer metastasis and poor clinical prognosis. PLoS biology, 19(2), e3001113.

Hsieh, M. C., Ho, Y. C., Lai, C. Y., Wang, H. H., Yang, P. S., Cheng, J. K., ... & Peng, H. Y. (2021). Blocking the Spinal Fbxo3/CARM1/K+ Channel Epigenetic Silencing Pathway as a Strategy for Neuropathic Pain Relief. Neurotherapeutics, 18(2), 1295-1315.

Li, J., & Ma, L. (2020). MiR-142-3p attenuates oxygen glucose deprivation/reoxygenation-induced injury by targeting FBXO3 in human neuroblastoma SH-SY5Y cells. World Neurosurgery, 136, e149-e157.

Li, Z., Fan, S., Wang, J., Chen, X., Liao, Q., Liu, X., ... & Xiao, W. (2020). Zebrafish F-box protein fbxo3 negatively regulates antiviral response through promoting K27-linked polyubiquitination of the transcription factors irf3 and irf7. The Journal of Immunology, 205(7), 1897-1908.

Watanabe, Y., Nakagawa, T., Akiyama, T., Nakagawa, M., Suzuki, N., Warita, H., ... & Nakayama, K. (2020). An amyotrophic lateral sclerosis-associated mutant of C21ORF2 is stabilized by NEK1-mediated hyperphosphorylation and the inability to bind FBXO3. Iscience, 23(9), 101491.

Hung, K. Y., Liao, W. I., Pao, H. P., Wu, S. Y., Huang, K. L., & Chu, S. J. (2019). Targeting F-box protein Fbxo3 attenuates lung injury induced by ischemia-reperfusion in rats. Frontiers in Pharmacology, 10, 583.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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