Mouse Anti-FGFR4 Recombinant Antibody (4FR6D3) (CBMAB-C0932-YY)

Name: Mouse Anti-FGFR4 Recombinant Antibody (4FR6D3)
SKU: CBMAB-C0932-YY
Rating: 5 (1 reviews)

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Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

4FR6D3

Application

FC, IF

Immunogen

NIH-3T3 cells transfected with full-length human FGFR4

Specificity

Human

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Concentration

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Fibroblast Growth Factor Receptor 4

Entrez Gene ID

2264

UniProt ID

P22455

Alternative Names

CD3e Molecule; CD3e Antigen, Epsilon Polypeptide (TiT3 Complex); T-Cell Surface Antigen T3/Leu-4 Epsilon Chain; CD3e Molecule, Epsilon (CD3-TCR Complex); T3E; T-Cell Antigen Receptor Complex, Epsilon Subunit Of T3;

Function

Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.

Biological Process

Cell migration Source: UniProtKB
Cholesterol homeostasis Source: UniProtKB
Fibroblast growth factor receptor signaling pathway Source: UniProtKB
Glucose homeostasis Source: UniProtKB
Peptidyl-tyrosine phosphorylation Source: UniProtKB
Phosphate ion homeostasis Source: UniProtKB
Positive regulation of catalytic activity Source: UniProtKB
Positive regulation of cell population proliferation Source: UniProtKB
Positive regulation of DNA biosynthetic process Source: UniProtKB
Positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
Positive regulation of gene expression Source: UniProtKB
Positive regulation of kinase activity Source: GO_Central
Positive regulation of proteolysis Source: UniProtKB
Protein autophosphorylation Source: UniProtKB
Regulation of bile acid biosynthetic process Source: UniProtKB
Regulation of extracellular matrix disassembly Source: UniProtKB
Regulation of lipid metabolic process Source: UniProtKB
Transmembrane receptor protein tyrosine kinase signaling pathway Source: GO_Central

Cellular Location

Endoplasmic reticulum; Endosome; Cell membrane. Internalized from the cell membrane to recycling endosomes, and from there back to the cell membrane.
Isoform 2: Secreted
Isoform 3: Cytoplasm

Involvement in disease

FGFR4 variants may be involved in the pathogenesis of various cancers. Variant Arg-388 predisposes cancer patients to accelerated disease progression and may be associated with poor prognosis. It has been found in prostate cancer as well as cancers of the breast, colon, head and neck, larynx, lung, skin.

Topology

Extracellular: 22-369
Helical: 370-390
Cytoplasmic: 391-802

PTM

N-glycosylated. Full maturation of the glycan chains in the Golgi is essential for high affinity interaction with FGF19.
Ubiquitinated. Subject to proteasomal degradation when not fully glycosylated.
Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer.

Tao, Z., Cui, Y., Xu, X., & Han, T. (2022). FGFR redundancy limits the efficacy of FGFR4-selective inhibitors in hepatocellular carcinoma. Proceedings of the National Academy of Sciences, 119(40), e2208844119.

Kanzaki, H., Chiba, T., Ao, J., Koroki, K., Kanayama, K., Maruta, S., ... & Kato, N. (2021). The impact of FGF19/FGFR4 signaling inhibition in antitumor activity of multi-kinase inhibitors in hepatocellular carcinoma. Scientific reports, 11(1), 1-12.

Chen, T., Liu, H., Liu, Z., Li, K., Qin, R., Wang, Y., ... & Xu, Y. (2021). FGF19 and FGFR4 promotes the progression of gallbladder carcinoma in an autocrine pathway dependent on GPBAR1-cAMP-EGR1 axis. Oncogene, 40(30), 4941-4953.

Garcia-Recio, S., Thennavan, A., East, M. P., Parker, J. S., Cejalvo, J. M., Garay, J. P., ... & Perou, C. M. (2020). FGFR4 regulates tumor subtype differentiation in luminal breast cancer and metastatic disease. The Journal of clinical investigation, 130(9), 4871-4887.

Levine, K. M., Ding, K., Chen, L., & Oesterreich, S. (2020). FGFR4: A promising therapeutic target for breast cancer and other solid tumors. Pharmacology & therapeutics, 214, 107590.

Liu, Y., Cao, M., Cai, Y., Li, X., Zhao, C., & Cui, R. (2020). Dissecting the role of the FGF19-FGFR4 signaling pathway in cancer development and progression. Frontiers in cell and developmental biology, 8, 95.

Rezende Miranda, R., Fu, Y., Chen, X., Perino, J., Cao, P., Carpten, J., ... & Zhang, C. (2020). Development of a potent and specific FGFR4 inhibitor for the treatment of hepatocellular carcinoma. Journal of Medicinal Chemistry, 63(20), 11484-11497.

Raja, A., Park, I., Haq, F., & Ahn, S. M. (2019). FGF19–FGFR4 signaling in hepatocellular carcinoma. Cells, 8(6), 536.

Hatlen, M. A., Schmidt-Kittler, O., Sherwin, C. A., Rozsahegyi, E., Rubin, N., Sheets, M. P., ... & Hoeflich, K. P. (2019). Acquired On-Target Clinical Resistance Validates FGFR4 as a Driver of Hepatocellular CarcinomaAcquired Resistance to FGFR4-Targeted Therapy in HCC. Cancer Discovery, 9(12), 1686-1695.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

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For research use only. Not intended for any clinical use.

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