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Mouse Anti-G3BP1 Antibody (2H8) (CBMAB-0401-YC)

Provided herein are mouse monoclonal antibodies against Human G3BP1. The antibody clone 2H8 can be used for immunoassay techniques, such as ELISA and IP.
See all G3BP1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2H8
Antibody Isotype
IgG2a
Application
ELISA, IP

Basic Information

Immunogen
Recombinant protein
Specificity
Human
Antibody Isotype
IgG2a
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
G3BP1
Introduction
G3BP1 (G3BP stress granule assembly factor 1) is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. G3BP1 is one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme
Entrez Gene ID
10146
UniProt ID
Q13283
Alternative Names
G3BP Stress Granule Assembly Factor 1; GTPase Activating Protein (SH3 Domain) Binding Protein 1; Ras-GTPase-Activating Protein SH3-Domain-Binding Protein; GAP SH3 Domain-Binding Protein 1; ATP-Dependent DNA Helicase VIII; DNA Helicase VIII; G3BP-1; G3BP; RasGAP-Associated Endoribonuclease G3BP; GAP Binding Protein; EC 3.6.4.12; EC 3.6.4.13; HDH-VIII; HDH VIII; EC 3.6.1
Function
ATP- and magnesium-dependent helicase that plays an essential role in innate immunity (PubMed:30510222).

Participates in the DNA-triggered cGAS/STING pathway by promoting the DNA binding and activation of CGAS. Enhances also DDX58-induced type I interferon production probably by helping DDX58 at sensing pathogenic RNA (PubMed:30804210).

In addition, plays an essential role in stress granule formation (PubMed:12642610, PubMed:20180778, PubMed:23279204).

Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends (PubMed:9889278).

Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency (PubMed:9889278).

Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA (PubMed:9889278).

Phosphorylation-dependent sequence-specific endoribonuclease in vitro (PubMed:11604510).

Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR (PubMed:11604510).
Biological Process
Defense response to virus Source: UniProtKB
Innate immune response Source: UniProtKB-KW
Negative regulation of canonical Wnt signaling pathway Source: Ensembl
Positive regulation of stress granule assembly Source: UniProtKB
Positive regulation of type I interferon production Source: UniProtKB
Ras protein signal transduction Source: ProtInc
Stress granule assembly Source: UniProtKB
Cellular Location
Cytosol; Nucleus; Perikaryon; Stress granule. Cytoplasmic in proliferating cells (PubMed:11604510). Cytosolic and partially nuclear in resting cells (PubMed:11604510). Recruited to stress granules in response to arsenite treatment (PubMed:12642610, PubMed:20180778). The unphosphorylated form is recruited to stress granules (PubMed:12642610). HRAS signaling contributes to this process by regulating G3BP dephosphorylation (PubMed:12642610).
PTM
(Microbial infection) Cleaved by human enterovirus 71; this cleavage induces the disassembly of cytoplasmic stress granules (PubMed:30006004). Cleaved by Foot-and-mouth disease virus; this cleavage suppresses the formation of cytoplasmic stress granules (PubMed:30404792).
Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity (By similarity). RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.
Arg-435 is dimethylated, probably to asymmetric dimethylarginine.
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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