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Mouse Anti-GABBR1 Monoclonal Antibody (S93A-49) (CBMAB-1307-YC)

Provided herein is a mouse monoclonal antibody against Human GABABR1. The antibody, clone S93A-49, can be used for immunoassay techniques, such as IHC and WB.
See all GABBR1 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse, Rat
Clone
S93A-49
Antibody Isotype
IgG1
Application
IHC, WB

Basic Information

Immunogen
Fusion protein corresponding to aa 873-977 (cytoplasmic C-terminus) of rat GABA(B)R1
Specificity
Human, Mouse, Rat
Antibody Isotype
IgG1
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
>95%, as determined by SDS-PAGE analysis
Storage
Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
gamma-aminobutyric acid type B receptor subunit 1
Introduction
GABABR1 is a receptor for gamma-aminobutyric acid (GABA), which is the main inhibitory neurotransmitter in the mammalian central nervous system. GABABR1 functions as a heterodimer with GABA(B) receptor 2. Defects in GABABR1 may underlie brain disorders such as schizophrenia and epilepsy.
Entrez Gene ID
Human2550
Mouse54393
Rat81657
UniProt ID
HumanQ9UBS5
MouseQ9WV18
RatQ9Z0U4
Alternative Names
GB1; GPRC3A; GABABR1; GABBR1-3
Function
Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed:9872316, PubMed:9872744, PubMed:15617512, PubMed:18165688, PubMed:22660477, PubMed:24305054).

Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (PubMed:18165688).

Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed:10906333, PubMed:10773016, PubMed:10075644, PubMed:9872744, PubMed:24305054).

Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis (PubMed:10075644).

Calcium is required for high affinity binding to GABA (By similarity).

Plays a critical role in the fine-tuning of inhibitory synaptic transmission (PubMed:9844003).

Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed:9844003, PubMed:9872316, PubMed:10075644, PubMed:9872744, PubMed:22660477).

Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (Probable). Activated by (-)-baclofen, cgp27492 and blocked by phaclofen (PubMed:9844003, PubMed:9872316, PubMed:24305054).

Isoform 1E may regulate the formation of functional GABBR1/GABBR2 heterodimers by competing for GABBR2 binding. This could explain the observation that certain small molecule ligands exhibit differential affinity for central versus peripheral sites.
Biological Process
Adenylate cyclase-inhibiting G protein-coupled receptor signaling pathway Source: UniProtKB
Gamma-aminobutyric acid signaling pathway Source: UniProtKB
Neuron-glial cell signaling Source: ARUK-UCL
Cellular Location
Cell membrane; Postsynaptic cell membrane; Dendrite. Colocalizes with ATF4 in hippocampal neuron dendritic membranes (By similarity). Coexpression of GABBR1 and GABBR2 is required for GABBR1 maturation and transport to the plasma membrane (PubMed:15617512).
Isoform 1E: Secreted
Topology
Extracellular: 15-591
Helical: 592-612
Cytoplasmic: 613-631
Helical: 632-652
Extracellular: 653-667
Helical: 668-688
Cytoplasmic: 689-710
Helical: 711-731
Extracellular: 732-768
Helical: 769-789
Cytoplasmic: 790-804
Helical: 805-825
Extracellular: 826-833
Helical: 834-854
Cytoplasmic: 855-961
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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