Sign in or Register   Sign in or Register
  |  

Rat Anti-GATA1 Recombinant Antibody (N1) (CBMAB-G2186-LY)

This product is antibody recognizes GATA1. The antibody N1 immunoassay techniques such as: WB.
See all GATA1 antibodies

Summary

Host Animal
Rat
Specificity
Mouse, Rat, Human
Clone
N1
Antibody Isotype
IgG
Application
WB

Basic Information

Specificity
Mouse, Rat, Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
GATA Binding Protein 1
Introduction
This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. [provided by RefSeq, Jul 2008]
Entrez Gene ID
Human2623
Mouse14460
Rat25172
UniProt ID
HumanP15976
MouseP17679
RatP43429
Function
Transcriptional activator or repressor which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed:24245781).
Biological Process
Basophil differentiation Source: BHF-UCL
Cell-cell signaling Source: Ensembl
Cell fate commitment Source: GO_Central
Cellular response to thyroid hormone stimulus Source: UniProtKB
Dendritic cell differentiation Source: Ensembl
Embryonic hemopoiesis Source: Ensembl
Eosinophil differentiation Source: BHF-UCL
Eosinophil fate commitment Source: BHF-UCL
Erythrocyte development Source: BHF-UCL
Erythrocyte differentiation Source: BHF-UCL
Homeostasis of number of cells within a tissue Source: Ensembl
In utero embryonic development Source: Ensembl
Male gonad development Source: BHF-UCL
Megakaryocyte differentiation Source: BHF-UCL
Negative regulation of apoptotic process Source: UniProtKB
Negative regulation of bone mineralization Source: Ensembl
Negative regulation of cell population proliferation Source: Ensembl
Negative regulation of extrinsic apoptotic signaling pathway in absence of ligand Source: BHF-UCL
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Negative regulation of transcription regulatory region DNA binding Source: BHF-UCL
Platelet aggregation Source: BHF-UCL
Platelet formation Source: BHF-UCL
Positive regulation of erythrocyte differentiation Source: UniProtKB
Positive regulation of osteoblast proliferation Source: Ensembl
Positive regulation of peptidyl-tyrosine phosphorylation Source: BHF-UCL
Positive regulation of transcription, DNA-templated Source: UniProtKB
Positive regulation of transcription by RNA polymerase II Source: BHF-UCL
Regulation of definitive erythrocyte differentiation Source: BHF-UCL
Regulation of glycoprotein biosynthetic process Source: BHF-UCL
Regulation of primitive erythrocyte differentiation Source: Ensembl
Regulation of transcription by RNA polymerase II Source: GO_Central
Cellular Location
Nucleus
Involvement in disease
X-linked dyserythropoietic anemia and thrombocytopenia (XDAT):
Disorder characterized by erythrocytes with abnormal size and shape, and paucity of platelets in peripheral blood. The bone marrow contains abundant and abnormally small megakaryocytes.
Thrombocytopenia with beta-thalassemia, X-linked (XLTT):
An unusual form of thrombocytopenia associated with beta-thalassemia. Patients have splenomegaly and petechiae, moderate thrombocytopenia, prolonged bleeding time due to platelet dysfunction, reticulocytosis and unbalanced (hemo)globin chain synthesis resembling that of beta-thalassemia minor.
Anemia without thrombocytopenia, X-linked (XLAWT):
A form of anemia characterized by abnormal morphology of erythrocytes and granulocytes in peripheral blood, bone marrow dysplasia with hypocellularity of erythroid and granulocytic lineages, and normal or increased number of megakaryocytes. Neutropenia of a variable degree is present in affected individuals.
PTM
Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137 (By similarity).
Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation with SUMO1 has no effect on transcriptional activity (By similarity).
Acetylated at 2 conserved lysine-rich motifs by CREBBP in vitro. Acetylation does not affect DNA-binding in vitro but is essential to induce erythroid differentiation and for binding chromatin in vivo (By similarity). Acetylated on Lys-233, Lys-245 Lys-246 by EP300.
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Rat Anti-GATA1 Recombinant Antibody (N1)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Go to
Compare