Mouse Anti-GEMIN2 Recombinant Antibody (CBLG1-2699) (V2LY-1225-LY1048)

Name: Mouse Anti-GEMIN2 Recombinant Antibody (CBLG1-2699)
SKU: V2LY-1225-LY1048
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-GEMIN2 Recombinant Antibody (CBLG1-2699) (V2LY-1225-LY1048) in WB

Knockdown of Gemin3 and Gemin2 was assessed by western blotting analysis. αB-crystallin and γ-tubulin were used as loading controls. ...View More

den Engelsman, J., van de Schootbrugge, C., Yong, J., Pruijn, G. J., & Boelens, W. C. (2013). Pseudophosphorylated αB-crystallin is a nuclear chaperone imported into the nucleus with help of the SMN complex. PloS one, 8(9), e73489.

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBLG1-2699

Application

WB, IP, IF, ELISA

Immunogen

Amino acids 2-101 mapping at the N-terminus of human Gemin2.

Host Species

Mouse

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG1, κ

Clonality

Monoclonal

Application Notes

Application	Note
ELISA	1:100-1:1,000
WB	1:100-1:1,000
IP	1-2 µg per 100-500 µg of total protein (1 ml of cell lysate)
IF(ICC)	1:50-1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, gelatin

Preservative

Sodium azide

Concentration

0.2 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

GEMIN2

Entrez Gene ID

Human	8487
Mouse	66603
Rat	84404

UniProt ID

Human	O14893
Mouse	Q9CQQ4
Rat	Q9QZP1

Function

The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs (PubMed:18984161, PubMed:9323129).

Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core) (PubMed:18984161).

In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG (5Sm) are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP (PubMed:18984161).

To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A (PubMed:18984161, PubMed:9323129).

Binding of snRNA inside 5Sm ultimately triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP (PubMed:31799625).

Within the SMN complex, GEMIN2 constrains the conformation of 5Sm, thereby promoting 5Sm binding to snRNA containing the snRNP code (a nonameric Sm site and a 3'-adjacent stem-loop), thus preventing progression of assembly until a cognate substrate is bound (PubMed:31799625, PubMed:21816274, PubMed:16314521).

Biological Process

mRNA processing Source: ProtInc
RNA splicing Source: UniProtKB
RNA splicing, via transesterification reactions Source: UniProtKB
Spliceosomal complex assembly Source: ProtInc
Spliceosomal snRNP assembly Source: UniProtKB

Cellular Location

Gem; Cytoplasm. Localized in subnuclear structures next to coiled bodies, called gems, which are highly enriched in spliceosomal snRNPs. Also found in the cytoplasm.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Rabbit Anti-GEMIN2 Recombinant Antibody (CBLG1-2699) (CAT#: CBMAB-G2794-LY)

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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