Rabbit Anti-GEN1 Recombinant Antibody (14D68) (V2LY-1225-LY1069)

Name: Rabbit Anti-GEN1 Recombinant Antibody (14D68)
SKU: V2LY-1225-LY1069
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

14D68

Application

Immunogen

Human GEN1.

Host Species

Rabbit

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

Application	Note
WB	1:500-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, BSA, glycerol

Preservative

Sodium azide

Concentration

1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

GEN1, Holliday Junction 5' Flap Endonuclease

Entrez Gene ID

348654

UniProt ID

Q17RS7

Function

Endonuclease which resolves Holliday junctions (HJs) by the introduction of symmetrically related cuts across the junction point, to produce nicked duplex products in which the nicks can be readily ligated. Four-way DNA intermediates, also known as Holliday junctions, are formed during homologous recombination and DNA repair, and their resolution is necessary for proper chromosome segregation (PubMed:19020614, PubMed:26682650).

Cleaves HJs by a nick and counter-nick mechanism involving dual coordinated incisions that lead to the formation of ligatable nicked duplex products. Cleavage of the first strand is rate limiting, while second strand cleavage is rapid. Largely monomeric, dimerizes on the HJ and the first nick occurs upon dimerization at the junction (PubMed:26578604).

Efficiently cleaves both single and double HJs contained within large recombination intermediates. Exhibits a weak sequence preference for incision between two G residues that reside in a T-rich region of DNA (PubMed:28049850).

Has also endonuclease activity on 5'-flap and replication fork (RF) DNA substrates (PubMed:26578604).

Biological Process

Double-strand break repair via homologous recombination Source: UniProtKB
Positive regulation of mitotic cell cycle spindle assembly checkpoint Source: UniProtKB
Regulation of centrosome duplication Source: UniProtKB
Replication fork processing Source: UniProtKB
Resolution of mitotic recombination intermediates Source: UniProtKB
Resolution of recombination intermediates Source: UniProtKB

Cellular Location

Nucleus

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Alternative Versions

Mouse Anti-GEN1 Recombinant Antibody (14D68) (CAT#: CBMAB-G2832-LY)

Related Products

Mouse Anti-GEN1 Recombinant Antibody (14D68) (CAT#: CBMAB-G2832-LY)

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Mouse Anti-GEN1 Recombinant Antibody (CBLG1-857) (CAT#: CBMAB-G2841-LY)

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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