Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-GFI1 Recombinant Antibody (CBT616) (V2LY-0625-LY3385)


See all GFI1 antibodies
Online Inquiry
Tested Data
Mouse Anti-GFI1 Recombinant Antibody (CBT616) (V2LY-0625-LY3385) in WB
Mouse Anti-GFI1 Recombinant Antibody (CBT616) (V2LY-0625-LY3385) in WB

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBT616
Antibody Isotype
IgG
Application
WB, ELISA

Basic Information

Immunogen
Purified recombinant fragment of human Gfi-1 expressed in E. Coli.
Host Species
Mouse
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal Antibody
Application Notes
ApplicationNote
ELISA1:10,000
WB1:500-1:2,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Supernatant
Buffer
BSA, Glycerol
Preservative
Sodium azide
Concentration
Batch dependent
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Entrez Gene ID
2672
UniProt ID
Q99684
Function
Transcription repressor essential for hematopoiesis. Functions in a cell-context and development-specific manner. Binds to 5'-TAAATCAC[AT]GCA-3' in the promoter region of a large number of genes. Component of several complexes, including the EHMT2-GFI1-HDAC1, AJUBA-GFI1-HDAC1 and RCOR-GFI-KDM1A-HDAC complexes, that suppress, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Regulates neutrophil differentiation, promotes proliferation of lymphoid cells, and is required for granulocyte development. Mediates, together with U2AF1L4, the alternative splicing of CD45 and controls T-cell receptor signaling. Regulates the endotoxin-mediated Toll-like receptor (TLR) inflammatory response by antagonizing RELA. Cooperates with CBFA2T2 to regulate ITGB1-dependent neurite growth. Controls cell-cycle progression by repressing CDKNIA/p21 transcription in response to TGFB1 via recruitment of GFI1 by ZBTB17 to the CDKNIA/p21 and CDKNIB promoters. Required for the maintenance of inner ear hair cells.
Biological Process
Cellular response to lipopolysaccharide Source: UniProtKB
Hemopoiesis Source: InterPro
Negative regulation of calcidiol 1-monooxygenase activity Source: BHF-UCL
Negative regulation of neuron projection development Source: UniProtKB
Negative regulation of NF-kappaB transcription factor activity Source: UniProtKB
Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: UniProtKB
Negative regulation of vitamin D biosynthetic process Source: BHF-UCL
Positive regulation of interleukin-6-mediated signaling pathway Source: UniProtKB
Regulation of histone H3-K4 methylation Source: InterPro
Regulation of toll-like receptor signaling pathway Source: UniProtKB
Regulation of transcription by RNA polymerase II Source: GO_Central
Regulation of transcription involved in G1/S transition of mitotic cell cycle Source: ProtInc
Viral process Source: ProtInc
Cellular Location
Nucleus. Colocalizes with PIAS3 and RUNX1T1 in nuclear dots.
Involvement in disease
Neutropenia, severe congenital 2, autosomal dominant (SCN2):
A disorder of hematopoiesis characterized by maturation arrest of granulopoiesis at the level of promyelocytes with peripheral blood absolute neutrophil counts below 0.5 x 10(9)/l and early onset of severe bacterial infections.
Dominant nonimmune chronic idiopathic neutropenia of adults (NI-CINA):
Relatively mild form of neutropenia diagnosed in adults, but predisposing to leukemia in a subset of patients.
PTM
Ubiquitinated. Ubiquitination and degradation by the proteasome is inhibited by the ubiquitin ligase, ARIH2.
More Infomation
Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-GFI1 Recombinant Antibody (CBT616)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry