Mouse Anti-GFP Recombinant Antibody (229) (CBMAB-G0387-LY)

Name: Mouse Anti-GFP Recombinant Antibody (229)
SKU: CBMAB-G0387-LY
Rating: 5 (1 reviews)

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Published Data

Mouse Anti-GFP Recombinant Antibody (229) (CBMAB-G0387-LY) in IF

pRab10 localizes on peri-centriolar membranes. HeLa cells stably expressing GFP-Rab10 were transfected with or without LRRK2-R1441G plasmid. After 24 hr, cells were fixed and stained with the indicated antibodies. (A) Localization of total GFP-Rab10 (green) without (left) or with (right) R1441G-LRRK2 transfection. (B) Quantitation of peri-nuclear clustering of GFP-Rab10. (C) Cells were stained with rabbit anti-pRab10 antibody (red) continued on next page and co-stained with either mouse anti-GFP (green), mouse anti-Centrin-3 (green) or mouse anti-transferrin receptor (TfR, green). (D) HEK293T cells were co-transfected with myc-LRRK2-R1441G and RILPL1-GFP (green). After 24 hr, cells were fixed and stained for pRab10 (red) and LRRK2 (blue). Dotted lines indicate cell boundaries. Dotted boxes indicate the enlarged region of interest. Scale bars, 10 mm. ...View More

Dhekne, H. S., Yanatori, I., Gomez, R. C., Tonelli, F., Diez, F., Schüle, B., Steger, M., Alessi, D. R., & Pfeffer, S. R. (2018). A pathway for Parkinson's Disease LRRK2 kinase to block primary cilia and Sonic hedgehog signaling in the brain. eLife, 7, e40202.

Mouse Anti-GFP Recombinant Antibody (229) (CBMAB-G0387-LY) in IF

No co-localization of markers for GABA or glutamate in CSD neurons.

A schema is provided indicating the region of interest-the antennal lobes- with a square. In A-C, the expression pattern of the RN2-Gal4 driver targeting CSD neurons are visualized using UAS-mCD8::GFP (in green). A-A´´, the cell bodies of CSD neurons (green) do not colocalize with GABA staining (in magenta) or, in the region of the ALs, B-B´´, with DvGlut (in magenta). C-C´´, the cholinergic neurons targeted by the ChAT-Gal4 driver (in green) co-express serotonin 5-HT (in magenta). A-C shows a representative 1-μm-thick slice of a confocal stack. The white arrow in A-A´´ indicates the position of CSD neurons in the adult brain, and the yellow arrows in C indicate co- expression of respective markers. Scale bars represent 25 μm. ...View More

He, J., Hommen, F., Lauer, N., Balmert, S., & Scholz, H. (2020). Serotonin transporter dependent modulation of food-seeking behavior. PloS one, 15(1), e0227554.

Mouse Anti-GFP Recombinant Antibody (229) (CBMAB-G0387-LY) in Immunoblot

Immunoblots show reduced expression of several ribosomal proteins in (PR)175-GFP-expressing neurons. Calnexin was used as loading control. ...View More

Hartmann, H., Hornburg, D., Czuppa, M., Bader, J., Michaelsen, M., Farny, D., Arzberger, T., Mann, M., Meissner, F., & Edbauer, D. (2018). Proteomics and C9orf72 neuropathology identify ribosomes as poly-GR/PR interactors driving toxicity. Life science alliance, 1(2), e201800070.

Mouse Anti-GFP Recombinant Antibody (229) (CBMAB-G0387-LY) in IP

Coimmunoprecipitation of the transfected Myc-Numb65, Myc-Numb66, Myc-Numblike with EGFP-α-Actin (ACTC1) and EGFP-β-Actin in AD293 cells. ...View More

Wang, B., Yang, M., & Li, S. (2022). Numb and Numblike regulate sarcomere assembly and maintenance. The Journal of clinical investigation, 132(3), e139420.

Mouse Anti-GFP Recombinant Antibody (229) (CBMAB-G0387-LY) in Immunoblot and IP

Immunoblotting results showing the expression of GFP-L10a fusion protein only for a qualitative assessment. The original membranes were cut prior to hybridization with antibodies during blotting. ...View More

Yazar, V., Kang, S. U., Ha, S., Dawson, V. L., & Dawson, T. M. (2021). Integrative genome-wide analysis of dopaminergic neuron-specific PARIS expression in Drosophila dissects recognition of multiple PPAR-γ associated gene regulation. Scientific reports, 11(1), 21500.

Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

229

Application

ELISA, IF, IHC, IP, WB

Immunogen

Fusion protein amino acids 1-238 (full-length) of jellyfish green fluorescent protein.

Specificity

Jellyfish

Antibody Isotype

IgG2a

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Application	Note
WB	1:1,000
IF(ICC)	1:500
IHC	1:500

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

Tris

Preservative

Sodium azide

Concentration

1 mg/ml

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Green fluorescent protein

Function

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.

Biological Process

Bioluminescence
Generation of precursor metabolites and energy

PTM

Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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