Mouse Anti-GNPAT Recombinant Antibody (2E5C10) (CBMAB-AP2569LY)

See all GNPAT antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

2E5C10

Antibody Isotype

IgG2b

Application

ELISA, WB

Basic Information

Immunogen

Fusion Protein of GNPAT

Specificity

Human

Antibody Isotype

IgG2b

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

Glyceronephosphate O-Acyltransferase

Introduction

This gene encodes an enzyme located in the peroxisomal membrane which is essential to the synthesis of ether phospholipids. Mutations in this gene are associated with rhizomelic chondrodysplasia punctata. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

Entrez Gene ID

8443

UniProt ID

O15228

Alternative Names

Glyceronephosphate O-Acyltransferase; Glycerone-Phosphate O-Acyltransferase; Acyl-CoA:Dihydroxyacetonephosphateacyltransferase; Dihydroxyacetone Phosphate Acyltransferase; EC 2.3.1.42; DHAP-AT;

Function

Dihydroxyacetonephosphate acyltransferase involved in plasmalogen biosynthesis.

Biological Process

Cerebellum morphogenesis Source: Ensembl
Ether lipid biosynthetic process Source: UniProtKB
Fatty acid metabolic process Source: GO_Central
Membrane organization Source: Ensembl
Paranodal junction assembly Source: Ensembl
Phosphatidic acid biosynthetic process Source: Reactome
Phospholipid biosynthetic process Source: GO_Central
Response to drug Source: Ensembl
Response to fatty acid Source: Ensembl
Response to nutrient Source: Ensembl
Response to starvation Source: Ensembl
Synapse assembly Source: Ensembl

Cellular Location

Peroxisome membrane. Exclusively localized to the lumenal side of the peroxisomal membrane.

Involvement in disease

Rhizomelic chondrodysplasia punctata 2 (RCDP2):
A form of rhizomelic chondrodysplasia punctata, a disease characterized by severely disturbed endochondral bone formation, rhizomelic shortening of femur and humerus, vertebral disorders, dwarfism, cataract, cutaneous lesions, facial dysmorphism, and severe mental retardation with spasticity.

References

Cordisco, A., Pelo, E., Di Tommaso, M., & Biagiotti, R. (2021). A new GNPAT variant of foetal rhizomelic chondrodysplasia punctata. Molecular Genetics & Genomic Medicine, 9(8), e1733.

Gu, L., Zhu, Y., Lin, X., Tan, X., Lu, B., & Li, Y. (2020). Stabilization of FASN by ACAT1-mediated GNPAT acetylation promotes lipid metabolism and hepatocarcinogenesis. Oncogene, 39(11), 2437-2449.

An, P., Wang, J., Wang, H., Jiang, L., Wang, J., Min, J., & Wang, F. (2020). Gnpat does not play an essential role in systemic iron homeostasis in murine model. Journal of Cellular and Molecular Medicine, 24(7), 4118-4126.

Secondes, E. S., Wallace, D. F., Rishi, G., McLaren, G. D., McLaren, C. E., Chen, W. P., ... & Subramaniam, V. N. (2020). Increased frequency of GNPAT p. D519G in compound HFE p. C282Y/p. H63D heterozygotes with elevated serum ferritin levels. Blood Cells, Molecules, and Diseases, 85, 102463.

Rishi, G., Secondes, E. S., Asplett, K., Wallace, D. F., Ostini, L., Berger, J., & Subramaniam, V. N. (2020). Dysregulated hepcidin response to dietary iron in male mice with reduced Gnpat expression. Bioscience Reports, 40(8).

Secondes, E. S., Wallace, D. F., Rishi, G., McLaren, G. D., McLaren, C. E., Chen, W. P., ... & Subramaniam, V. N. (2019). Increased Allele Frequency of GNPAT p. D519G in Compound HFE p. C282Y/p. H63D Heterozygotes with Elevated Serum Ferritin Levels. Blood, 134, 4807.

Q & As

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Review & reward

Have you used Mouse Anti-GNPAT Recombinant Antibody (2E5C10)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Rabbit Anti-GNPAT Recombinant Antibody (EG1344)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Documents

Datasheet
SDS
Request for COA

Request bulk or custom quote

Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email: