Rabbit Anti-GOLM1 Recombinant Antibody (
9C2) (V2LY-0725-LY727)

Name: Rabbit Anti-GOLM1 Recombinant Antibody (9C2)
SKU: V2LY-0725-LY727
Rating: 5 (1 reviews)

See all GOLM1 antibodies

Compare Online Inquiry

Tested Data

Rabbit Anti-GOLM1 Recombinant Antibody (9C2) (V2LY-0725-LY727) in WB

Western Blot
Positive WB detected in: SH-SY5Y whole cell lysate, HepG2 whole cell lysate
All lanes: GOLM1 antibody at 1.3μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 46, 45 KDa
Observed band size: 80 KDa

Rabbit Anti-GOLM1 Recombinant Antibody (9C2) (V2LY-0725-LY727) in FC

Overlay histogram showing Hela cells stained with V2LY-0725-LY727 (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4°C. Control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

9C2

Application

ELISA, WB, FC

Immunogen

A synthesized peptide derived from human GOLM1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Golgi Membrane Protein 1

Entrez Gene ID

51280

UniProt ID

Q8NBJ4

Function

Unknown. Cellular response protein to viral infection.

Biological Process

Nucleus organization Source: Ensembl
Regulation of lipid metabolic process Source: Ensembl

Cellular Location

Cis-Golgi network membrane. Early Golgi. Cycles via the cell surface and endosomes upon lumenal pH disruption.

Topology

Cytoplasmic: 1-12
Helical: 13-35
Lumenal: 36-401

PTM

Glycosylated.
Phosphorylation sites are present in the extracellular medium.

Shao, W. Q., Zhu, W. W., Luo, M. J., Fan, M. H., Li, Q., Wang, S. H., ... & Qin, L. X. (2022). Cholesterol suppresses GOLM1-dependent selective autophagy of RTKs in hepatocellular carcinoma. Cell Reports, 39(3), 110712.

Sun, Z., Mao, Y., Zhang, X., Lu, S., Wang, H., Zhang, C., ... & Li, K. (2021). Identification of ARHGEF38, NETO2, GOLM1, and SAPCD2 associated with prostate cancer progression by bioinformatic analysis and experimental validation. Frontiers in Cell and Developmental Biology, 9, 718638.

Chen, J., Lin, Z., Liu, L., Zhang, R., Geng, Y., Fan, M., ... & Qin, L. X. (2021). GOLM1 exacerbates CD8+ T cell suppression in hepatocellular carcinoma by promoting exosomal PD-L1 transport into tumor-associated macrophages. Signal transduction and targeted therapy, 6(1), 397.

Pu, Y., Song, Y., Zhang, M., Long, C., Li, J., Wang, Y., ... & Zhang, H. (2021). GOLM1 restricts colitis and colon tumorigenesis by ensuring Notch signaling equilibrium in intestinal homeostasis. Signal Transduction and Targeted Therapy, 6(1), 148.

Yan, J., Zhou, B., Li, H., Guo, L., & Ye, Q. (2020). Recent advances of GOLM1 in hepatocellular carcinoma. Hepatic oncology, 7(2), HEP22.

Yan, J., Zhou, B., Guo, L., Chen, Z., Zhang, B., Liu, S., ... & Ye, Q. (2020). GOLM1 upregulates expression of PD-L1 through EGFR/STAT3 pathway in hepatocellular carcinoma. American journal of cancer research, 10(11), 3705.

Lin, Y., Dong, H., Deng, W., Lin, W., Li, K., Xiong, X., ... & Zhang, H. (2019). Evaluation of Salivary Exosomal Chimeric GOLM1-NAA35 RNA as a Potential Biomarker in Esophageal CarcinomaExosomal Chimeric RNA in Cancer. Clinical Cancer Research, 25(10), 3035-3045.

Teerlink, C. C., Huff, C., Stevens, J., Yu, Y., Holmen, S. L., Silvis, M. R., ... & Cannon-Albright, L. A. (2018). A nonsynonymous variant in the GOLM1 gene in cutaneous malignant melanoma. JNCI: Journal of the National Cancer Institute, 110(12), 1380-1385.

Yan, G., Ru, Y., Wu, K., Yan, F., Wang, Q., Wang, J., ... & Zou, L. (2018). GOLM1 promotes prostate cancer progression through activating PI3K‐AKT‐mTOR signaling. The Prostate, 78(3), 166-177.

Yang, L., Luo, P., Song, Q., & Fei, X. (2018). DNMT1/miR-200a/GOLM1 signaling pathway regulates lung adenocarcinoma cells proliferation. Biomedicine & Pharmacotherapy, 99, 839-847.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Rabbit Anti-GOLM1 Recombinant Antibody (
9C2)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-GOLM1 Recombinant Antibody (2D6) (CAT#: CBMAB-G4311-LY)

Mouse Anti-GOLM1 Recombinant Antibody (CBLG1-174) (CAT#: CBMAB-G0624-LY)

Mouse Anti-GOLM1 Recombinant Antibody (CBLG1-2868) (CAT#: CBMAB-G0622-LY)

Mouse Anti-GOLM1 Recombinant Antibody (11F1A7) (CAT#: CBMAB-G4308-LY)

Mouse Anti-GOLM1 Recombinant Antibody (CBLG1-2870) (CAT#: CBMAB-G4313-LY)

Rabbit Anti-GOLM1 (AA 40-401) Recombinant Antibody (CBFYH-0418) (CAT#: CBMAB-H1288-FY)

Mouse Anti-GOLM1 Recombinant Antibody (CBLG1-1500) (CAT#: CBMAB-G4332-LY)

Mouse Anti-GOLM1 Recombinant Antibody (CBLG1-1524) (CAT#: CBMAB-G4356-LY)

Mouse Anti-GOLM1 Recombinant Antibody (CBLG1-2872) (CAT#: CBMAB-G4326-LY)

Mouse Anti-GOLM1 Recombinant Antibody (5B10) (CAT#: CBMAB-G4320-LY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry