Mouse Anti-GPR135 Recombinant Antibody (1H5) (CBMAB-G4631-LY)

This product is antibody recognizes GPR135 . The antibody 1H5 immunoassay techniques such as: ELISA, IHC, WB.
See all GPR135 antibodies

Datasheet

Summary

Host Animal

Mouse

Specificity

Human

Clone

1H5

Antibody Isotype

IgG

Application

ELISA, IHC, WB

Basic Information

Immunogen

GPR135 (NP_072093, aa391-493) partial recombinant protein with GST tag. MW of the GST tag alone is 26kD

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

G protein-coupled receptor 135

Entrez Gene ID

64582

UniProt ID

Q8IZ08

Alternative Names

HUMNPIIY20

Function

Orphan receptor. Has spontaneous activity for beta-arrestin recruitment (PubMed:28827538).

Shows a reciprocal regulatory interaction with the melatonin receptor MTNR1B most likely through receptor heteromerization (PubMed:28827538).

Biological Process

G protein-coupled receptor signaling pathway Source: GO_Central

Cellular Location

Endosome membrane; Cell membrane. Colocalizes with ARRB2/beta-arrestin-2 in the endosome.

Topology

Extracellular: 1-12
Helical: 13-33
Cytoplasmic: 34-37
Helical: 38-58
Extracellular: 59-81
Helical: 82-102
Cytoplasmic: 103-125
Helical: 126-146
Extracellular: 147-164
Helical: 165-185
Cytoplasmic: 186-226
Helical: 227-247
Extracellular: 248-262
Helical: 263-283
Cytoplasmic: 284-494

References

Gutiérrez-Ruiz, J. R., Villafaña, S., Ruiz-Hernández, A., Viruette-Pontigo, D., Menchaca-Cervantes, C., Aguayo-Cerón, K. A., ... & Romero-Nava, R. (2022). Expression profiles of GPR21, GPR39, GPR135, and GPR153 orphan receptors in different cancers. Nucleosides, Nucleotides & Nucleic Acids, 41(2), 123-136.

Watkins, L. R., & Orlandi, C. (2020). Orphan G protein coupled receptors in affective disorders. Genes, 11(6), 694.

Oishi, A., Cecon, E., & Jockers, R. (2018). Melatonin receptor signaling: impact of receptor oligomerization on receptor function. International Review of Cell and Molecular Biology, 338, 59-77.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Associated Products

Related Products

Rabbit Anti-GPR135 Recombinant Antibody (EG1374)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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