Rabbit Anti-GZMK Recombinant Antibody (EG1427) (CBMAB-EN1706-LY)

The product is antibody recognizes GRAK. The antibody EG1427 immunoassay techniques such as: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000.
See all GZMK antibodies

Datasheet

Summary

Host Animal

Rabbit

Specificity

Human, Mouse, Rat

Clone

EG1427

Antibody Isotype

IgG

Application

WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:20000

Basic Information

Immunogen

The antibody was produced against synthesized peptide derived from internal of human GRAK.

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name

granzyme K

Introduction

This gene product is a member of a group of related serine proteases from the cytoplasmic granules of cytotoxic lymphocytes. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the remarkable ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. The protein described here lacks consensus sequences for N-glycosylation present in other granzymes.

Entrez Gene ID

Human	3003
Mouse	14945
Rat	29165

UniProt ID

Human	P49863
Mouse	O35205
Rat	P49864

Alternative Names

TRYP2

Cellular Location

Secreted; Cytoplasmic granule

References

Turner, C. T., Zeglinski, M. R., Boivin, W., Zhao, H., Pawluk, M. A., Richardson, K. C., ... & Granville, D. J. (2022). Granzyme K contributes to endothelial microvascular damage and leakage during skin inflammation. British Journal of Dermatology, ljac017.

Kaiserman, D., Zhao, P., Rowe, C. L., Leong, A., Barlow, N., Joeckel, L. T., ... & Bird, P. I. (2022). Granzyme K initiates IL-6 and IL-8 release from epithelial cells by activating protease-activated receptor 2. PloS one, 17(7), e0270584.

Jonsson, A. H., Zhang, F., Dunlap, G., Gomez-Rivas, E., Watts, G. F., Faust, H. J., ... & Brenner, M. B. (2022). Granzyme K+ CD8 T cells form a core population in inflamed human tissue. Science translational medicine, 14(649), eabo0686.

Uranga-Murillo, I., Tapia, E., Garzón-Tituaña, M., Ramirez-Labrada, A., Santiago, L., Pesini, C., ... & Arias, M. (2021). Biological relevance of Granzymes A and K during E. coli sepsis. Theranostics, 11(20), 9873.

Tauh, K. (2020). Granzyme K: a novel contributor in cardiac allograft vasculopathy (Doctoral dissertation, University of British Columbia).

Turner, C. T., Zeglinski, M. R., Richardson, K. C., Zhao, H., Shen, Y., Papp, A., ... & Granville, D. J. (2019). Granzyme K expressed by classically activated macrophages contributes to inflammation and impaired remodeling. Journal of Investigative Dermatology, 139(4), 930-939.

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Protocols

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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