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Mouse Anti-HAND2 (AA 135-216) Recombinant Antibody (CBFYH-0677) (CBMAB-H1566-FY)

This product is mouse antibody that recognizes HAND2. The antibody CBFYH-0677 can be used for immunoassay techniques such as: ELISA, IF, WB.
See all HAND2 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYH-0677
Antibody Isotype
IgG2b, κ
Application
ELISA, IF, WB

Basic Information

Immunogen
Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 135-216

Target

Full Name
HAND2
Introduction
The protein encoded by this gene belongs to the basic helix-loop-helix family of transcription factors. This gene product is one of two closely related family members, the HAND proteins, which are asymmetrically expressed in the developing ventricular chambers and play an essential role in cardiac morphogenesis. Working in a complementary fashion, they function in the formation of the right ventricle and aortic arch arteries, implicating them as mediators of congenital heart disease. In addition, this transcription factor plays an important role in limb and branchial arch development.
Entrez Gene ID
UniProt ID
Alternative Names
Heart And Neural Crest Derivatives Expressed 2; Deciduum, Heart, Autonomic Nervous System And Neural Crest Derivatives-Expressed Protein 2; Class A Basic Helix-Loop-Helix Protein 26; BHLHa26; DHand; Heart- And Neural Crest Derivatives-Expressed Protein 2
Function
Essential for cardiac morphogenesis, particularly for the formation of the right ventricle and of the aortic arch arteries. Required for vascular development and regulation of angiogenesis, possibly through a VEGF signaling pathway. Plays also an important role in limb development, particularly in the establishment of anterior-posterior polarization, acting as an upstream regulator of sonic hedgehog (SHH) induction in the limb bud. Is involved in the development of branchial arches, which give rise to unique structures in the head and neck. Binds DNA on E-box consensus sequence 5'-CANNTG-3' (By similarity).
Biological Process
Adult heart development Source: BHF-UCL
Angiogenesis Source: UniProtKB-KW
Apoptotic process involved in heart morphogenesis Source: Ensembl
Cardiac neural crest cell development involved in outflow tract morphogenesis Source: BHF-UCL
Cardiac neural crest cell migration involved in outflow tract morphogenesis Source: Ensembl
Cardiac right ventricle formation Source: Ensembl
Cartilage morphogenesis Source: Ensembl
Cell proliferation involved in outflow tract morphogenesis Source: Ensembl
Cellular response to organic cyclic compound Source: Ensembl
Cellular response to retinoic acid Source: Ensembl
Coronary artery morphogenesis Source: Ensembl
Developmental process Source: GO_Central
Embryonic digit morphogenesis Source: Ensembl
Embryonic skeletal system development Source: Ensembl
Heart development Source: GO_Central
Heart looping Source: Ensembl
In utero embryonic development Source: Ensembl
Mesenchymal cell proliferation Source: Ensembl
Negative regulation of cardiac muscle cell apoptotic process Source: BHF-UCL
Negative regulation of DNA binding Source: Ensembl
Negative regulation of DNA-binding transcription factor activity Source: Ensembl
Negative regulation of gene expression Source: BHF-UCL
Negative regulation of osteoblast differentiation Source: Ensembl
Noradrenergic neuron differentiation Source: BHF-UCL
Odontogenesis of dentin-containing tooth Source: Ensembl
Peripheral nervous system neuron development Source: Ensembl
Positive regulation of cardiac muscle hypertrophy Source: BHF-UCL
Positive regulation of ERK1 and ERK2 cascade Source: BHF-UCL
Positive regulation of gene expression Source: BHF-UCL
Positive regulation of p38MAPK cascade Source: BHF-UCL
Positive regulation of semaphorin-plexin signaling pathway involved in outflow tract morphogenesis Source: BHF-UCL
Positive regulation of transcription by RNA polymerase II Source: UniProtKB
Positive regulation of transcription from RNA polymerase II promoter involved in norepinephrine biosynthetic process Source: BHF-UCL
Positive regulation of transcription regulatory region DNA binding Source: BHF-UCL
Primary palate development Source: Ensembl
Regulation of secondary heart field cardioblast proliferation Source: BHF-UCL
Regulation of tissue remodeling Source: BHF-UCL
Regulation of transcription by RNA polymerase II Source: GO_Central
Suckling behavior Source: Ensembl
Sympathetic nervous system development Source: Ensembl
Thymus development Source: BHF-UCL
Tongue development Source: Ensembl
Visceral serous pericardium development Source: Ensembl
Cellular Location
Nucleus

Prummel, K. D., Crowell, H. L., Nieuwenhuize, S., Brombacher, E. C., Daetwyler, S., Soneson, C., ... & Mosimann, C. (2022). Hand2 delineates mesothelium progenitors and is reactivated in mesothelioma. Nature communications, 13(1), 1677.

Huang, Z., Wang, Z., Xia, H., Ge, Z., Yu, L., Li, J., ... & Xu, Y. (2022). Long noncoding RNA HAND2-AS1: A crucial regulator of malignancy. Clinica Chimica Acta.

Gu, X., Zheng, Q., Chu, Q., & Zhu, H. (2021). HAND2-AS1: A functional cancer-related long non-coding RNA. Biomedicine & Pharmacotherapy, 137, 111317.

Marinić, M., Mika, K., Chigurupati, S., & Lynch, V. J. (2021). Evolutionary transcriptomics implicates HAND2 in the origins of implantation and regulation of gestation length. Elife, 10, e61257.

Da, C. M., Cheng, Z. Y., Gong, C. Y., Nan, W., Zhou, K. S., Zhao, G. H., & Zhang, H. H. (2020). Role of HAND2-AS1 in human tumors. Clinica Chimica Acta, 511, 189-197.

Murata, H., Tanaka, S., Tsuzuki-Nakao, T., Kido, T., Kakita-Kobayashi, M., Kida, N., ... & Okada, H. (2020). The transcription factor HAND2 up-regulates transcription of the IL15 gene in human endometrial stromal cells. Journal of Biological Chemistry, 295(28), 9596-9605.

Wang, Y., Zhu, P., Luo, J., Wang, J., Liu, Z., Wu, W., ... & Fan, Z. (2019). LncRNA HAND2‐AS1 promotes liver cancer stem cell self‐renewal via BMP signaling. The EMBO journal, 38(17), e101110.

Han, X., Zhang, J., Liu, Y., Fan, X., Ai, S., Luo, Y., ... & Shen, X. (2019). The lncRNA Hand2os1/Uph locus orchestrates heart development through regulation of precise expression of Hand2. Development, 146(13), dev176198.

Zhou, J., Lin, J., Zhang, H., Zhu, F., & Xie, R. (2018). LncRNA HAND2-AS1 sponging miR-1275 suppresses colorectal cancer progression by upregulating KLF14. Biochemical and biophysical research communications, 503(3), 1848-1853.

Yang, X., Wang, C. C., Lee, W. Y. W., Trovik, J., Chung, T. K. H., & Kwong, J. (2018). Long non-coding RNA HAND2-AS1 inhibits invasion and metastasis in endometrioid endometrial carcinoma through inactivating neuromedin U. Cancer letters, 413, 23-34.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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