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Mouse Anti-HAT1 Recombinant Antibody (EG1477) (CBMAB-EN1765-LY)

The product is antibody recognizes HAT1. The antibody EG1477 immunoassay techniques such as: ELISA, WB.
See all HAT1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
EG1477
Antibody Isotype
IgG
Application
ELISA, WB

Basic Information

Immunogen
Recombinant fragment of Human HAT1 (N-terminus).
Specificity
Human
Antibody Isotype
IgG
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Histone Acetyltransferase 1
Introduction
The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jun 2009]
Entrez Gene ID
8520
UniProt ID
O14929
Alternative Names
Histone Acetyltransferase 1; KAT1; Histone Acetyltransferase Type B Catalytic Subunit; EC 2.3.1.48;
Function
Histone acetyltransferase that plays a role in different biological processes including cell cycle progression, glucose metabolism, histone production or DNA damage repair (PubMed:31278053, PubMed:20953179, PubMed:23653357, PubMed:32081014).

Coordinates histone production and acetylation via H4 promoter binding (PubMed:31278053).

Acetylates histone H4 at 'Lys-5' (H4K5ac) and 'Lys-12' (H4K12ac) and, to a lesser extent, histone H2A at 'Lys-5' (H2AK5ac) (PubMed:22615379, PubMed:11585814).

Drives H4 production by chromatin binding to support chromatin replication and acetylation. Since transcription of H4 genes is tightly coupled to S-phase, plays an important role in S-phase entry and progression (PubMed:31278053).

Promotes homologous recombination in DNA repair by facilitating histone turnover and incorporation of acetylated H3.3 at sites of double-strand breaks (PubMed:23653357).

In addition, acetylates other substrates such as chromatin-related proteins (PubMed:32081014).

Acetylates also RSAD2 which mediates the interaction of ubiquitin ligase UBE4A with RSAD2 leading to RSAD2 ubiquitination and subsequent degradation (PubMed:31812350).

(Microbial infection) Contributes to hepatitis B virus (HBV) replication by acetylating histone H4 at the sites of 'Lys-5' and 'Lys-12' on the covalently closed circular DNA (cccDNA) minichromosome leading to its accumulation within the host cell.
Biological Process
DNA packaging Source: ProtInc
DNA replication-dependent chromatin assembly Source: UniProtKB
DNA replication-independent chromatin assembly Source: UniProtKB
Histone H4 acetylation Source: UniProtKB
Internal protein amino acid acetylation Source: ProtInc
Cellular Location
Isoform A: Nucleus matrix; Mitochondrion
Isoform B: Nucleus; Nucleus matrix; Nucleoplasm; Cytoplasm. Localization is predominantly nuclear in normal cells. Treatment with hydrogen peroxide or ionizing radiation enhances nuclear localization through redistribution of existing protein.
PTM
Phosphorylated by AMPK at Ser-190; phosphorylation increases HAT1 activity.

Kumar, N., Mondal, M., Arathi, B. P., Sundaresan, N. R., & Somasundaram, K. (2023). Histone acetyltransferase 1 (HAT1) acetylates hypoxia-inducible factor 2 alpha (HIF2A) to execute hypoxia response. Biochimica et Biophysica Acta (BBA)-Gene Regulatory Mechanisms, 1866(1), 194900.

Poziello, A., Nebbioso, A., Stunnenberg, H. G., Martens, J. H., Carafa, V., & Altucci, L. (2021). Recent insights into Histone Acetyltransferase-1: biological function and involvement in pathogenesis. Epigenetics, 16(8), 838-850.

Yang, G., Yuan, Y., Yuan, H., Wang, J., Yun, H., Geng, Y., ... & Zhang, X. (2021). Histone acetyltransferase 1 is a succinyltransferase for histones and non‐histones and promotes tumorigenesis. EMBO reports, 22(2), e50967.

Hong, Z., Xiang, Z., Zhang, P., Wu, Q., Xu, C., Wang, X., ... & Wu, D. (2021). Histone acetyltransferase 1 upregulates androgen receptor expression to modulate CRPC cell resistance to enzalutamide. Clinical and Translational Medicine, 11(7), e495.

Sun, Y., Ren, D., Zhou, Y., Shen, J., Wu, H., & Jin, X. (2021). Histone acetyltransferase 1 promotes gemcitabine resistance by regulating the PVT1/EZH2 complex in pancreatic cancer. Cell Death & Disease, 12(10), 878.

Garcia, P. A. A., Lovejoy, C. M., Nagarajan, P., Park, D., Popova, L. V., Freitas, M. A., & Parthun, M. R. (2020). Histone acetyltransferase 1 is required for DNA replication fork function and stability. Journal of Biological Chemistry, 295(25), 8363-8373.

Fan, P., Zhao, J., Meng, Z., Wu, H., Wang, B., Wu, H., & Jin, X. (2019). Overexpressed histone acetyltransferase 1 regulates cancer immunity by increasing programmed death-ligand 1 expression in pancreatic cancer. Journal of Experimental & Clinical Cancer Research, 38(1), 1-12.

Nagarajan, P., Agudelo Garcia, P. A., Iyer, C. C., Popova, L. V., Arnold, W. D., & Parthun, M. R. (2019). Early‐onset aging and mitochondrial defects associated with loss of histone acetyltransferase 1 (Hat1). Aging cell, 18(5), e12992.

Xia, P., Gu, R., Zhang, W., Shao, L., Li, F., Wu, C., & Sun, Y. (2019). MicroRNA‐377 exerts a potent suppressive role in osteosarcoma through the involvement of the histone acetyltransferase 1‐mediated Wnt axis. Journal of Cellular Physiology, 234(12), 22787-22798.

Ngo, L., Brown, T., & Zheng, Y. G. (2019). Bisubstrate inhibitors to target histone acetyltransferase 1. Chemical biology & drug design, 93(5), 865-873.

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For research use only. Not intended for any clinical use.

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