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Rabbit Anti-HIKESHI Recombinant Antibody (CBWJC-3765) (CBMAB-C5031WJ)

This product is a Rabbit antibody that recognizes HIKESHI. This antibody CBWJC-3765 can be used for immunoassay techniques such as: WB, IP, IHC-P, ICC.
See all HIKESHI antibodies

Summary

Host Animal
Rabbit
Specificity
Mouse, Human
Clone
CBWJC-3765
Antibody Isotype
IgG
Application
WB, IP, IHC-P, ICC

Basic Information

Specificity
Mouse, Human
Antibody Isotype
IgG
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Concentration
0.801 mg/mL
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Hikeshi, Heat Shock Protein Nuclear Import Factor
Introduction
This gene encodes an evolutionarily conserved nuclear transport receptor that mediates heat-shock-induced nuclear import of 70 kDa heat-shock proteins (Hsp70s) through interactions with FG-nucleoporins. The protein mediates transport of the ATP form but not the ADP form of Hsp70 proteins under conditions of heat shock stress. Structural analyses demonstrate that the protein forms an asymmetric homodimer and that the N-terminal domain consists of a jelly-roll/beta-sandwich fold structure that contains hydrophobic pockets involved in FG-nucleoporin recognition. Reduction of RNA expression levels in HeLa cells using small interfering RNAs results in inhibition of heat shock-induced nuclear accumulation of Hsp70s, indicating a role for this gene in regulation of Hsp70 nuclear import during heat shock stress. [provided by RefSeq, Apr 2016]
Entrez Gene ID
Human51501
Mouse67669
UniProt ID
HumanQ53FT3
MouseQ9DD02
Alternative Names
Hikeshi, Heat Shock Protein Nuclear Import Factor; C11orf73; Chromosome 11 Open Reading Frame 73; Lethal, Chr 7, Rinchik 6; Protein Hikeshi; HSPC138;
Function
Acts as a specific nuclear import carrier for HSP70 proteins following heat-shock stress: acts by mediating the nucleoporin-dependent translocation of ATP-bound HSP70 proteins into the nucleus. HSP70 proteins import is required to protect cells from heat shock damages. Does not translocate ADP-bound HSP70 proteins into the nucleus.
Biological Process
Cellular response to heat Source: UniProtKB
Golgi organization Source: Ensembl
Lung development Source: Ensembl
Protein import into nucleus Source: UniProtKB
Protein transport Source: UniProtKB
Cellular Location
Nucleus; Cytoplasm; Cytosol
Involvement in disease
Leukodystrophy, hypomyelinating, 13 (HLD13):
An autosomal recessive neurodegenerative disorder with infantile onset, affecting mainly the central white matter. Clinical features include early feeding difficulties, global developmental delay, postnatal progressive microcephaly, truncal hypotonia, spasticity, and variable neurologic deficits, such as visual impairment.

Nagai, T., Kawai, N., Gonda, M., Iida, K., Etani, T., Kobayashi, D., ... & Yasui, T. (2023). Role of HIKESHI on Hyperthermia for Castration-Resistant Prostate Cancer and Application of a Novel Magnetic Nanoparticle with Carbon Nanohorn for Magnetic Hyperthermia. Pharmaceutics, 15(2), 626.

Zhang, X., Xue, Z., Zhu, S., Guo, Y., Zhang, Y., Dou, J., ... & Guo, Z. (2022). Diosgenin revealed potential effect against cerebral ischemia reperfusion through HIKESHI/HSP70/NF-κB anti-inflammatory axis. Phytomedicine, 99, 153991.

Kose, S., Imai, K., Watanabe, A., Nakai, A., Suzuki, Y., & Imamoto, N. (2022). Lack of Hikeshi activates HSF1 activity under normal conditions and disturbs the heat-shock response. Life Science Alliance, 5(9).

Hattori, K., Tago, K., Memezawa, S., Ochiai, A., Sawaguchi, S., Kato, Y., ... & Yamauchi, J. (2021). The infantile leukoencephalopathy-associated mutation of C11ORF73/HIKESHI proteins generates de novo interactive activity with Filamin A, inhibiting oligodendroglial cell morphological differentiation. Medicines, 8(2), 9.

Helman, G., Zerem, A., Almad, A., Hacker, J. L., Woidill, S., Sase, S., ... & Vanderver, A. (2021). Further delineation of the clinical and pathologic features of HIKESHI-related hypomyelinating leukodystrophy. Pediatric neurology, 121, 11-19.

Tabuchi, Y., Maekawa, K., Torigoe, M., Furusawa, Y., Hirano, T., Minagawa, S., ... & Hayashi, A. (2020). HIKESHI silencing can enhance mild hyperthermia sensitivity in human oral squamous cell carcinoma HSC‑3 cells. International Journal of Molecular Medicine, 46(1), 58-66.

Imamoto, N. (2018). Heat stress-induced nuclear transport mediated by Hikeshi confers nuclear function of Hsp70s. Current Opinion in Cell Biology, 52, 82-87.

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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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