Rabbit Anti-HIKESHI Recombinant Antibody (CBWJC-3765) (CBMAB-C5031WJ)

Name: Rabbit Anti-HIKESHI Recombinant Antibody (CBWJC-3765)
SKU: CBMAB-C5031WJ
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

CBWJC-3765

Application

WB, IP, IHC-P, ICC

Specificity

Mouse, Human

Antibody Isotype

IgG

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Concentration

0.801 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Hikeshi, Heat Shock Protein Nuclear Import Factor

Introduction

This gene encodes an evolutionarily conserved nuclear transport receptor that mediates heat-shock-induced nuclear import of 70 kDa heat-shock proteins (Hsp70s) through interactions with FG-nucleoporins. The protein mediates transport of the ATP form but not the ADP form of Hsp70 proteins under conditions of heat shock stress. Structural analyses demonstrate that the protein forms an asymmetric homodimer and that the N-terminal domain consists of a jelly-roll/beta-sandwich fold structure that contains hydrophobic pockets involved in FG-nucleoporin recognition. Reduction of RNA expression levels in HeLa cells using small interfering RNAs results in inhibition of heat shock-induced nuclear accumulation of Hsp70s, indicating a role for this gene in regulation of Hsp70 nuclear import during heat shock stress. [provided by RefSeq, Apr 2016]

Entrez Gene ID

Human	51501
Mouse	67669

UniProt ID

Human	Q53FT3
Mouse	Q9DD02

Alternative Names

Hikeshi, Heat Shock Protein Nuclear Import Factor; C11orf73; Chromosome 11 Open Reading Frame 73; Lethal, Chr 7, Rinchik 6; Protein Hikeshi; HSPC138;

Function

Acts as a specific nuclear import carrier for HSP70 proteins following heat-shock stress: acts by mediating the nucleoporin-dependent translocation of ATP-bound HSP70 proteins into the nucleus. HSP70 proteins import is required to protect cells from heat shock damages. Does not translocate ADP-bound HSP70 proteins into the nucleus.

Biological Process

Cellular response to heat Source: UniProtKB
Golgi organization Source: Ensembl
Lung development Source: Ensembl
Protein import into nucleus Source: UniProtKB
Protein transport Source: UniProtKB

Cellular Location

Nucleus; Cytoplasm; Cytosol

Involvement in disease

Leukodystrophy, hypomyelinating, 13 (HLD13):
An autosomal recessive neurodegenerative disorder with infantile onset, affecting mainly the central white matter. Clinical features include early feeding difficulties, global developmental delay, postnatal progressive microcephaly, truncal hypotonia, spasticity, and variable neurologic deficits, such as visual impairment.

Nagai, T., Kawai, N., Gonda, M., Iida, K., Etani, T., Kobayashi, D., ... & Yasui, T. (2023). Role of HIKESHI on Hyperthermia for Castration-Resistant Prostate Cancer and Application of a Novel Magnetic Nanoparticle with Carbon Nanohorn for Magnetic Hyperthermia. Pharmaceutics, 15(2), 626.

Zhang, X., Xue, Z., Zhu, S., Guo, Y., Zhang, Y., Dou, J., ... & Guo, Z. (2022). Diosgenin revealed potential effect against cerebral ischemia reperfusion through HIKESHI/HSP70/NF-κB anti-inflammatory axis. Phytomedicine, 99, 153991.

Kose, S., Imai, K., Watanabe, A., Nakai, A., Suzuki, Y., & Imamoto, N. (2022). Lack of Hikeshi activates HSF1 activity under normal conditions and disturbs the heat-shock response. Life Science Alliance, 5(9).

Hattori, K., Tago, K., Memezawa, S., Ochiai, A., Sawaguchi, S., Kato, Y., ... & Yamauchi, J. (2021). The infantile leukoencephalopathy-associated mutation of C11ORF73/HIKESHI proteins generates de novo interactive activity with Filamin A, inhibiting oligodendroglial cell morphological differentiation. Medicines, 8(2), 9.

Helman, G., Zerem, A., Almad, A., Hacker, J. L., Woidill, S., Sase, S., ... & Vanderver, A. (2021). Further delineation of the clinical and pathologic features of HIKESHI-related hypomyelinating leukodystrophy. Pediatric neurology, 121, 11-19.

Tabuchi, Y., Maekawa, K., Torigoe, M., Furusawa, Y., Hirano, T., Minagawa, S., ... & Hayashi, A. (2020). HIKESHI silencing can enhance mild hyperthermia sensitivity in human oral squamous cell carcinoma HSC‑3 cells. International Journal of Molecular Medicine, 46(1), 58-66.

Imamoto, N. (2018). Heat stress-induced nuclear transport mediated by Hikeshi confers nuclear function of Hsp70s. Current Opinion in Cell Biology, 52, 82-87.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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