Mouse Anti-HLA-DMB (AA 22-129) Recombinant Antibody (CBFYH-1324) (CBMAB-H2290-FY)

Name: Mouse Anti-HLA-DMB (AA 22-129) Recombinant Antibody (CBFYH-1324)
SKU: CBMAB-H2290-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Basic Information

Host Animal

Mouse

Clone

CBFYH-1324

Application

ELISA, IP, WB

Immunogen

Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Immunogen sequence: VAHVESTCLL DDAGTPKDFT YCISFNKDLL TCWDPEENKM APCEFGVLNS LANVLSQHLN QKDTLMQRLR NGLQNCATHT QPFWGSLTNR TRPPSVQVAK TTPFNTRE

Specificity

Human

Antibody Isotype

IgG2a, κ

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Epitope

AA 22-129

More Infomation

Target

Full Name

major histocompatibility complex, class II, DM beta

Introduction

HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.

Entrez Gene ID

3109

UniProt ID

P28068

Alternative Names

Major Histocompatibility Complex, Class II, DM Beta; Really Interesting New Gene 7 Protein; MHC Class II Antigen DMB; RING7; HLA Class II Histocompatibility Antigen, DM Beta Chain; Class II Histocompatibility Antigen, M Beta Chain

Function

Plays a critical role in catalyzing the release of class II-associated invariant chain peptide (CLIP) from newly synthesized MHC class II molecules and freeing the peptide binding site for acquisition of antigenic peptides. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO.

Biological Process

Adaptive immune response Source: UniProtKB-KW
Antigen processing and presentation of exogenous peptide antigen via MHC class II Source: UniProtKB
MHC class II protein complex assembly Source: UniProtKB
Peptide antigen assembly with MHC class II protein complex Source: UniProtKB
Positive regulation of T cell activation Source: GO_Central
Positive regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell Source: UniProtKB
Positive regulation of T cell proliferation Source: UniProtKB

Cellular Location

Lysosome membrane; Late endosome membrane. Localizes to late endocytic compartment. Associates with lysosome membranes.

Topology

Lumenal: 19-218
Helical: 219-239
Cytoplasmic: 240-263

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Mouse Anti-HLA-DMB Recombinant Antibody (CBFYH-1327) (CAT#: CBMAB-H2293-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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