Mouse Anti-HMBOX1 Monoclonal Antibody (CBFYH-3156) (CBMAB-H2444-FY)

Name: Mouse Anti-HMBOX1 Monoclonal Antibody (CBFYH-3156)
SKU: CBMAB-H2444-FY
Rating: 5 (1 reviews)

See all HMBOX1 antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYH-3156

Application

Dot

Immunogen

HMBOX1 antibody was raised in mouse using recombinant Human Homeobox Containing 1. Immunogen type: Recombinant protein

Specificity

Human

Antibody Isotype

IgG1

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Concentration

0.1 mg/mL

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Homeobox Containing 1

Introduction

HMBOX1 (Homeobox Containing 1) is a Protein Coding gene.

Entrez Gene ID

79618

UniProt ID

Q6NT76

Alternative Names

Homeobox Containing 1; Homeobox Telomere-Binding Protein 1; Homeobox-Containing Protein PBHNF; Homeobox-Containing Protein 1; HNF1LA; PBHNF; HOT1

Function

Binds directly to 5'-TTAGGG-3' repeats in telomeric DNA (PubMed:23813958, PubMed:23685356).

Associates with the telomerase complex at sites of active telomere processing and positively regulates telomere elongation (PubMed:23685356).

Important for TERT binding to chromatin, indicating a role in recruitment of the telomerase complex to telomeres (By similarity).

Also plays a role in the alternative lengthening of telomeres (ALT) pathway in telomerase-negative cells where it promotes formation and/or maintenance of ALT-associated promyelocytic leukemia bodies (APBs) (PubMed:23813958).

Enhances formation of telomere C-circles in ALT cells, suggesting a possible role in telomere recombination (PubMed:23813958).

Might also be involved in the DNA damage response at telomeres (PubMed:23813958).

Biological Process

Negative regulation of transcription, DNA-templated Source: UniProtKB
Negative regulation of transcription by RNA polymerase II Source: NTNU_SB
Positive regulation of chromatin binding Source: BHF-UCL
Positive regulation of telomerase activity Source: BHF-UCL
Positive regulation of telomere maintenance via telomerase Source: BHF-UCL
Positive regulation of transcription, DNA-templated Source: InterPro
Regulation of telomerase activity Source: BHF-UCL

Cellular Location

Cytoplasm; Nucleus; Cajal body; PML body; Telomere. Predominantly detected in cytoplasm (PubMed:16825764, PubMed:19728927). Localizes in a dynamic manner to actively processed telomeres (PubMed:23685356). Localizes to the periphery of Cajal bodies (PubMed:23685356). Associates with PML nuclear bodies in telomerase-negative cells (PubMed:23813958).
Isoform 5: Cytoplasm; Nucleus

Jiang, Y., Mu, H., & Zhao, H. (2023). HMBOX1, a member of the homeobox family: current research progress. Central European Journal of Immunology, 48(1).

Jiang, W., Jiang, Y., Zhang, X., Mu, H., Song, Y., & Zhao, H. (2023). Metabolomic analysis reveals the influence of HMBOX1 on RAW264. 7 cells proliferation based on UPLC-MS/MS. BMC genomics, 24(1), 272.

Zhu, X., Fu, H., Sun, J., Di, Q., & Xu, Q. (2022). N6-methyladenosine modification on Hmbox1 is related to telomere dysfunction in DEHP-induced male reproductive injury. Life Sciences, 309, 121005.

Nie, M., Li, H., Liu, P., & Dang, P. (2022). HMBOX1 attenuates LPS‑induced periodontal ligament stem cell injury by inhibiting CXCL10 expression through the NF‑κB signaling pathway. Experimental and Therapeutic Medicine, 23(3), 1-10.

Chen, S., Li, Y., Zhi, S., Ding, Z., Wang, W., Peng, Y., ... & Li, J. (2020). WTAP promotes osteosarcoma tumorigenesis by repressing HMBOX1 expression in an m6A-dependent manner. Cell death & disease, 11(8), 659.

Ma, H., Su, L., He, X., & Miao, J. (2019). Loss of HMBOX1 promotes LPS-induced apoptosis and inhibits LPS-induced autophagy of vascular endothelial cells in mouse. Apoptosis, 24, 946-957.

Diao, N., Li, Y., Yang, J., Jin, C., Meng, X., Jiao, W., ... & Lu, N. (2019). High expression of HMBOX1 contributes to poor prognosis of gastric cancer by promoting cell proliferation and migration. Biomedicine & Pharmacotherapy, 115, 108867.

Zhao, H., Han, Q., Lu, N., Xu, D., Tian, Z., & Zhang, J. (2018). HMBOX1 in hepatocytes attenuates LPS/D-GalN-induced liver injury by inhibiting macrophage infiltration and activation. Molecular immunology, 101, 303-311.

Yu, Y. L., Diao, N. N., Li, Y. Z., Meng, X. H., Jiao, W. L., Feng, J. B., ... & Lu, N. (2018). Low expression level of HMBOX1 in high-grade serous ovarian cancer accelerates cell proliferation by inhibiting cell apoptosis. Biochemical and Biophysical Research Communications, 501(2), 380-386.

Zhou, J., Wang, M., & Deng, D. (2018). c-Fos/microRNA-18a feedback loop modulates the tumor growth via HMBOX1 in human gliomas. Biomedicine & Pharmacotherapy, 107, 1705-1711.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Mouse Anti-HMBOX1 Monoclonal Antibody (CBFYH-3156)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry