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Mouse Anti-ARHGAP45 Recombinant Antibody (4H7) (CBMAB-A3976-LY)

The product is antibody recognizes HMHA1. The antibody 4H7 immunoassay techniques such as: WB, ELISA.
See all ARHGAP45 antibodies

Summary

Host Animal
Mouse
Specificity
Human, Mouse
Clone
4H7
Antibody Isotype
IgG2a, κ
Application
WB, ELISA

Basic Information

Immunogen
HMHA1 (AAH65223.1, 91 a.a. ~ 190 a.a) partial recombinant protein with GST tag.
Specificity
Human, Mouse
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.4
Preservative
None
Concentration
Batch dependent
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
Rho GTPase Activating Protein 45
Entrez Gene ID
Human23526
Mouse70719
UniProt ID
HumanQ92619
MouseQ3TBD2
Alternative Names
HA-1; HLA-HA1; KIAA0223
Function
Contains a GTPase activator for the Rho-type GTPases (RhoGAP) domain that would be able to negatively regulate the actin cytoskeleton as well as cell spreading. However, also contains N-terminally a BAR-domin which is able to play an autoinhibitory effect on this RhoGAP activity.
Precursor of the histocompatibility antigen HA-1. More generally, minor histocompatibility antigens (mHags) refer to immunogenic peptide which, when complexed with MHC, can generate an immune response after recognition by specific T-cells. The peptides are derived from polymorphic intracellular proteins, which are cleaved by normal pathways of antigen processing. The binding of these peptides to MHC class I or class II molecules and its expression on the cell surface can stimulate T-cell responses and thereby trigger graft rejection or graft-versus-host disease (GVHD) after hematopoietic stem cell transplantation from HLA-identical sibling donor. GVHD is a frequent complication after bone marrow transplantation (BMT), due to mismatch of minor histocompatibility antigen in HLA-matched sibling marrow transplants. Specifically, mismatching for mHag HA-1 which is recognized as immunodominant, is shown to be associated with the development of severe GVHD after HLA-identical BMT. HA-1 is presented to the cell surface by MHC class I HLA-A*0201, but also by other HLA-A alleles. This complex specifically elicits donor-cytotoxic T-lymphocyte (CTL) reactivity against hematologic malignancies after treatment by HLA-identical allogenic BMT. It induces cell recognition and lysis by CTL.
Biological Process
Activation of GTPase activity Source: GO_Central
Intracellular signal transduction Source: InterPro
Neutrophil degranulation Source: Reactome
Regulation of small GTPase mediated signal transduction Source: Reactome
Cellular Location
Cytoplasm; Ruffle membrane
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For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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