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Mouse Anti-HOMER1 Recombinant Antibody (2F8) (CBMAB-A4001-LY)

The product is antibody recognizes HOMER1. The antibody 2F8 immunoassay techniques such as: WB, ELISA.
See all HOMER1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
2F8
Antibody Isotype
IgG2b, κ
Application
WB, ELISA

Basic Information

Immunogen
HOMER1 (AAH15502, 1 a.a. ~ 354 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Specificity
Human
Antibody Isotype
IgG2b, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Purity
> 95% Purity determined by SDS-PAGE.
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

Target

Full Name
homer homolog 1 (Drosophila)
Introduction
This gene encodes a member of the homer family of dendritic proteins. Members of this family regulate group 1 metabotrophic glutamate receptor function. [provided by RefSeq]
Entrez Gene ID
9456
UniProt ID
Q86YM7
Alternative Names
HOMER; HOMER1A; HOMER1B; HOMER1C; SYN47; Ves-1
Function
Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. Forms a high-order complex with SHANK1, which in turn is necessary for the structural and functional integrity of dendritic spines (By similarity).

Negatively regulates T cell activation by inhibiting the calcineurin-NFAT pathway. Acts by competing with calcineurin/PPP3CA for NFAT protein binding, hence preventing NFAT activation by PPP3CA (PubMed:18218901).
Biological Process
Behavioral response to cocaine Source: Ensembl
Chemical homeostasis within a tissue Source: Ensembl
Chemical synaptic transmission Source: ProtInc
Circadian rhythm Source: Ensembl
G protein-coupled glutamate receptor signaling pathway Source: GO_Central
Phospholipase C-activating G protein-coupled glutamate receptor signaling pathway Source: ProtInc
Positive regulation of calcium ion transport Source: Ensembl
Positive regulation of signal transduction Source: BHF-UCL
Protein localization to synapse Source: Ensembl
Protein tetramerization Source: UniProtKB
Regulation of calcium ion import Source: Ensembl
Regulation of cation channel activity Source: BHF-UCL
Regulation of dendritic spine maintenance Source: UniProtKB
Regulation of postsynaptic neurotransmitter receptor activity Source: Ensembl
Regulation of store-operated calcium entry Source: GO_Central
Regulation of synaptic transmission, glutamatergic Source: UniProtKB
Response to calcium ion Source: BHF-UCL
Response to nicotine Source: Ensembl
Skeletal muscle contraction Source: Ensembl
Skeletal muscle fiber development Source: Ensembl
Cellular Location
Cytoplasm; Postsynaptic density; Synapse; Dendritic spine. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum.

de Bartolomeis, A., Barone, A., Buonaguro, E. F., Tomasetti, C., Vellucci, L., & Iasevoli, F. (2022). The Homer1 family of proteins at the crossroad of dopamine-glutamate signaling: An emerging molecular “Lego” in the pathophysiology of psychiatric disorders. A systematic review and translational insight. Neuroscience & Biobehavioral Reviews, 136, 104596.

Fei, X., Dou, Y. N., Wang, L., Wu, X., Huan, Y., Wu, S., ... & Fei, Z. (2022). Homer1 promotes the conversion of A1 astrocytes to A2 astrocytes and improves the recovery of transgenic mice after intracerebral hemorrhage. Journal of Neuroinflammation, 19(1), 1-18.

Liu, C. Y., Chen, J. B., Liu, Y. Y., Zhou, X. M., Zhang, M., Jiang, Y. M., ... & Chen, J. X. (2022). Saikosaponin D exerts antidepressant effect by regulating Homer1-mGluR5 and mTOR signaling in a rat model of chronic unpredictable mild stress. Chinese Medicine, 17(1), 60.

Yoon, S., Piguel, N. H., Khalatyan, N., Dionisio, L. E., Savas, J. N., & Penzes, P. (2021). Homer1 promotes dendritic spine growth through ankyrin-G and its loss reshapes the synaptic proteome. Molecular psychiatry, 26(6), 1775-1789.

Urdánoz-Casado, A., Sánchez-Ruiz de Gordoa, J., Robles, M., Acha, B., Roldan, M., Zelaya, M. V., ... & Mendioroz, M. (2021). Gender-dependent deregulation of linear and circular RNA variants of HOMER1 in the entorhinal cortex of Alzheimer’s disease. International Journal of Molecular Sciences, 22(17), 9205.

Reshetnikov, V. V., & Bondar, N. P. (2021). The role of stress-induced changes of Homer1 expression in stress susceptibility. Biochemistry (Moscow), 86(6), 613-626.

Zhao, M., Dong, G., Meng, Q., Lin, S., & Li, X. (2020). Circ‐HOMER1 enhances the inhibition of miR‐1322 on CXCL6 to regulate the growth and aggressiveness of hepatocellular carcinoma cells. Journal of Cellular Biochemistry, 121(11), 4440-4449.

Li, M. X., Li, Q., Sun, X. J., Luo, C., Li, Y., Wang, Y. N., ... & Chen, H. (2019). Increased Homer1-mGluR5 mediates chronic stress-induced depressive-like behaviors and glutamatergic dysregulation via activation of PERK-eIF2α. Progress in Neuro-Psychopharmacology and Biological Psychiatry, 95, 109682.

Rybchyn, M. S., Islam, K. S., Brennan-Speranza, T. C., Cheng, Z., Brennan, S. C., Chang, W., ... & Conigrave, A. D. (2019). Homer1 mediates CaSR-dependent activation of mTOR complex 2 and initiates a novel pathway for AKT-dependent β-catenin stabilization in osteoblasts. Journal of Biological Chemistry, 294(44), 16337-16350.

Benedetti, F., Poletti, S., Locatelli, C., Mazza, E., Lorenzi, C., Vitali, A., ... & Colombo, C. (2018). A Homer 1 gene variant influences brain structure and function, lithium effects on white matter, and antidepressant response in bipolar disorder: a multimodal genetic imaging study. Progress in Neuro-Psychopharmacology and Biological Psychiatry, 81, 88-95.

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For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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