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Mouse Anti-HOOK1 Recombinant Antibody (CBFYH-1694) (CBMAB-H2701-FY)

This product is mouse antibody that recognizes HOOK1. The antibody CBFYH-1694 can be used for immunoassay techniques such as: ELISA, IHC-P, WB.
See all HOOK1 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYH-1694
Antibody Isotype
IgG2a, κ
Application
ELISA, IHC-P, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
Hook Microtubule Tethering Protein 1
Introduction
This gene encodes a member of the hook family of coiled-coil proteins, which bind to microtubules and organelles through their N- and C-terminal domains, respectively. The encoded protein localizes to discrete punctuate subcellular structures, and interacts with several members of the Rab GTPase family involved in endocytosis. It is thought to link endocytic membrane trafficking to the microtubule cytoskeleton. Several alternatively spliced transcript variants have been identified, but the full-length nature of some of these variants has not been determined.
Entrez Gene ID
51361
UniProt ID
Q9UJC3
Alternative Names
Hook Microtubule Tethering Protein 1; H-Hook1; HHK1; Hook Homolog 1 (Drosophila); Protein Hook Homolog 1; Hook Homolog 1; HK1
Function
Component of the FTS/Hook/FHIP complex (FHF complex) (PubMed:18799622, PubMed:32073997).

The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex) (PubMed:18799622).

FHF complex promotes the distribution of AP-4 complex to the perinuclear area of the cell (PubMed:32073997).

Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton (By similarity).
Biological Process
Cytoplasmic microtubule organization Source: GO_Central
Cytoskeleton-dependent intracellular transport Source: GO_Central
Early endosome to late endosome transport Source: UniProtKB
Endosome organization Source: UniProtKB
Endosome to lysosome transport Source: UniProtKB
Golgi organization Source: Ensembl
Lysosome organization Source: UniProtKB
Manchette assembly Source: Ensembl
Protein localization to perinuclear region of cytoplasm Source: UniProtKB
Protein transport Source: UniProtKB-KW
Cellular Location
Cytoskeleton; Cytoplasm. Localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, multivesicular bodies (MVBs), lysosomes, or mitochondria (By similarity). Often found in close association with microtubules (By similarity). Does not associate with the Golgi complex. During spermiogenesis, it localizes to the manchette in spermatids from steps 8-10. It is also present between the microtubule manchette and the nucleus. During manchette elongation, it is preferentially localized to the nuclear ring of the manchette, whereas the strong localization to the manchette decreases. In more mature spermatids, while the manchette migrates posteriorly, it localizes to punctuates spots. At later stages of spermatid differentiation, the punctuate expression pattern is found at both the attachment site and the proximal end of the elongated manchette. In contrast, it is not present in mature spermatozoa (By similarity).

Yin, L., Li, W., Chen, X., Wang, R., Zhang, T., Meng, J., ... & Yang, H. (2023). HOOK1 Inhibits the Progression of Renal Cell Carcinoma via TGF‐β and TNFSF13B/VEGF‐A Axis. Advanced Science, 2206955.

Higashi, S., Makiyama, T., Sakane, H., Nogami, S., & Shirataki, H. (2022). Regulation of Hook1-mediated endosomal sorting of clathrin-independent cargo by γ-taxilin. Journal of Cell Science, 135(1), jcs258849.

Yang, H., He, L., Zhang, Y., Li, Y., Huang, X., Li, Y., ... & Wang, L. (2020). The clinicopathological and prognostic implications of tyrosine phosphatase SHP2 and ankyrin Hook1 gene expression in non-small cell lung cancer patients treated with gemcitabine plus platinum as first-line chemotherapy. Annals of Palliative Medicine, 9(5), 2943952-2942952.

Olenick, M. A., Dominguez, R., & Holzbaur, E. L. (2019). Dynein activator Hook1 is required for trafficking of BDNF-signaling endosomes in neurons. Journal of Cell Biology, 218(1), 220-233.

He, L., Li, Y., Huang, X., Cheng, H., Ke, Y., & Wang, L. (2019). The prognostic significance of SHP2 and its binding protein Hook1 in non-small cell lung cancer. OncoTargets and therapy, 12, 5897.

Xie, J. C., Ma, X. Y., Liu, X. H., Yu, J., Zhao, Y. C., Tan, Y., ... & Zhao, Y. X. (2018). Hypoxia increases amyloid-β level in exosomes by enhancing the interaction between CD147 and Hook1. American Journal of Translational Research, 10(1), 150.

Sheikh, T. I., Harripaul, R., Ayub, M., & Vincent, J. B. (2018). MeCP2 AT‐Hook1 mutations in patients with intellectual disability and/or schizophrenia disrupt DNA binding and chromatin compaction in vitro. Human Mutation, 39(5), 717-728.

Cao, J., Huang, Y. Q., Lan, X. B., & Ge, M. H. (2018). Clinicopathological and prognostic significance of SHP2 and Hook1 expression in patients with thyroid carcinoma. Human Pathology, 81, 105-112.

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For research use only. Not intended for any clinical use.

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