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Mouse Anti-HOOK3 Recombinant Antibody (CBFYH-1707) (CBMAB-H2712-FY)

This product is mouse antibody that recognizes HOOK3. The antibody CBFYH-1707 can be used for immunoassay techniques such as: ELISA, IHC-P, WB.
See all HOOK3 antibodies

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYH-1707
Antibody Isotype
IgG2a, κ
Application
ELISA, IHC-P, WB

Basic Information

Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Buffer
PBS, pH 7.2
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Target

Full Name
hook homolog 3 (Drosophila)
Introduction
Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.
Entrez Gene ID
84376
UniProt ID
Q86VS8
Alternative Names
Hook Microtubule Tethering Protein 3; H-Hook3; HHK3; Hook Homolog 3 (Drosophila); Protein Hook Homolog 3; Hook Homolog 3; HK3
Function
Probably serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking (By similarity).

Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (PubMed:25035494).

FHF complex promotes the distribution of AP-4 complex to the perinuclear area of the cell (PubMed:32073997).

(Microbial infection) May serve as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking.
Biological Process
Cytoplasmic microtubule organization Source: UniProtKB
Cytoskeleton-dependent intracellular transport Source: GO_Central
Early endosome to late endosome transport Source: UniProtKB
Endosome organization Source: UniProtKB
Endosome to lysosome transport Source: UniProtKB
Golgi localization Source: UniProtKB
Interkinetic nuclear migration Source: BHF-UCL
Lysosome organization Source: UniProtKB
Microtubule anchoring at centrosome Source: BHF-UCL
Negative regulation of neurogenesis Source: BHF-UCL
Neuronal stem cell population maintenance Source: Ensembl
Protein localization to centrosome Source: BHF-UCL
Protein localization to perinuclear region of cytoplasm Source: UniProtKB
Protein transport Source: UniProtKB-KW
Cellular Location
Cytoskeleton; Golgi apparatus. Enriched at the cis-face of the Golgi complex. Localizes to microtubule asters in prophase (PubMed:11238449). Localizes to the manchette in elongating spermatids (By similarity).

Feng, Y., Liu, B., Chen, J., Li, H., & Zhang, D. (2023). The Circ_35953 induced by the NF‐κ B mediated the septic AKI via targeting mi R‐7219‐5p/HOOK3 and IGFBP7 axis. Journal of Cellular and Molecular Medicine, 27(9), 1261-1276.

Ali, F. A., & Carter, A. (2023). Architecture of the dynein adaptor complex Fts-Hook3-FHIP1B. Biophysical Journal, 122(3), 411a.

Zhang, X., Wang, F., Yan, F., Huang, D., Wang, H., Gao, B., ... & Yan, J. (2022). Identification of a novel HOOK3-FGFR1 fusion gene involved in activation of the NF-kappaB pathway. Cancer Cell International, 22(1), 1-12.

Chen, Y., Guan, Q., Feng, X., Jiang, X., Xu, G., & Hou, H. (2021). LINC-ROR regulates myocardial ischemia/reperfusion injury via targeting of miR-129-5p/Hook3 axis. Tropical Journal of Pharmaceutical Research, 20(3), 445-451.

Sun, T., Chen, J., Sun, X., & Wang, G. (2021). Midazolam increases cisplatin-sensitivity in non-small cell lung cancer (NSCLC) via the miR-194-5p/HOOK3 axis. Cancer Cell International, 21(1), 1-11.

Wortzel, I., Maik-Rachline, G., Yadav, S. S., Hanoch, T., & Seger, R. (2021). Mitotic HOOK3 phosphorylation by ERK1c drives microtubule-dependent Golgi destabilization and fragmentation. Iscience, 24(6).

Jin, Y., & Ni, S. (2020). miR‐496 remedies hypoxia reoxygenation–induced H9c2 cardiomyocyte apoptosis via Hook3‐targeted PI3k/Akt/mTOR signaling pathway activation. Journal of Cellular Biochemistry, 121(1), 698-712.

Kendrick, A. A., Dickey, A. M., Redwine, W. B., Tran, P. T., Vaites, L. P., Dzieciatkowska, M., ... & Reck-Peterson, S. L. (2019). Hook3 is a scaffold for the opposite-polarity microtubule-based motors cytoplasmic dynein-1 and KIF1C. Journal of Cell Biology, 218(9), 2982-3001.

Siddiqui, N., Zwetsloot, A. J., Bachmann, A., Roth, D., Hussain, H., Brandt, J., ... & Straube, A. (2019). PTPN21 and Hook3 relieve KIF1C autoinhibition and activate intracellular transport. Nature communications, 10(1), 2693.

Garabedian, A., Bolufer, A., Leng, F., & Fernandez-Lima, F. (2018). Peptide sequence influence on the conformational dynamics and DNA binding of the intrinsically disordered AT-hook 3 peptide. Scientific Reports, 8(1), 10783.

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For research use only. Not intended for any clinical use.

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