Rabbit Anti-HP Recombinant Antibody (
5A5) (V2LY-0725-LY1069)

Name: Rabbit Anti-HP Recombinant Antibody (5A5)
SKU: V2LY-0725-LY1069
Rating: 5 (1 reviews)

See all HP antibodies

Compare Online Inquiry

Tested Data

Rabbit Anti-HP Recombinant Antibody (5A5) (V2LY-0725-LY1069) in IHC

IHC image of V2LY-0725-LY1069 diluted at 1:100 and staining in paraffin-embedded human placenta tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Rabbit Anti-HP Recombinant Antibody (5A5) (V2LY-0725-LY1069) in IHC

IHC image of V2LY-0725-LY1069 diluted at 1:100 and staining in paraffin-embedded human liver tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

Datasheet

SDS

Request for COA

Datasheet Target Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

5A5

Application

ELISA, IHC

Immunogen

A synthesized peptide derived from human Haptoglobin.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC	1:50-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Haptoglobin

Entrez Gene ID

3240

UniProt ID

P00738

Function

As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity, and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.

The uncleaved form of allele alpha-2 (2-2), known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens.

Biological Process

Acute inflammatory response Source: GO_Central
Acute-phase response Source: UniProtKB-KW
Defense response Source: ProtInc
Defense response to bacterium Source: UniProtKB-KW
Immune system process Source: UniProtKB-KW
Negative regulation of hydrogen peroxide catabolic process Source: BHF-UCL
Negative regulation of oxidoreductase activity Source: BHF-UCL
Positive regulation of cell death Source: BHF-UCL
Response to hydrogen peroxide Source: BHF-UCL
Zymogen activation Source: GO_Central

Cellular Location

Secreted

Involvement in disease

Anhaptoglobinemia (AHP):
A condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin. Congenital haptoglobin deficiency is a risk factor for anaphylactic non-hemolytic transfusion reactions.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Rabbit Anti-HP Recombinant Antibody (
5A5)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Related Products

Mouse Anti-HP Monoclonal Antibody (CBFYH-3196) (CAT#: CBMAB-H0804-FY)

Mouse Anti-HP Recombinant Antibody (CBFYH-1867) (CAT#: CBMAB-H2875-FY)

Mouse Anti-HP Recombinant Antibody (CBFYH-1862) (CAT#: CBMAB-H2870-FY)

Mouse Anti-HP Recombinant Antibody (CBFYH-1865) (CAT#: CBMAB-H2873-FY)

Mouse Anti-HP Monoclonal Antibody (CBFYR0663) (CAT#: CBMAB-R0663-FY)

Mouse Anti-HP Recombinant Antibody (9G10) (CAT#: CBMAB-AO571LY)

Mouse Anti-HP Recombinant Antibody (CBFYH-1870) (CAT#: CBMAB-H2878-FY)

Mouse Anti-HP Recombinant Antibody (CAP890) (CAT#: CBMAB-AP2693LY)

Mouse Anti-HP Recombinant Antibody (CBFYH-1861) (CAT#: CBMAB-H2869-FY)

Mouse Anti-HP Recombinant Antibody (CBFYH-1868) (CAT#: CBMAB-H2876-FY)

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry