Rabbit Anti-HPCA Recombinant Antibody (BA0323) (CBMAB-0364CQ)

Name: Rabbit Anti-HPCA Recombinant Antibody (BA0323)
SKU: CBMAB-0364CQ
Rating: 5 (1 reviews)

See all HPCA antibodies

Compare Online Inquiry

Datasheet

SDS

Request for COA

Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Rabbit

Clone

BA0323

Application

Immunogen

Synthetic peptide between residues at the N-terminus of Human Hippocalcin

Specificity

Human, Mouse, Rat

Antibody Isotype

IgG

Clonality

Monoclonal

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Purity

>95% as determined by analysis by SDS-PAGE

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

Hippocalcin

Introduction

The HPCA gene encodes hippocalcin, a member of a family of neuron-specific Ca(2+)-binding proteins found in the retina and brain. The protein encoded by this gene is a member of neuron-specific calcium-binding proteins family found in the retina and brain. HPCA gene contains 3 exons and spans 7 kb. It has similarities to proteins located in the photoreceptor cells that regulate photosignal transduction in a calcium-sensitive manner. This protein displays recoverin activity and a calcium-dependent inhibition of rhodopsin kinase. It is identical to the rat and mouse hippocalcin proteins and thought to play an important role in neurons of the central nervous system in a number of species.

Entrez Gene ID

Human	3208
Mouse	15444
Rat	29177

UniProt ID

Human	P84074
Mouse	P84075
Rat	P84076

Alternative Names

BDR2; DYT2

Function

Calcium-binding protein that may play a role in the regulation of voltage-dependent calcium channels (PubMed:28398555).

May also play a role in cyclic-nucleotide-mediated signaling through the regulation of adenylate and guanylate cyclases (By similarity).

Biological Process

Activation of phospholipase D activity Source: Ensembl
Brain development Source: Ensembl
Calcium-mediated signaling Source: Ensembl
Cellular response to calcium ion Source: UniProtKB
Cellular response to electrical stimulus Source: Ensembl
Cellular response to monosodium glutamate Source: Ensembl
Inner ear development Source: Ensembl
Negative regulation of guanylate cyclase activity Source: Ensembl
Positive regulation of adenylate cyclase activity Source: Ensembl
Positive regulation of protein targeting to membrane Source: Ensembl
Regulation of postsynaptic neurotransmitter receptor internalization Source: Ensembl
Regulation of voltage-gated calcium channel activity Source: UniProtKB
Response to Aroclor 1254 Source: Ensembl
Response to ketamine Source: Ensembl
Response to L-glutamate Source: Ensembl
Retina development in camera-type eye Source: Ensembl

Cellular Location

Cytosol; Membrane. Association with membranes is calcium-dependent (By similarity). Enriched in the perinuclear region, probably at the trans Golgi network in response to calcium (PubMed:28398555).

Involvement in disease

Dystonia 2, torsion, autosomal recessive (DYT2):
A form of torsion dystonia, a disease defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. 'Torsion' refers to the twisting nature of body movements, often affecting the trunk. DYT2 is a slowly progressive form that first affects distal limbs and later involves the neck, orofacial, and craniocervical regions.

PTM

Myristoylation facilitates association with membranes.

Magrinelli, F., Bhatia, K. P., Beiraghi Toosi, M., Arab, F., Karimiani, E. G., Sedighzadeh, S., ... & Maroofian, R. (2023). Childhood‐Onset Choreo‐Dystonia Due to a Recurrent Novel Homozygous Nonsense HPCA Variant: Case Series and Literature Review. Movement Disorders Clinical Practice, 10(1), 101-108.

Siegert, S., Schmidt, W. M., Pletschko, T., Bittner, R. E., Gobara, S., & Freilinger, M. (2021). Specific cognitive changes due to Hippocalcin alterations? A novel familial homozygous Hippocalcin variant associated with inherited dystonia and altered cognition. Neuropediatrics, 52(05), 377-382.

Hesketh, N. (2020). Effect of S100B Deletion on Membrane Properties and Localization of NCALD and HPCA.

Sheremet, Y., Olifirov, B., Okhrimenko, A., Cherkas, V., Bagatskaya, O., & Belan, P. (2020). Hippocalcin Distribution between the Cytosol and Plasma Membrane of Living Cells. Neurophysiology, 52, 2-13.

Osypenko, D. S., Dovgan, A. V., Kononenko, N. I., Dromaretsky, A. V., Matvieienko, M., Rybachuk, O. A., ... & Belan, P. (2019). Perturbed Ca2+-dependent signaling of DYT2 hippocalcin mutant as mechanism of autosomal recessive dystonia. Neurobiology of Disease, 132, 104529.

Kang, M. J., Park, S. Y., & Han, J. S. (2019). MicroRNA-24-3p regulates neuronal differentiation by controlling hippocalcin expression. Cellular and Molecular Life Sciences, 76, 4569-4580.

Key, J., Mueller, A. K., Gispert, S., Matschke, L., Wittig, I., Corti, O., ... & Auburger, G. (2019). Ubiquitylome profiling of Parkin-null brain reveals dysregulation of calcium homeostasis factors ATP1A2, Hippocalcin and GNA11, reflected by altered firing of noradrenergic neurons. Neurobiology of disease, 127, 114-130.

Atasu, B., Hanagasi, H., Bilgic, B., Pak, M., Erginel‐Unaltuna, N., Hauser, A. K., ... & Lohmann, E. (2018). HPCA confirmed as a genetic cause of DYT2‐like dystonia phenotype. Movement Disorders, 33(8), 1354-1358.

Krishnan, A., & Venkataraman PhD, V. (2018). Multifaceted neuronal calcium sensor proteins: NCALD, HPCA & S100B.

Ask a question We look forward to hearing from you.

0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Have you used Rabbit Anti-HPCA Recombinant Antibody (BA0323)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon

$30 eGift Card
Submit a review

Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Online Inquiry

Request bulk or custom quote Second antibody and isotype control

Contact us

Tel: (USA)
(UK)
Fax:

Global Locations

Email:

Online Inquiry