Mouse Anti-HS2ST1 Recombinant Antibody (CBFYH-1975) (CBMAB-H2986-FY)

Name: Mouse Anti-HS2ST1 Recombinant Antibody (CBFYH-1975)
SKU: CBMAB-H2986-FY
Rating: 5 (1 reviews)

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Datasheet

SDS

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Datasheet Target References Q & As Review & reward Protocols Associated Products

Basic Information

Host Animal

Mouse

Clone

CBFYH-1975

Application

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Heparan Sulfate 2-O-Sulfotransferase 1/HS2ST1, Met59-Asn356

Specificity

Human

Antibody Isotype

IgG1

Application Notes

The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Lyophilized

Buffer

PBS

Concentration

LYOPH

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

More Infomation

Target

Full Name

heparan sulfate 2-O-sulfotransferase 1

Introduction

Heparan sulfate biosynthetic enzymes are key components in generating a myriad of distinct heparan sulfate fine structures that carry out multiple biologic activities. This gene encodes a member of the heparan sulfate biosynthetic enzyme family that transfers sulfate to the 2 position of the iduronic acid residue of heparan sulfate. The disruption of this gene resulted in no kidney formation in knockout embryonic mice, indicating that the absence of this enzyme may interfere with the signaling required for kidney formation. Two alternatively spliced transcript variants that encode different proteins have been found for this gene.

Entrez Gene ID

9653

UniProt ID

Q7LGA3

Alternative Names

Heparan Sulfate 2-O-Sulfotransferase 1; 2-O-Sulfotransferase; 2OST; EC 2.8.2.-; DJ604K5.2; KIAA0448; EC 2.8.2; HS2ST

Function

Catalyzes the transfer of sulfate to the C2-position of selected hexuronic acid residues within the maturing heparan sulfate (HS). 2-O-sulfation within HS, particularly of iduronate residues, is essential for HS to participate in a variety of high-affinity ligand-binding interactions and signaling processes. Mediates 2-O-sulfation of both L-iduronyl and D-glucuronyl residues (By similarity).

Biological Process

Glycosaminoglycan biosynthetic process Source: Reactome
Heparan sulfate proteoglycan biosynthetic process, enzymatic modification Source: GO_Central
Heparan sulfate proteoglycan biosynthetic process, polysaccharide chain biosynthetic process Source: GO_Central

Cellular Location

Golgi apparatus membrane

Involvement in disease

Neurofacioskeletal syndrome with or without renal agenesis (NFSRA):
An autosomal recessive syndrome characterized by developmental delay and/or intellectual disability, corpus callosum agenesis or hypoplasia, flexion contractures, brachydactyly of hands and feet with broad fingertips and toes, and dysmorphic features such as coarse face, upslanted palpebral fissures, broad nasal tip and wide mouth. Some patients manifest unilateral or bilateral renal agenesis.

Topology

Cytoplasmic: 1-11
Helical: 12-28
Lumenal: 29-356

PTM

N-glycosylated.

Yang, G., Jiang, J., Yin, R., Li, Z., Li, L., Gao, F., ... & Zhan, X. (2022). Two novel predictive biomarkers for osteosarcoma and glycolysis pathways: A profiling study on HS2ST1 and SDC3. Medicine, 101(36), e30192.

Berillo, O., Ouerd, S., Idris-Khodja, N., Rehman, A., Richer, C., Sinnett, D., ... & Schiffrin, E. L. (2021). Chromosome 2 Fragment substitutions in dahl salt-sensitive rats and RNA sequencing identified Enpep and Hs2st1 as vascular inflammatory modulators. Hypertension, 77(1), 178-189.

Ahronian, L. G., Fenoglio, S., Das, N., Aird, D., Guerin, D., Whittington, D., ... & Sjin, R. T. T. (2021). Abstract P146: Loss of HS2ST1 cooperates with MAPK inhibition to impair growth of mesenchymal KRAS mutant NSCLC. Molecular Cancer Therapeutics, 20(12_Supplement), P146-P146.

Teixeira, F. C., Vijaya Kumar, A., Kumar Katakam, S., Cocola, C., Pelucchi, P., Graf, M., ... & Götte, M. (2020). The heparan sulfate sulfotransferases HS2ST1 and HS3ST2 are novel regulators of breast cancer stem-cell properties. Frontiers in Cell and Developmental Biology, 8, 559554.

Vijaya Kumar, A., Brézillon, S., Untereiner, V., Sockalingum, G. D., Kumar Katakam, S., Mohamed, H. T., ... & Götte, M. (2020). HS2ST1‐dependent signaling pathways determine breast cancer cell viability, matrix interactions, and invasive behavior. Cancer Science, 111(8), 2907-2922.

Schneeberger, P. E., von Elsner, L., Barker, E. L., Meinecke, P., Marquardt, I., Alawi, M., ... & Kutsche, K. (2020). Bi-allelic pathogenic variants in HS2ST1 cause a syndrome characterized by developmental delay and corpus callosum, skeletal, and renal abnormalities. The American Journal of Human Genetics, 107(6), 1044-1061.

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme-Linked Immunospot (ELISpot)
Proteogenomics
Other Protocols

Isotype control

For research use only. Not intended for any clinical use.

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