Search :
Sign in or Register  
Welcome Sign in or Don't have an account?Register

Mouse Anti-HSD3B2 (AA 33-122) Recombinant Antibody (CBFYH-2024) (CBMAB-H3038-FY)


See all HSD3B2 antibodies
Online Inquiry

Summary

Host Animal
Mouse
Specificity
Human
Clone
CBFYH-2024
Antibody Isotype
IgG2a, κ
Application
ELISA, WB

Basic Information

Immunogen
Recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Immunogen sequence: ALDKAFRPEL REEFSKLQNR TKLTVLEGDI LDEPFLKRAC QDVSVVIHTA CIIDVFGVTH RESIMNVNVK GTQLLLEACV QASVPVFIYT
Specificity
Human
Antibody Isotype
IgG2a, κ
Clonality
Monoclonal
Application Notes
The COA includes recommended starting dilutions, optimal dilutions should be determined by the end user.

Formulations & Storage [For reference only, actual COA shall prevail!]

Format
Liquid
Storage
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Epitope
AA 33-122

Target

Full Name
hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 2
Introduction
The protein encoded by this gene is a bifunctional enzyme that catalyzes the oxidative conversion of delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. It plays a crucial role in the biosynthesis of all classes of hormonal steroids. This gene is predominantly expressed in the adrenals and the gonads. Mutations in this gene are associated with 3-beta-hydroxysteroid dehydrogenase, type II, deficiency. Alternatively spliced transcript variants have been found for this gene.
Entrez Gene ID
3284
UniProt ID
P26439
Alternative Names
Hydroxy-Delta-5-Steroid Dehydrogenase, 3 Beta- And Steroid Delta-Isomerase 2; 3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type II; Short Chain Dehydrogenase/Reductase Family 11E, Member 2; 3-Beta-HSD Adrenal And Gonadal Type; 3-Beta-HSD II; 3 Beta-Hydroxysteroid Dehydrogenase Type II, Delta 5-Delta 4-Isomerase Type II, 3 Beta-HSD Type II; 3 Beta-Hydroxysteroid Dehydrogenase/Delta 5-->4-Isomerase Type 2; 3-Beta-Hydroxy-Delta(5)-Steroid Dehydrogenase; 3-Beta-Hydroxy-5-Ene Steroid Dehydrogenase
Function
3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta5-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids.
Biological Process
Androgen biosynthetic process Source: UniProtKB
C21-steroid hormone metabolic process Source: GO_Central
Hippocampus development Source: GO_Central
Response to corticosterone Source: GO_Central
Steroid biosynthetic process Source: UniProtKB
Cellular Location
Mitochondrion membrane; Endoplasmic reticulum membrane
Involvement in disease
Adrenal hyperplasia 2 (AH2):
A form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: 'salt wasting' (SW, the most severe type), 'simple virilizing' (SV, less severely affected patients), with normal aldosterone biosynthesis, 'non-classic form' or late-onset (NC or LOAH) and 'cryptic' (asymptomatic). In AH2, virilization is much less marked or does not occur. AH2 is frequently lethal in early life. Mild HSD3B2 deficiency in hyperandrogenic females is associated with characteristic traits of polycystic ovary syndrome, such as insulin resistance and luteinizing hormone hypersecretion.
Topology
Helical: 287-307
More Infomation

Almaramhy, H. H., Abdul Samad, F., Al-Harbi, G., Zaytuni, D., Imam, S. N., Masoodi, T., & Shamsi, M. B. (2023). Identification of a novel candidate HSD3B2 gene variant for familial hypospadias by whole-exome sequencing. Frontiers in Genetics, 14, 1106933.

Makretskaya, N., Kalinchenko, N., Tebieva, I., Ionova, S., Zinchenko, R., Marakhonov, A., & Tiulpakov, A. (2023). High carrier frequency of a nonsense p. Trp230* variant in HSD3B2 gene in Ossetians. Frontiers in Endocrinology, 14, 1146768.

Mellone, S., Bertelli, E., Roviglione, B., Vurchio, D., Ronzani, S., Secco, A., ... & Giordano, M. (2022). Co-occurrence of a pathogenic HSD3B2 variant and a duplication on 10q22. 3-q23. 2 detected in newborn twins with salt-wasting congenital adrenal hyperplasia. Genes, 13(12), 2190.

Fanis, P., Neocleous, V., Kosta, K., Karipiadou, A., Hartmann, M. F., Wudy, S. A., ... & Papagianni, M. (2021). Late diagnosis of 3β-Hydroxysteroid dehydrogenase deficiency: the pivotal role of gas chromatography-mass spectrometry urinary steroid metabolome analysis and a novel homozygous nonsense mutation in the HSD3B2 gene. Journal of Pediatric Endocrinology and Metabolism, 34(1), 131-136.

Giri, D., Bockenhauer, D., Deshpande, C., Achermann, J. C., Taylor, N. F., Rumsby, G., ... & Ajzensztejn, M. (2020). Co-Existence of Congenital Adrenal Hyperplasia and Bartter Syndrome due to Maternal Uniparental Isodisomy of HSD3B2 and CLCNKB Mutations. Hormone Research in Paediatrics, 93(2), 137-142.

Yu, J., Liu, Y., Zhang, D., Zhai, D., Song, L., Cai, Z., & Yu, C. (2019). Baicalin inhibits recruitment of GATA1 to the HSD3B2 promoter and reverses hyperandrogenism of PCOS. Journal of Endocrinology, 240(3), 497-507.

Baquedano, M. S., Guercio, G., Costanzo, M., Marino, R., Rivarola, M. A., & Belgorosky, A. (2018). Mutation of HSD3B2 gene and fate of dehydroepiandrosterone. Vitamins and Hormones, 108, 75-123.

Osiński, M., Wirstlein, P., Wender-Ożegowska, E., Mikołajczyk, M., Jagodziński, P. P., & Szczepańska, M. (2018). HSD3B2, HSD17B1, HSD17B2, ESR1, ESR2 and AR expression in infertile women with endometriosis. Ginekologia polska, 89(3), 125-134.

Neubauer, E., Latif, M., Krause, J., Heumann, A., Armbrust, M., Luehr, C., ... & Büscheck, F. (2018). Up regulation of the steroid hormone synthesis regulator HSD3B2 is linked to early PSA recurrence in prostate cancer. Experimental and molecular pathology, 105(1), 50-56.

Zhang, C., Wang, M., He, N., Ahmed, M. F., Wang, Y., Zhao, R., ... & Li, B. (2018). Hsd3b2 associated in modulating steroid hormone synthesis pathway regulates the differentiation of chicken embryonic stem cells into spermatogonial stem cells. Journal of Cellular Biochemistry, 119(1), 1111-1121.

Ask a question We look forward to hearing from you.
0 reviews or Q&As

Purchasing FAQs
Technical FAQs

Loading...
Have you used Mouse Anti-HSD3B2 (AA 33-122) Recombinant Antibody (CBFYH-2024)?
Submit a review and get a Coupon or an Amazon gift card. 20% off Coupon $30 eGift Card
Submit a review
Loading...
For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

Learn more

Documents

Request bulk or custom quote Online Inquiry
Second antibody and isotype control

Contact us

  • Tel: (USA)
  • (UK)
  • Fax:
  • Email:

Submit A Review

Online Inquiry