Rabbit Anti-HSP90AA1 Recombinant Antibody (
4B5) (V2LY-0725-LY786)

Name: Rabbit Anti-HSP90AA1 Recombinant Antibody (4B5)
SKU: V2LY-0725-LY786
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-HSP90AA1 Recombinant Antibody (4B5) (V2LY-0725-LY786) in WB

Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate, Rat brain tissue
All lanes: Hsp90 alpha antibody at 0.8μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 85, 99 KDa
Observed band size: 85 KDa

Rabbit Anti-HSP90AA1 Recombinant Antibody (4B5) (V2LY-0725-LY786) in IHC

IHC image of V2LY-0725-LY786 diluted at 1:80 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Rabbit Anti-HSP90AA1 Recombinant Antibody (4B5) (V2LY-0725-LY786) in IHC

IHC image of V2LY-0725-LY786 diluted at 1:80 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Rabbit Anti-HSP90AA1 Recombinant Antibody (4B5) (V2LY-0725-LY786) in IF

Immunofluorescence staining of NIH/3T3 cells with V2LY-0725-LY786 at 1:26, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Rabbit Anti-HSP90AA1 Recombinant Antibody (4B5) (V2LY-0725-LY786) in IP

Immunoprecipitating Hsp90 in Hela whole cell lysate
Lane 1: Rabbit control IgG instead of V2LY-0725-LY786 in Hela whole cell lysate.
For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: V2LY-0725-LY786 (3μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

4B5

Application

ELISA, WB, IHC, IF, IP

Immunogen

A synthesized peptide derived from human HSP90AA1.

Host Species

Rabbit

Specificity

Human, Rat

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
WB	1:500-1:5,000
IHC	1:50-1:200
IF	1:20-1:200
IP	1:200-1:1,000

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Heat Shock Protein 90 Alpha Family Class A Member 1

Entrez Gene ID

3320

UniProt ID

P07900

Alternative Names

Heat Shock Protein 90 Alpha Family Class A Member 1; Heat Shock Protein 90kDa Alpha (Cytosolic), Class A Member 1; Lipopolysaccharide-Associated Protein 2; Heat Shock 90kDa Protein 1, Alpha; Renal Carcinoma Antigen NY-REN-38; Heat Shock 90kD Protein 1, Alpha; LPS-Associated Protein 2; Heat Shock 86 KDa; HSP 86; HSP90A; HSPC1; HSPCA; HSP86; LAP-2; Heat Shock Protein 90kDa Alpha Family Class A Member 1; Epididymis Secretory Sperm Binding Protein Li 65p;

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

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We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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