Rabbit Anti-HSP90AA1 Recombinant Antibody (
4F8) (V2LY-0725-LY787)

Name: Rabbit Anti-HSP90AA1 Recombinant Antibody (4F8)
SKU: V2LY-0725-LY787
Rating: 5 (1 reviews)

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Tested Data

Rabbit Anti-HSP90AA1 Recombinant Antibody (4F8) (V2LY-0725-LY787) in IHC

IHC image of V2LY-0725-LY787 diluted at 1:87.5 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Rabbit Anti-HSP90AA1 Recombinant Antibody (4F8) (V2LY-0725-LY787) in IHC

IHC image of V2LY-0725-LY787 diluted at 1:87.5 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Rabbit Anti-HSP90AA1 Recombinant Antibody (4F8) (V2LY-0725-LY787) in IF

Immunofluorescence staining of Hela cells with V2LY-0725-LY787 at 1:29, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Datasheet

SDS

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Basic Information

Host Animal

Rabbit

Clone

4F8

Application

ELISA, IHC, IF

Immunogen

A synthesized peptide derived from human HSP90AA1.

Host Species

Rabbit

Specificity

Human

Antibody Isotype

IgG

Clonality

Monoclonal Antibody

Application Notes

Application	Note
IHC	1:50-1:200
IF	1:20-1:200

Formulations & Storage [For reference only, actual COA shall prevail!]

Format

Liquid

Buffer

PBS, glycerol

Preservative

Sodium azide

Concentration

Batch dependent

Purity

> 95% Purity determined by SDS-PAGE.

Storage

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freezethaw cycles.

More Infomation

Target

Full Name

Heat Shock Protein 90 Alpha Family Class A Member 1

Entrez Gene ID

3320

UniProt ID

P07900

Alternative Names

Heat Shock Protein 90 Alpha Family Class A Member 1; Heat Shock Protein 90kDa Alpha (Cytosolic), Class A Member 1; Lipopolysaccharide-Associated Protein 2; Heat Shock 90kDa Protein 1, Alpha; Renal Carcinoma Antigen NY-REN-38; Heat Shock 90kD Protein 1, Alpha; LPS-Associated Protein 2; Heat Shock 86 KDa; HSP 86; HSP90A; HSPC1; HSPCA; HSP86; LAP-2; Heat Shock Protein 90kDa Alpha Family Class A Member 1; Epididymis Secretory Sperm Binding Protein Li 65p;

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Please try the standard protocols which include: protocols, troubleshooting and guide.

Enzyme-linked Immunosorbent Assay (ELISA)
Flow Cytometry
Immunofluorescence (IF)
Immunohistochemistry (IHC)
Immunoprecipitation (IP)
Western Blot (WB)
Enzyme Linked Immunospot (ELISpot)
Proteogenomic
Other Protocols

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Isotype control

For research use only. Not intended for any clinical use.

Custom Antibody Labeling

We also offer labeled antibodies developed using our catalog antibody products and nonfluorescent conjugates (HRP, AP, Biotin, etc.) or fluorescent conjugates (Alexa Fluor, FITC, TRITC, Rhodamine, Texas Red, R-PE, APC, Qdot Probes, Pacific Dyes, etc.).

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